Cargando…
Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer
BACKGROUND: Frozen-thawed embryo transfer (FET) is increasingly available for the improvement of the success rate of assisted reproductive technologies other than fresh embryo transfer (ET). There have been numerous findings that FET provides better obstetric and perinatal outcomes. However, the bir...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543431/ https://www.ncbi.nlm.nih.gov/pubmed/28785370 http://dx.doi.org/10.1186/s13148-017-0379-6 |
_version_ | 1783255147591237632 |
---|---|
author | Hiura, Hitoshi Hattori, Hiromitsu Kobayashi, Norio Okae, Hiroaki Chiba, Hatsune Miyauchi, Naoko Kitamura, Akane Kikuchi, Hiroyuki Yoshida, Hiroaki Arima, Takahiro |
author_facet | Hiura, Hitoshi Hattori, Hiromitsu Kobayashi, Norio Okae, Hiroaki Chiba, Hatsune Miyauchi, Naoko Kitamura, Akane Kikuchi, Hiroyuki Yoshida, Hiroaki Arima, Takahiro |
author_sort | Hiura, Hitoshi |
collection | PubMed |
description | BACKGROUND: Frozen-thawed embryo transfer (FET) is increasingly available for the improvement of the success rate of assisted reproductive technologies other than fresh embryo transfer (ET). There have been numerous findings that FET provides better obstetric and perinatal outcomes. However, the birth weight of infants conceived using FET is heavier than that of those conceived via ET. In addition, some reports have suggested that FET is associated with perinatal diseases such as placenta accreta and pregnancy-induced hypertension (PIH). RESULTS: In this study, we compared the microRNA (miRNA) expression profiles in term placentae derived from FET, ET, and spontaneous pregnancy (SP). We identified four miRNAs, miR-130a-3p, miR-149-5p, miR-423-5p, and miR-487b-3p, that were significantly downregulated in FET placentae compared with those from SP and ET. We found that DNA methylation of MEG3-DMR, not but IG-DMR, was associated with miRNA expression of the DLK1-DIO3 imprinted domain in the human placenta. In functional analyses, GO terms and signaling pathways related to positive regulation of gene expression, growth, development, cell migration, and type II diabetes mellitus (T2DM) were enriched. CONCLUSIONS: This study supports the hypothesis that the process of FET may increase exposure of epigenome to external influences. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13148-017-0379-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5543431 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55434312017-08-07 Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer Hiura, Hitoshi Hattori, Hiromitsu Kobayashi, Norio Okae, Hiroaki Chiba, Hatsune Miyauchi, Naoko Kitamura, Akane Kikuchi, Hiroyuki Yoshida, Hiroaki Arima, Takahiro Clin Epigenetics Research BACKGROUND: Frozen-thawed embryo transfer (FET) is increasingly available for the improvement of the success rate of assisted reproductive technologies other than fresh embryo transfer (ET). There have been numerous findings that FET provides better obstetric and perinatal outcomes. However, the birth weight of infants conceived using FET is heavier than that of those conceived via ET. In addition, some reports have suggested that FET is associated with perinatal diseases such as placenta accreta and pregnancy-induced hypertension (PIH). RESULTS: In this study, we compared the microRNA (miRNA) expression profiles in term placentae derived from FET, ET, and spontaneous pregnancy (SP). We identified four miRNAs, miR-130a-3p, miR-149-5p, miR-423-5p, and miR-487b-3p, that were significantly downregulated in FET placentae compared with those from SP and ET. We found that DNA methylation of MEG3-DMR, not but IG-DMR, was associated with miRNA expression of the DLK1-DIO3 imprinted domain in the human placenta. In functional analyses, GO terms and signaling pathways related to positive regulation of gene expression, growth, development, cell migration, and type II diabetes mellitus (T2DM) were enriched. CONCLUSIONS: This study supports the hypothesis that the process of FET may increase exposure of epigenome to external influences. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13148-017-0379-6) contains supplementary material, which is available to authorized users. BioMed Central 2017-08-03 /pmc/articles/PMC5543431/ /pubmed/28785370 http://dx.doi.org/10.1186/s13148-017-0379-6 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Hiura, Hitoshi Hattori, Hiromitsu Kobayashi, Norio Okae, Hiroaki Chiba, Hatsune Miyauchi, Naoko Kitamura, Akane Kikuchi, Hiroyuki Yoshida, Hiroaki Arima, Takahiro Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer |
title | Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer |
title_full | Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer |
title_fullStr | Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer |
title_full_unstemmed | Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer |
title_short | Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer |
title_sort | genome-wide microrna expression profiling in placentae from frozen-thawed blastocyst transfer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543431/ https://www.ncbi.nlm.nih.gov/pubmed/28785370 http://dx.doi.org/10.1186/s13148-017-0379-6 |
work_keys_str_mv | AT hiurahitoshi genomewidemicrornaexpressionprofilinginplacentaefromfrozenthawedblastocysttransfer AT hattorihiromitsu genomewidemicrornaexpressionprofilinginplacentaefromfrozenthawedblastocysttransfer AT kobayashinorio genomewidemicrornaexpressionprofilinginplacentaefromfrozenthawedblastocysttransfer AT okaehiroaki genomewidemicrornaexpressionprofilinginplacentaefromfrozenthawedblastocysttransfer AT chibahatsune genomewidemicrornaexpressionprofilinginplacentaefromfrozenthawedblastocysttransfer AT miyauchinaoko genomewidemicrornaexpressionprofilinginplacentaefromfrozenthawedblastocysttransfer AT kitamuraakane genomewidemicrornaexpressionprofilinginplacentaefromfrozenthawedblastocysttransfer AT kikuchihiroyuki genomewidemicrornaexpressionprofilinginplacentaefromfrozenthawedblastocysttransfer AT yoshidahiroaki genomewidemicrornaexpressionprofilinginplacentaefromfrozenthawedblastocysttransfer AT arimatakahiro genomewidemicrornaexpressionprofilinginplacentaefromfrozenthawedblastocysttransfer |