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Germline factor DDX4 functions in blood‐derived cancer cell phenotypes

DDX4 (the human ortholog of Drosophila Vasa) is an RNA helicase and is present in the germ lines of all metazoans tested. It was historically thought to be expressed specifically in germline, but with additional organisms studied, it is now clear that in some animals DDX4/Vasa functions outside of t...

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Autores principales: Schudrowitz, Natalie, Takagi, Satoshi, Wessel, Gary M., Yajima, Mamiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543511/
https://www.ncbi.nlm.nih.gov/pubmed/28612512
http://dx.doi.org/10.1111/cas.13299
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author Schudrowitz, Natalie
Takagi, Satoshi
Wessel, Gary M.
Yajima, Mamiko
author_facet Schudrowitz, Natalie
Takagi, Satoshi
Wessel, Gary M.
Yajima, Mamiko
author_sort Schudrowitz, Natalie
collection PubMed
description DDX4 (the human ortholog of Drosophila Vasa) is an RNA helicase and is present in the germ lines of all metazoans tested. It was historically thought to be expressed specifically in germline, but with additional organisms studied, it is now clear that in some animals DDX4/Vasa functions outside of the germline, in a variety of somatic cells in the embryo and in the adult. In this report, we document that DDX4 is widely expressed in soma‐derived cancer cell lines, including myeloma (IM‐9) and leukemia (THP‐1) cells. In these cells, the DDX4 protein localized to the mitotic spindle, consistent with findings in other somatic cell functions, and its knockout in IM‐9 cells compromised cell proliferation and migration activities, and downregulated several cell cycle/oncogene factors such as CyclinB and the transcription factor E2F1. These results suggest that DDX4 positively regulates cell cycle progression of diverse somatic‐derived blood cancer cells, implying its broad contributions to the cancer cell phenotype and serves as a potential new target for chemotherapy.
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spelling pubmed-55435112017-08-09 Germline factor DDX4 functions in blood‐derived cancer cell phenotypes Schudrowitz, Natalie Takagi, Satoshi Wessel, Gary M. Yajima, Mamiko Cancer Sci Original Articles DDX4 (the human ortholog of Drosophila Vasa) is an RNA helicase and is present in the germ lines of all metazoans tested. It was historically thought to be expressed specifically in germline, but with additional organisms studied, it is now clear that in some animals DDX4/Vasa functions outside of the germline, in a variety of somatic cells in the embryo and in the adult. In this report, we document that DDX4 is widely expressed in soma‐derived cancer cell lines, including myeloma (IM‐9) and leukemia (THP‐1) cells. In these cells, the DDX4 protein localized to the mitotic spindle, consistent with findings in other somatic cell functions, and its knockout in IM‐9 cells compromised cell proliferation and migration activities, and downregulated several cell cycle/oncogene factors such as CyclinB and the transcription factor E2F1. These results suggest that DDX4 positively regulates cell cycle progression of diverse somatic‐derived blood cancer cells, implying its broad contributions to the cancer cell phenotype and serves as a potential new target for chemotherapy. John Wiley and Sons Inc. 2017-07-11 2017-08 /pmc/articles/PMC5543511/ /pubmed/28612512 http://dx.doi.org/10.1111/cas.13299 Text en © 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Schudrowitz, Natalie
Takagi, Satoshi
Wessel, Gary M.
Yajima, Mamiko
Germline factor DDX4 functions in blood‐derived cancer cell phenotypes
title Germline factor DDX4 functions in blood‐derived cancer cell phenotypes
title_full Germline factor DDX4 functions in blood‐derived cancer cell phenotypes
title_fullStr Germline factor DDX4 functions in blood‐derived cancer cell phenotypes
title_full_unstemmed Germline factor DDX4 functions in blood‐derived cancer cell phenotypes
title_short Germline factor DDX4 functions in blood‐derived cancer cell phenotypes
title_sort germline factor ddx4 functions in blood‐derived cancer cell phenotypes
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543511/
https://www.ncbi.nlm.nih.gov/pubmed/28612512
http://dx.doi.org/10.1111/cas.13299
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