Analysis of Genetic Variation of Two NADC30-like Strains of Porcine Reproductive and Respiratory Syndrome Virus in China

BACKGROUND: NADC30-like strains of porcine reproductive and respiratory syndrome virus first appeared in Chinese swine herds in 2012. OBJECTIVE AND METHOD: To explore the possible genetic diversity of these strains, we sequenced and analyzed the complete genomes of two NADC30-like strains. These iso...

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Autores principales: Zhou, Zhi, Wu, Jiajun, Zhang, Shuo, Hou, Bo, Han, Tao, Wang, Jing, Xu, Qi, Wang, Dongyue, Liu, Yinyi, Xin, Shengpeng, Zhai, Xinyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543627/
https://www.ncbi.nlm.nih.gov/pubmed/28839509
http://dx.doi.org/10.2174/1874357901711010090
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author Zhou, Zhi
Wu, Jiajun
Zhang, Shuo
Hou, Bo
Han, Tao
Wang, Jing
Xu, Qi
Wang, Dongyue
Liu, Yinyi
Xin, Shengpeng
Zhai, Xinyan
author_facet Zhou, Zhi
Wu, Jiajun
Zhang, Shuo
Hou, Bo
Han, Tao
Wang, Jing
Xu, Qi
Wang, Dongyue
Liu, Yinyi
Xin, Shengpeng
Zhai, Xinyan
author_sort Zhou, Zhi
collection PubMed
description BACKGROUND: NADC30-like strains of porcine reproductive and respiratory syndrome virus first appeared in Chinese swine herds in 2012. OBJECTIVE AND METHOD: To explore the possible genetic diversity of these strains, we sequenced and analyzed the complete genomes of two NADC30-like strains. These isolates shared 95.4% homology with NADC30. RESULT: The two strains displayed a discontinuous deletion of 131 amino acids in NSP2, mutations of amino acids in GP3 and GP5, and a 3-nucleotide deletion in the 3′ untranslated region. Phylogenetic analysis showed that the two isolates formed a new branch and clustered in a subgroup with NADC30 isolates from North America. CONCLUSION: We conclude that the above two NADC30-like strains may have been introduced from North America to China, where they acquired new genetic diversity.
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spelling pubmed-55436272017-08-24 Analysis of Genetic Variation of Two NADC30-like Strains of Porcine Reproductive and Respiratory Syndrome Virus in China Zhou, Zhi Wu, Jiajun Zhang, Shuo Hou, Bo Han, Tao Wang, Jing Xu, Qi Wang, Dongyue Liu, Yinyi Xin, Shengpeng Zhai, Xinyan Open Virol J Article BACKGROUND: NADC30-like strains of porcine reproductive and respiratory syndrome virus first appeared in Chinese swine herds in 2012. OBJECTIVE AND METHOD: To explore the possible genetic diversity of these strains, we sequenced and analyzed the complete genomes of two NADC30-like strains. These isolates shared 95.4% homology with NADC30. RESULT: The two strains displayed a discontinuous deletion of 131 amino acids in NSP2, mutations of amino acids in GP3 and GP5, and a 3-nucleotide deletion in the 3′ untranslated region. Phylogenetic analysis showed that the two isolates formed a new branch and clustered in a subgroup with NADC30 isolates from North America. CONCLUSION: We conclude that the above two NADC30-like strains may have been introduced from North America to China, where they acquired new genetic diversity. Bentham Open 2017-06-30 /pmc/articles/PMC5543627/ /pubmed/28839509 http://dx.doi.org/10.2174/1874357901711010090 Text en © 2017 zhou et al. https://creativecommons.org/licenses/by/4.0/legalcode This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Zhou, Zhi
Wu, Jiajun
Zhang, Shuo
Hou, Bo
Han, Tao
Wang, Jing
Xu, Qi
Wang, Dongyue
Liu, Yinyi
Xin, Shengpeng
Zhai, Xinyan
Analysis of Genetic Variation of Two NADC30-like Strains of Porcine Reproductive and Respiratory Syndrome Virus in China
title Analysis of Genetic Variation of Two NADC30-like Strains of Porcine Reproductive and Respiratory Syndrome Virus in China
title_full Analysis of Genetic Variation of Two NADC30-like Strains of Porcine Reproductive and Respiratory Syndrome Virus in China
title_fullStr Analysis of Genetic Variation of Two NADC30-like Strains of Porcine Reproductive and Respiratory Syndrome Virus in China
title_full_unstemmed Analysis of Genetic Variation of Two NADC30-like Strains of Porcine Reproductive and Respiratory Syndrome Virus in China
title_short Analysis of Genetic Variation of Two NADC30-like Strains of Porcine Reproductive and Respiratory Syndrome Virus in China
title_sort analysis of genetic variation of two nadc30-like strains of porcine reproductive and respiratory syndrome virus in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543627/
https://www.ncbi.nlm.nih.gov/pubmed/28839509
http://dx.doi.org/10.2174/1874357901711010090
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