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Non-Coding RNAs are Differentially Expressed by Nocardia brasiliensis in Vitro and in Experimental Actinomycetoma

INTRODUCTION: Nocardia spp. are common soil-inhabiting bacteria that frequently infect humans through traumatic injuries or inhalation routes and cause infections, such as actinomycetoma and nocardiosis, respectively. Nocardia brasiliensis is the main aetiological agent of actinomycetoma in various...

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Autores principales: Cruz-Rabadán, Josué S., Miranda-Ríos, Juan, Espín-Ocampo, Guadalupe, Méndez-Tovar, Luis J., Maya-Pineda, Héctor Rubén, Hernández-Hernández, Francisca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543724/
https://www.ncbi.nlm.nih.gov/pubmed/28839491
http://dx.doi.org/10.2174/1874285801711010112
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author Cruz-Rabadán, Josué S.
Miranda-Ríos, Juan
Espín-Ocampo, Guadalupe
Méndez-Tovar, Luis J.
Maya-Pineda, Héctor Rubén
Hernández-Hernández, Francisca
author_facet Cruz-Rabadán, Josué S.
Miranda-Ríos, Juan
Espín-Ocampo, Guadalupe
Méndez-Tovar, Luis J.
Maya-Pineda, Héctor Rubén
Hernández-Hernández, Francisca
author_sort Cruz-Rabadán, Josué S.
collection PubMed
description INTRODUCTION: Nocardia spp. are common soil-inhabiting bacteria that frequently infect humans through traumatic injuries or inhalation routes and cause infections, such as actinomycetoma and nocardiosis, respectively. Nocardia brasiliensis is the main aetiological agent of actinomycetoma in various countries. Many bacterial non-coding RNAs are regulators of genes associated with virulence factors. OBJECTIVE: The aim of this work was to identify non-coding RNAs (ncRNAs) expressed during infection conditions and in free-living form (in vitro) in Nocardia brasiliensis. METHODS AND RESULT: The N. brasiliensis transcriptome (predominately < 200 nucleotides) was determined by RNA next-generation sequencing in both conditions. A total of seventy ncRNAs were identified in both conditions. Among these ncRNAs, 18 were differentially expressed, 12 were located within intergenic regions, and 2 were encoded as antisense of 2 different genes. Finally, 10 of these ncRNAs were studied by rapid amplification of cDNA ends and/or quantitative reverse transcription polymerase chain reaction. Interestingly, 3 transcripts corresponded to tRNA-derived fragments (tRNAs(Cys, Met, Thr)), and one transcript was overlapped between an intergenic region and the 5´end of the 23S rRNA. Expression of these last four transcripts was increased during N. brasiliensis infection compared with the in vitro conditions. CONCLUSION: The results of this work suggest a possible role for these transcripts in the regulation of virulence genes in actinomycetoma pathogenesis.
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spelling pubmed-55437242017-08-24 Non-Coding RNAs are Differentially Expressed by Nocardia brasiliensis in Vitro and in Experimental Actinomycetoma Cruz-Rabadán, Josué S. Miranda-Ríos, Juan Espín-Ocampo, Guadalupe Méndez-Tovar, Luis J. Maya-Pineda, Héctor Rubén Hernández-Hernández, Francisca Open Microbiol J Article INTRODUCTION: Nocardia spp. are common soil-inhabiting bacteria that frequently infect humans through traumatic injuries or inhalation routes and cause infections, such as actinomycetoma and nocardiosis, respectively. Nocardia brasiliensis is the main aetiological agent of actinomycetoma in various countries. Many bacterial non-coding RNAs are regulators of genes associated with virulence factors. OBJECTIVE: The aim of this work was to identify non-coding RNAs (ncRNAs) expressed during infection conditions and in free-living form (in vitro) in Nocardia brasiliensis. METHODS AND RESULT: The N. brasiliensis transcriptome (predominately < 200 nucleotides) was determined by RNA next-generation sequencing in both conditions. A total of seventy ncRNAs were identified in both conditions. Among these ncRNAs, 18 were differentially expressed, 12 were located within intergenic regions, and 2 were encoded as antisense of 2 different genes. Finally, 10 of these ncRNAs were studied by rapid amplification of cDNA ends and/or quantitative reverse transcription polymerase chain reaction. Interestingly, 3 transcripts corresponded to tRNA-derived fragments (tRNAs(Cys, Met, Thr)), and one transcript was overlapped between an intergenic region and the 5´end of the 23S rRNA. Expression of these last four transcripts was increased during N. brasiliensis infection compared with the in vitro conditions. CONCLUSION: The results of this work suggest a possible role for these transcripts in the regulation of virulence genes in actinomycetoma pathogenesis. Bentham Open 2017-06-30 /pmc/articles/PMC5543724/ /pubmed/28839491 http://dx.doi.org/10.2174/1874285801711010112 Text en © 2017 Cruz-Rabadán et al. https://creativecommons.org/licenses/by/4.0/legalcode This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Cruz-Rabadán, Josué S.
Miranda-Ríos, Juan
Espín-Ocampo, Guadalupe
Méndez-Tovar, Luis J.
Maya-Pineda, Héctor Rubén
Hernández-Hernández, Francisca
Non-Coding RNAs are Differentially Expressed by Nocardia brasiliensis in Vitro and in Experimental Actinomycetoma
title Non-Coding RNAs are Differentially Expressed by Nocardia brasiliensis in Vitro and in Experimental Actinomycetoma
title_full Non-Coding RNAs are Differentially Expressed by Nocardia brasiliensis in Vitro and in Experimental Actinomycetoma
title_fullStr Non-Coding RNAs are Differentially Expressed by Nocardia brasiliensis in Vitro and in Experimental Actinomycetoma
title_full_unstemmed Non-Coding RNAs are Differentially Expressed by Nocardia brasiliensis in Vitro and in Experimental Actinomycetoma
title_short Non-Coding RNAs are Differentially Expressed by Nocardia brasiliensis in Vitro and in Experimental Actinomycetoma
title_sort non-coding rnas are differentially expressed by nocardia brasiliensis in vitro and in experimental actinomycetoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543724/
https://www.ncbi.nlm.nih.gov/pubmed/28839491
http://dx.doi.org/10.2174/1874285801711010112
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