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Comparison of Commercially Available Blood Collection Tubes Containing Sodium Citrate and Hirudin in Platelet Aggregation Testing

BACKGROUND: Platelet reactivity assessment is an important tool in both the causal determination of bleeding diathesis as well as in the evaluation of the efficacy of anti-platelet therapy in patients at risk of thrombosis. Sodium citrate is the most widely used anticoagulant for hemostasis investig...

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Autores principales: Janse Van Rensburg, Walter J., van der Merwe, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543979/
https://www.ncbi.nlm.nih.gov/pubmed/28747619
http://dx.doi.org/10.12659/MSMBR.905246
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author Janse Van Rensburg, Walter J.
van der Merwe, Patricia
author_facet Janse Van Rensburg, Walter J.
van der Merwe, Patricia
author_sort Janse Van Rensburg, Walter J.
collection PubMed
description BACKGROUND: Platelet reactivity assessment is an important tool in both the causal determination of bleeding diathesis as well as in the evaluation of the efficacy of anti-platelet therapy in patients at risk of thrombosis. Sodium citrate is the most widely used anticoagulant for hemostasis investigations. However, some doubt exists over the suitability of sodium citrate in platelet function testing. Hirudin has been suggested as a superior replacement. Nevertheless, only 1 study compared citrated and hirudin treated samples with light transmission aggregometry. Therefore, limited evidence exists to conclusively prove the supremacy of hirudin over sodium citrate in light transmission aggregometry. The aim of our study was to compare citrated and hirudin treated samples, collected in commercially available blood collection tubes, using the 5 most common agonists, with light transmission aggregometry. MATERIAL/METHODS: Blood was obtained from 20 healthy volunteers. Platelet counts were performed on platelet-rich plasma. Light transmission aggregometry was performed within 4 h of sample collection using ADP, collagen, arachidonic acid, epinephrine, and ristocetin as agonists. RESULTS: Platelet counts for the respective anticoagulants did not differ significantly. ADP-induced aggregation was comparable between the samples. However, among all the agonists, hirudin-treated platelets had significantly weaker aggregatory responses. CONCLUSIONS: Commercially available sodium citrate should remain the anticoagulant of choice for routine platelet function testing in our setting. However, the time limitation associated with the use of sodium citrate in platelet function testing remains a concern. Thus, alternative anticoagulants should still be explored.
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spelling pubmed-55439792017-08-15 Comparison of Commercially Available Blood Collection Tubes Containing Sodium Citrate and Hirudin in Platelet Aggregation Testing Janse Van Rensburg, Walter J. van der Merwe, Patricia Med Sci Monit Basic Res Human Study BACKGROUND: Platelet reactivity assessment is an important tool in both the causal determination of bleeding diathesis as well as in the evaluation of the efficacy of anti-platelet therapy in patients at risk of thrombosis. Sodium citrate is the most widely used anticoagulant for hemostasis investigations. However, some doubt exists over the suitability of sodium citrate in platelet function testing. Hirudin has been suggested as a superior replacement. Nevertheless, only 1 study compared citrated and hirudin treated samples with light transmission aggregometry. Therefore, limited evidence exists to conclusively prove the supremacy of hirudin over sodium citrate in light transmission aggregometry. The aim of our study was to compare citrated and hirudin treated samples, collected in commercially available blood collection tubes, using the 5 most common agonists, with light transmission aggregometry. MATERIAL/METHODS: Blood was obtained from 20 healthy volunteers. Platelet counts were performed on platelet-rich plasma. Light transmission aggregometry was performed within 4 h of sample collection using ADP, collagen, arachidonic acid, epinephrine, and ristocetin as agonists. RESULTS: Platelet counts for the respective anticoagulants did not differ significantly. ADP-induced aggregation was comparable between the samples. However, among all the agonists, hirudin-treated platelets had significantly weaker aggregatory responses. CONCLUSIONS: Commercially available sodium citrate should remain the anticoagulant of choice for routine platelet function testing in our setting. However, the time limitation associated with the use of sodium citrate in platelet function testing remains a concern. Thus, alternative anticoagulants should still be explored. International Scientific Literature, Inc. 2017-07-27 /pmc/articles/PMC5543979/ /pubmed/28747619 http://dx.doi.org/10.12659/MSMBR.905246 Text en © Med Sci Monit, 2017 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Human Study
Janse Van Rensburg, Walter J.
van der Merwe, Patricia
Comparison of Commercially Available Blood Collection Tubes Containing Sodium Citrate and Hirudin in Platelet Aggregation Testing
title Comparison of Commercially Available Blood Collection Tubes Containing Sodium Citrate and Hirudin in Platelet Aggregation Testing
title_full Comparison of Commercially Available Blood Collection Tubes Containing Sodium Citrate and Hirudin in Platelet Aggregation Testing
title_fullStr Comparison of Commercially Available Blood Collection Tubes Containing Sodium Citrate and Hirudin in Platelet Aggregation Testing
title_full_unstemmed Comparison of Commercially Available Blood Collection Tubes Containing Sodium Citrate and Hirudin in Platelet Aggregation Testing
title_short Comparison of Commercially Available Blood Collection Tubes Containing Sodium Citrate and Hirudin in Platelet Aggregation Testing
title_sort comparison of commercially available blood collection tubes containing sodium citrate and hirudin in platelet aggregation testing
topic Human Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543979/
https://www.ncbi.nlm.nih.gov/pubmed/28747619
http://dx.doi.org/10.12659/MSMBR.905246
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