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Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke

OBJECTIVE: To investigate the neuroprotective effects of Ginkgolide B (GB) against ischemic stroke-induced injury in vivo and in vitro, and further explore the possible mechanisms concerned. METHODS: Transient middle cerebral artery occlusion (tMCAO) mice and oxygen-glucose deprivation/reoxygenation...

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Autores principales: Wei, Hu, Sun, Tao, Tian, Yanghua, Wang, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Neurosurgical Society 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544371/
https://www.ncbi.nlm.nih.gov/pubmed/28689387
http://dx.doi.org/10.3340/jkns.2016.1010.018
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author Wei, Hu
Sun, Tao
Tian, Yanghua
Wang, Kai
author_facet Wei, Hu
Sun, Tao
Tian, Yanghua
Wang, Kai
author_sort Wei, Hu
collection PubMed
description OBJECTIVE: To investigate the neuroprotective effects of Ginkgolide B (GB) against ischemic stroke-induced injury in vivo and in vitro, and further explore the possible mechanisms concerned. METHODS: Transient middle cerebral artery occlusion (tMCAO) mice and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated N2a cells were used to explore the neuroprotective effects of GB. The expression of brain-derived neurotrophic factor (BDNF) was detected via Western blot and qRT-PCR. RESULTS: GB treatment (4 mg/kg, i. p., bid) significantly reduced neurological deficits, water content, and cerebral infarct volume in tMCAO mice. GB also significantly increased Bcl-2/Bax ratio, reduced the expression of caspase-3, and protected against OGD/R-induced neuronal apoptosis. Meanwhile, GB caused the up-regulation of BDNF protein in vivo and in vitro. CONCLUSION: Our data suggest that GB might protect the brain against ischemic insult partly via modulating BDNF expression.
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spelling pubmed-55443712017-08-09 Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke Wei, Hu Sun, Tao Tian, Yanghua Wang, Kai J Korean Neurosurg Soc Laboratory Investigation OBJECTIVE: To investigate the neuroprotective effects of Ginkgolide B (GB) against ischemic stroke-induced injury in vivo and in vitro, and further explore the possible mechanisms concerned. METHODS: Transient middle cerebral artery occlusion (tMCAO) mice and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated N2a cells were used to explore the neuroprotective effects of GB. The expression of brain-derived neurotrophic factor (BDNF) was detected via Western blot and qRT-PCR. RESULTS: GB treatment (4 mg/kg, i. p., bid) significantly reduced neurological deficits, water content, and cerebral infarct volume in tMCAO mice. GB also significantly increased Bcl-2/Bax ratio, reduced the expression of caspase-3, and protected against OGD/R-induced neuronal apoptosis. Meanwhile, GB caused the up-regulation of BDNF protein in vivo and in vitro. CONCLUSION: Our data suggest that GB might protect the brain against ischemic insult partly via modulating BDNF expression. Korean Neurosurgical Society 2017-07 2017-07-31 /pmc/articles/PMC5544371/ /pubmed/28689387 http://dx.doi.org/10.3340/jkns.2016.1010.018 Text en Copyright © 2017 The Korean Neurosurgical Society This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Laboratory Investigation
Wei, Hu
Sun, Tao
Tian, Yanghua
Wang, Kai
Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke
title Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke
title_full Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke
title_fullStr Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke
title_full_unstemmed Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke
title_short Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke
title_sort ginkgolide b modulates bdnf expression in acute ischemic stroke
topic Laboratory Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544371/
https://www.ncbi.nlm.nih.gov/pubmed/28689387
http://dx.doi.org/10.3340/jkns.2016.1010.018
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