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Neurite elongation is highly correlated with bulk forward translocation of microtubules

During the development of the nervous system and regeneration following injury, microtubules (MTs) are required for neurite elongation. Whether this elongation occurs primarily through tubulin assembly at the tip of the axon, the transport of individual MTs, or because MTs translocate forward in bul...

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Autores principales: Athamneh, Ahmad I. M., He, Yingpei, Lamoureux, Phillip, Fix, Lucas, Suter, Daniel M., Miller, Kyle E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544698/
https://www.ncbi.nlm.nih.gov/pubmed/28779177
http://dx.doi.org/10.1038/s41598-017-07402-6
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author Athamneh, Ahmad I. M.
He, Yingpei
Lamoureux, Phillip
Fix, Lucas
Suter, Daniel M.
Miller, Kyle E.
author_facet Athamneh, Ahmad I. M.
He, Yingpei
Lamoureux, Phillip
Fix, Lucas
Suter, Daniel M.
Miller, Kyle E.
author_sort Athamneh, Ahmad I. M.
collection PubMed
description During the development of the nervous system and regeneration following injury, microtubules (MTs) are required for neurite elongation. Whether this elongation occurs primarily through tubulin assembly at the tip of the axon, the transport of individual MTs, or because MTs translocate forward in bulk is unclear. Using fluorescent speckle microscopy (FSM), differential interference contrast (DIC), and phase contrast microscopy, we tracked the movement of MTs, phase dense material, and docked mitochondria in chick sensory and Aplysia bag cell neurons growing rapidly on physiological substrates. In all cases, we find that MTs and other neuritic components move forward in bulk at a rate that on average matches the velocity of neurite elongation. To better understand whether and why MT assembly is required for bulk translocation, we disrupted it with nocodazole. We found this blocked the forward bulk advance of material along the neurite and was paired with a transient increase in axonal tension. This indicates that disruption of MT dynamics interferes with neurite outgrowth, not by disrupting the net assembly of MTs at the growth cone, but rather because it alters the balance of forces that power the bulk forward translocation of MTs.
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spelling pubmed-55446982017-08-07 Neurite elongation is highly correlated with bulk forward translocation of microtubules Athamneh, Ahmad I. M. He, Yingpei Lamoureux, Phillip Fix, Lucas Suter, Daniel M. Miller, Kyle E. Sci Rep Article During the development of the nervous system and regeneration following injury, microtubules (MTs) are required for neurite elongation. Whether this elongation occurs primarily through tubulin assembly at the tip of the axon, the transport of individual MTs, or because MTs translocate forward in bulk is unclear. Using fluorescent speckle microscopy (FSM), differential interference contrast (DIC), and phase contrast microscopy, we tracked the movement of MTs, phase dense material, and docked mitochondria in chick sensory and Aplysia bag cell neurons growing rapidly on physiological substrates. In all cases, we find that MTs and other neuritic components move forward in bulk at a rate that on average matches the velocity of neurite elongation. To better understand whether and why MT assembly is required for bulk translocation, we disrupted it with nocodazole. We found this blocked the forward bulk advance of material along the neurite and was paired with a transient increase in axonal tension. This indicates that disruption of MT dynamics interferes with neurite outgrowth, not by disrupting the net assembly of MTs at the growth cone, but rather because it alters the balance of forces that power the bulk forward translocation of MTs. Nature Publishing Group UK 2017-08-04 /pmc/articles/PMC5544698/ /pubmed/28779177 http://dx.doi.org/10.1038/s41598-017-07402-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Athamneh, Ahmad I. M.
He, Yingpei
Lamoureux, Phillip
Fix, Lucas
Suter, Daniel M.
Miller, Kyle E.
Neurite elongation is highly correlated with bulk forward translocation of microtubules
title Neurite elongation is highly correlated with bulk forward translocation of microtubules
title_full Neurite elongation is highly correlated with bulk forward translocation of microtubules
title_fullStr Neurite elongation is highly correlated with bulk forward translocation of microtubules
title_full_unstemmed Neurite elongation is highly correlated with bulk forward translocation of microtubules
title_short Neurite elongation is highly correlated with bulk forward translocation of microtubules
title_sort neurite elongation is highly correlated with bulk forward translocation of microtubules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544698/
https://www.ncbi.nlm.nih.gov/pubmed/28779177
http://dx.doi.org/10.1038/s41598-017-07402-6
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