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Dietary perturbations alter the ecological significance of ingested Lactobacillus plantarum in the digestive tract

Host diet is a major determinant of the composition and function of the intestinal microbiome. Less understood is the importance of diet on ingested strains with probiotic significance. We investigated the population dynamics of exogenous Lactobacillus plantarum and its interactions with intestinal...

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Autores principales: Yin, Xiaochen, Lee, Bokyung, Zaragoza, Jose, Marco, Maria L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544775/
https://www.ncbi.nlm.nih.gov/pubmed/28779118
http://dx.doi.org/10.1038/s41598-017-07428-w
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author Yin, Xiaochen
Lee, Bokyung
Zaragoza, Jose
Marco, Maria L.
author_facet Yin, Xiaochen
Lee, Bokyung
Zaragoza, Jose
Marco, Maria L.
author_sort Yin, Xiaochen
collection PubMed
description Host diet is a major determinant of the composition and function of the intestinal microbiome. Less understood is the importance of diet on ingested strains with probiotic significance. We investigated the population dynamics of exogenous Lactobacillus plantarum and its interactions with intestinal bacteria in mice undergoing switches between high-fat, high-sugar (HFHSD) and low-fat, plant-polysaccharide rich (LFPPD) diets. The survival and persistence of ingested L. plantarum WCFS1 was significantly improved during mouse consumption of HFHSD and was negatively associated with the numbers of indigenous Lactobacillus species. Diet also rapidly changed the composition of the indigenous microbiota, but with some taxa differentially affected between HFHSD periods. L. plantarum was not integrated into indigenous bacterial community networks according to co-occurrence patterns but still conferred distinct effects on bacterial species diversity and microbiota stability largely in a diet-dependent manner. Metagenome predictions supported the premise that L. plantarum dampens the effects of diet on the microbiome. This strain also consistently altered the predicted genetic content in the distal gut by enriching for genes encoding glyosyltransferases and bile salt hydrolases. Our findings demonstrate the interactions between ingested, transient probiotic bacteria and intestinal bacterial communities and how they can differ depending on host diet.
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spelling pubmed-55447752017-08-09 Dietary perturbations alter the ecological significance of ingested Lactobacillus plantarum in the digestive tract Yin, Xiaochen Lee, Bokyung Zaragoza, Jose Marco, Maria L. Sci Rep Article Host diet is a major determinant of the composition and function of the intestinal microbiome. Less understood is the importance of diet on ingested strains with probiotic significance. We investigated the population dynamics of exogenous Lactobacillus plantarum and its interactions with intestinal bacteria in mice undergoing switches between high-fat, high-sugar (HFHSD) and low-fat, plant-polysaccharide rich (LFPPD) diets. The survival and persistence of ingested L. plantarum WCFS1 was significantly improved during mouse consumption of HFHSD and was negatively associated with the numbers of indigenous Lactobacillus species. Diet also rapidly changed the composition of the indigenous microbiota, but with some taxa differentially affected between HFHSD periods. L. plantarum was not integrated into indigenous bacterial community networks according to co-occurrence patterns but still conferred distinct effects on bacterial species diversity and microbiota stability largely in a diet-dependent manner. Metagenome predictions supported the premise that L. plantarum dampens the effects of diet on the microbiome. This strain also consistently altered the predicted genetic content in the distal gut by enriching for genes encoding glyosyltransferases and bile salt hydrolases. Our findings demonstrate the interactions between ingested, transient probiotic bacteria and intestinal bacterial communities and how they can differ depending on host diet. Nature Publishing Group UK 2017-08-04 /pmc/articles/PMC5544775/ /pubmed/28779118 http://dx.doi.org/10.1038/s41598-017-07428-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yin, Xiaochen
Lee, Bokyung
Zaragoza, Jose
Marco, Maria L.
Dietary perturbations alter the ecological significance of ingested Lactobacillus plantarum in the digestive tract
title Dietary perturbations alter the ecological significance of ingested Lactobacillus plantarum in the digestive tract
title_full Dietary perturbations alter the ecological significance of ingested Lactobacillus plantarum in the digestive tract
title_fullStr Dietary perturbations alter the ecological significance of ingested Lactobacillus plantarum in the digestive tract
title_full_unstemmed Dietary perturbations alter the ecological significance of ingested Lactobacillus plantarum in the digestive tract
title_short Dietary perturbations alter the ecological significance of ingested Lactobacillus plantarum in the digestive tract
title_sort dietary perturbations alter the ecological significance of ingested lactobacillus plantarum in the digestive tract
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544775/
https://www.ncbi.nlm.nih.gov/pubmed/28779118
http://dx.doi.org/10.1038/s41598-017-07428-w
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