Effects of silk peptides administration on fat utilization over a whole day in mice

[PURPOSE]: The aim of the present study was to investigate the effects of treatment with silk peptides (SP) on resting energy expenditure over a 24-h period and clarify the molecular mechanism underlying its enhancement of fat utilization in mouse skeletal muscles. [METHODS]: Sixteen male ICR mice (6-weekold) were divided into two groups and treated with distilled water and SP (CON and SP treatment groups, respectively). SP were dissolved in distilled water and administered to the SP group at 800 mg/kg while the CON group was administered distilled water orally daily for 2 weeks. Resting metabolic rate (RMR) was measured before and after SP ingestion for 2 weeks using an open circuit calorimetry system. After the treatments, we collected blood and skeletal muscle samples from the mice. [RESULTS]: Final body weight and body weight gain showed no significant difference in the CON and SP groups. Conversely, food intake was significantly lower in the SP group than it was in the CON group. The comparison of the respiratory exchange rate (RER) at various time points after the 2-week treatment revealed that it was significantly lower in the SP group than it was in the CON group at 9, 15, 16, and 18-22 h (Figure 3 B). The sum of the RER over 24-h was lower in the SP group than it was in the CON group, but the difference was not statistically significant. The protein levels of fatty acid translocase (FAT)/ cluster of differentiation 36 (CD36) were approximately 12% higher in the SP group than they were in the CON group but that of carnitine palmitoyltransferase I (CPTІ) was not significantly different between the two groups. [CONCLUSION]: These results suggest that treatment with SP 800 mg/kg for 2 weeks may promote fat utilization during physical activity, but not the entire day. In addition, SP treatment effectively enhanced FAT/CD36 protein level in skeletal muscle. In future investigations, it would be necessary to elucidate the effects of long-term SP intake on the resting metabolism of both animals and humans.