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Cell surface influenza haemagglutinin can mediate infection by other animal viruses.

We have used filter-grown Madin-Darby canine kidney (MDCK) cells to explore the mechanism by which influenza virus facilitates secondary virus infection. Vesicular stomatitis virus (VSV) and Semliki Forest virus (SFV) infect only through the basolateral surface of these polarized epithelial cells an...

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Detalles Bibliográficos
Autores principales: Fuller, S D, von Bonsdorff, C H, Simons, K
Formato: Texto
Lenguaje:English
Publicado: 1985
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC554532/
https://www.ncbi.nlm.nih.gov/pubmed/2996880
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author Fuller, S D
von Bonsdorff, C H
Simons, K
author_facet Fuller, S D
von Bonsdorff, C H
Simons, K
author_sort Fuller, S D
collection PubMed
description We have used filter-grown Madin-Darby canine kidney (MDCK) cells to explore the mechanism by which influenza virus facilitates secondary virus infection. Vesicular stomatitis virus (VSV) and Semliki Forest virus (SFV) infect only through the basolateral surface of these polarized epithelial cells and not through the apical surface. Prior infection with influenza virus rendered the cell susceptible to infection by VSV or SFV through either surface. The presence of both a permissive and a restrictive surface for virus entry in the same cell allowed us to determine how the influenza infection enhanced the subsequent infection of a second virus. Biochemical and morphological evidence showed that influenza haemagglutinin on the apical surface serves as a receptor for the superinfecting virus by binding to its sialic acid-bearing envelope proteins. Influenza virus also facilitates secondary virus infection in non-epithelial cells; baby hamster kidney cells (BHK-21), which are normally resistant to infection by the coronavirus (mouse hepatitis virus MHV-A59), could be infected via the haemagglutinin-sialic acid interaction. Facilitation of secondary virus infection requires only the sialic acid-binding properties of the haemagglutinin since the uncleaved haemagglutinin could also mediate virus entry.
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spelling pubmed-5545322005-04-27 Cell surface influenza haemagglutinin can mediate infection by other animal viruses. Fuller, S D von Bonsdorff, C H Simons, K EMBO J Research Article We have used filter-grown Madin-Darby canine kidney (MDCK) cells to explore the mechanism by which influenza virus facilitates secondary virus infection. Vesicular stomatitis virus (VSV) and Semliki Forest virus (SFV) infect only through the basolateral surface of these polarized epithelial cells and not through the apical surface. Prior infection with influenza virus rendered the cell susceptible to infection by VSV or SFV through either surface. The presence of both a permissive and a restrictive surface for virus entry in the same cell allowed us to determine how the influenza infection enhanced the subsequent infection of a second virus. Biochemical and morphological evidence showed that influenza haemagglutinin on the apical surface serves as a receptor for the superinfecting virus by binding to its sialic acid-bearing envelope proteins. Influenza virus also facilitates secondary virus infection in non-epithelial cells; baby hamster kidney cells (BHK-21), which are normally resistant to infection by the coronavirus (mouse hepatitis virus MHV-A59), could be infected via the haemagglutinin-sialic acid interaction. Facilitation of secondary virus infection requires only the sialic acid-binding properties of the haemagglutinin since the uncleaved haemagglutinin could also mediate virus entry. 1985-10 /pmc/articles/PMC554532/ /pubmed/2996880 Text en
spellingShingle Research Article
Fuller, S D
von Bonsdorff, C H
Simons, K
Cell surface influenza haemagglutinin can mediate infection by other animal viruses.
title Cell surface influenza haemagglutinin can mediate infection by other animal viruses.
title_full Cell surface influenza haemagglutinin can mediate infection by other animal viruses.
title_fullStr Cell surface influenza haemagglutinin can mediate infection by other animal viruses.
title_full_unstemmed Cell surface influenza haemagglutinin can mediate infection by other animal viruses.
title_short Cell surface influenza haemagglutinin can mediate infection by other animal viruses.
title_sort cell surface influenza haemagglutinin can mediate infection by other animal viruses.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC554532/
https://www.ncbi.nlm.nih.gov/pubmed/2996880
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