Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016

We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and a...

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Autores principales: Larsen, Jesper, Sunde, Marianne, Islam, Md Zohorul, Urdahl, Anne Margrete, Barstad, Aina Steihaug, Larsen, Anders Rhod, Grøntvedt, Carl Andreas, Angen, Øystein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2017
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5545765/
https://www.ncbi.nlm.nih.gov/pubmed/28749335
http://dx.doi.org/10.2807/1560-7917.ES.2017.22.28.30573
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author Larsen, Jesper
Sunde, Marianne
Islam, Md Zohorul
Urdahl, Anne Margrete
Barstad, Aina Steihaug
Larsen, Anders Rhod
Grøntvedt, Carl Andreas
Angen, Øystein
author_facet Larsen, Jesper
Sunde, Marianne
Islam, Md Zohorul
Urdahl, Anne Margrete
Barstad, Aina Steihaug
Larsen, Anders Rhod
Grøntvedt, Carl Andreas
Angen, Øystein
author_sort Larsen, Jesper
collection PubMed
description We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.
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spelling pubmed-55457652017-08-24 Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016 Larsen, Jesper Sunde, Marianne Islam, Md Zohorul Urdahl, Anne Margrete Barstad, Aina Steihaug Larsen, Anders Rhod Grøntvedt, Carl Andreas Angen, Øystein Euro Surveill Rapid Communication We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples. European Centre for Disease Prevention and Control (ECDC) 2017-07-13 /pmc/articles/PMC5545765/ /pubmed/28749335 http://dx.doi.org/10.2807/1560-7917.ES.2017.22.28.30573 Text en This article is copyright of The Authors, 2017. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Rapid Communication
Larsen, Jesper
Sunde, Marianne
Islam, Md Zohorul
Urdahl, Anne Margrete
Barstad, Aina Steihaug
Larsen, Anders Rhod
Grøntvedt, Carl Andreas
Angen, Øystein
Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016
title Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016
title_full Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016
title_fullStr Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016
title_full_unstemmed Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016
title_short Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016
title_sort evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant staphylococcus aureus (mrsa), denmark and norway, 2014 to 2016
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5545765/
https://www.ncbi.nlm.nih.gov/pubmed/28749335
http://dx.doi.org/10.2807/1560-7917.ES.2017.22.28.30573
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