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Microfluidic device for primary tumor spheroid isolation

BACKGROUND: Traditional two-dimensional (2-D) monolayer cell culture is vastly different from in vivo physiological conditions, which can lead to inaccurate or insufficient data in areas where response and efficacy within humans are being investigated, such as drug discovery, pathology studies, etc....

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Autores principales: Zhou, Jiaojiao, Su, Jimmy, Fu, Xiaotong, Zheng, Lei, Yin, Zhizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5545869/
https://www.ncbi.nlm.nih.gov/pubmed/28794917
http://dx.doi.org/10.1186/s40164-017-0084-3
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author Zhou, Jiaojiao
Su, Jimmy
Fu, Xiaotong
Zheng, Lei
Yin, Zhizhong
author_facet Zhou, Jiaojiao
Su, Jimmy
Fu, Xiaotong
Zheng, Lei
Yin, Zhizhong
author_sort Zhou, Jiaojiao
collection PubMed
description BACKGROUND: Traditional two-dimensional (2-D) monolayer cell culture is vastly different from in vivo physiological conditions, which can lead to inaccurate or insufficient data in areas where response and efficacy within humans are being investigated, such as drug discovery, pathology studies, etc. Misleading results arise from two main disadvantages of monolayer cell culture. First, after several passages, cell lines lose many features from their original in vivo state. Second, the morphology of cells cultured in a monolayer is much different from the cell morphology in three-dimensional (3-D) in vivo conditions, thus resulting in altered cellular function. Three-dimensional multi-cellular spheroids, on the other hand, are a better representation of in vivo physiological conditions while still retaining many of the in vitro cell culture advantages. Primary spheroids freshly isolated from tissue samples are especially ideal for cell-based assays by avoiding the two problems of 2-D monolayer cell culture. METHODS: In this paper, we report a microfluidic device for primary tumor spheroid isolation. Pancreatic tumor samples from mice were used in the experiments. RESULTS: We successfully isolated primary tumor spheroids from the pancreatic tumor samples and were able to maintain the spheroids in culture for up to two weeks. CONCLUSIONS: This novel microfluidic device may promote and advance the isolation of primary tumor spheroids for future drug testing and interrogation of tumor characteristics.
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spelling pubmed-55458692017-08-09 Microfluidic device for primary tumor spheroid isolation Zhou, Jiaojiao Su, Jimmy Fu, Xiaotong Zheng, Lei Yin, Zhizhong Exp Hematol Oncol Short Report BACKGROUND: Traditional two-dimensional (2-D) monolayer cell culture is vastly different from in vivo physiological conditions, which can lead to inaccurate or insufficient data in areas where response and efficacy within humans are being investigated, such as drug discovery, pathology studies, etc. Misleading results arise from two main disadvantages of monolayer cell culture. First, after several passages, cell lines lose many features from their original in vivo state. Second, the morphology of cells cultured in a monolayer is much different from the cell morphology in three-dimensional (3-D) in vivo conditions, thus resulting in altered cellular function. Three-dimensional multi-cellular spheroids, on the other hand, are a better representation of in vivo physiological conditions while still retaining many of the in vitro cell culture advantages. Primary spheroids freshly isolated from tissue samples are especially ideal for cell-based assays by avoiding the two problems of 2-D monolayer cell culture. METHODS: In this paper, we report a microfluidic device for primary tumor spheroid isolation. Pancreatic tumor samples from mice were used in the experiments. RESULTS: We successfully isolated primary tumor spheroids from the pancreatic tumor samples and were able to maintain the spheroids in culture for up to two weeks. CONCLUSIONS: This novel microfluidic device may promote and advance the isolation of primary tumor spheroids for future drug testing and interrogation of tumor characteristics. BioMed Central 2017-08-07 /pmc/articles/PMC5545869/ /pubmed/28794917 http://dx.doi.org/10.1186/s40164-017-0084-3 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Zhou, Jiaojiao
Su, Jimmy
Fu, Xiaotong
Zheng, Lei
Yin, Zhizhong
Microfluidic device for primary tumor spheroid isolation
title Microfluidic device for primary tumor spheroid isolation
title_full Microfluidic device for primary tumor spheroid isolation
title_fullStr Microfluidic device for primary tumor spheroid isolation
title_full_unstemmed Microfluidic device for primary tumor spheroid isolation
title_short Microfluidic device for primary tumor spheroid isolation
title_sort microfluidic device for primary tumor spheroid isolation
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5545869/
https://www.ncbi.nlm.nih.gov/pubmed/28794917
http://dx.doi.org/10.1186/s40164-017-0084-3
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