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Oxidative Biochemistry Disbalance and Changes on Proteomic Profile in Salivary Glands of Rats Induced by Chronic Exposure to Methylmercury

Methylmercury (MeHg) is one of the most toxic mercury species, which can cause many systemic damages, but little is known about its effect in the salivary glands. This study aimed to analyze the mercury levels, oxidative stress, and proteomic profile in parotid, submandibular, and sublingual salivar...

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Autores principales: Bittencourt, Leonardo Oliveira, Puty, Bruna, Charone, Senda, Aragão, Walessa Alana Bragança, Farias-Junior, Paulo Mecenas, Silva, Marcia Cristina Freitas, Crespo-Lopez, Maria Elena, Leite, Aline de Lima, Buzalaf, Marilia Afonso Rabelo, Lima, Rafael Rodrigues
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546058/
https://www.ncbi.nlm.nih.gov/pubmed/28811865
http://dx.doi.org/10.1155/2017/5653291
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author Bittencourt, Leonardo Oliveira
Puty, Bruna
Charone, Senda
Aragão, Walessa Alana Bragança
Farias-Junior, Paulo Mecenas
Silva, Marcia Cristina Freitas
Crespo-Lopez, Maria Elena
Leite, Aline de Lima
Buzalaf, Marilia Afonso Rabelo
Lima, Rafael Rodrigues
author_facet Bittencourt, Leonardo Oliveira
Puty, Bruna
Charone, Senda
Aragão, Walessa Alana Bragança
Farias-Junior, Paulo Mecenas
Silva, Marcia Cristina Freitas
Crespo-Lopez, Maria Elena
Leite, Aline de Lima
Buzalaf, Marilia Afonso Rabelo
Lima, Rafael Rodrigues
author_sort Bittencourt, Leonardo Oliveira
collection PubMed
description Methylmercury (MeHg) is one of the most toxic mercury species, which can cause many systemic damages, but little is known about its effect in the salivary glands. This study aimed to analyze the mercury levels, oxidative stress, and proteomic profile in parotid, submandibular, and sublingual salivary glands of rats, after chronic MeHg intoxication. Two groups of twenty male Wistar rats (90 days of age) were used on the experiment. MeHg group was intoxicated by intragastric gavage with MeHg at a dose of 0.04 mg/kg/day for 60 days, while the control group received only oil. After the period of intoxication, the glands were collected for evaluation of total mercury levels, proteomic profile, and oxidative balance by analyzing the antioxidant capacity against peroxyl radicals (ACAP), lipid peroxidation (LPO), and nitrite levels. Our results have showed that mercury levels were significant in all three glands compared to the respective control. It also showed lower levels of ACAP, as well as higher LPO and nitrite levels. The proteomic profile presented impairments on structural components of cytoskeleton, metabolic pathways, and oxidative biochemistry. Thus, the exposure to MeHg was able to generate oxidative stress that could be associated with changes in the proteomic profile of salivary glands.
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spelling pubmed-55460582017-08-15 Oxidative Biochemistry Disbalance and Changes on Proteomic Profile in Salivary Glands of Rats Induced by Chronic Exposure to Methylmercury Bittencourt, Leonardo Oliveira Puty, Bruna Charone, Senda Aragão, Walessa Alana Bragança Farias-Junior, Paulo Mecenas Silva, Marcia Cristina Freitas Crespo-Lopez, Maria Elena Leite, Aline de Lima Buzalaf, Marilia Afonso Rabelo Lima, Rafael Rodrigues Oxid Med Cell Longev Research Article Methylmercury (MeHg) is one of the most toxic mercury species, which can cause many systemic damages, but little is known about its effect in the salivary glands. This study aimed to analyze the mercury levels, oxidative stress, and proteomic profile in parotid, submandibular, and sublingual salivary glands of rats, after chronic MeHg intoxication. Two groups of twenty male Wistar rats (90 days of age) were used on the experiment. MeHg group was intoxicated by intragastric gavage with MeHg at a dose of 0.04 mg/kg/day for 60 days, while the control group received only oil. After the period of intoxication, the glands were collected for evaluation of total mercury levels, proteomic profile, and oxidative balance by analyzing the antioxidant capacity against peroxyl radicals (ACAP), lipid peroxidation (LPO), and nitrite levels. Our results have showed that mercury levels were significant in all three glands compared to the respective control. It also showed lower levels of ACAP, as well as higher LPO and nitrite levels. The proteomic profile presented impairments on structural components of cytoskeleton, metabolic pathways, and oxidative biochemistry. Thus, the exposure to MeHg was able to generate oxidative stress that could be associated with changes in the proteomic profile of salivary glands. Hindawi 2017 2017-07-24 /pmc/articles/PMC5546058/ /pubmed/28811865 http://dx.doi.org/10.1155/2017/5653291 Text en Copyright © 2017 Leonardo Oliveira Bittencourt et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bittencourt, Leonardo Oliveira
Puty, Bruna
Charone, Senda
Aragão, Walessa Alana Bragança
Farias-Junior, Paulo Mecenas
Silva, Marcia Cristina Freitas
Crespo-Lopez, Maria Elena
Leite, Aline de Lima
Buzalaf, Marilia Afonso Rabelo
Lima, Rafael Rodrigues
Oxidative Biochemistry Disbalance and Changes on Proteomic Profile in Salivary Glands of Rats Induced by Chronic Exposure to Methylmercury
title Oxidative Biochemistry Disbalance and Changes on Proteomic Profile in Salivary Glands of Rats Induced by Chronic Exposure to Methylmercury
title_full Oxidative Biochemistry Disbalance and Changes on Proteomic Profile in Salivary Glands of Rats Induced by Chronic Exposure to Methylmercury
title_fullStr Oxidative Biochemistry Disbalance and Changes on Proteomic Profile in Salivary Glands of Rats Induced by Chronic Exposure to Methylmercury
title_full_unstemmed Oxidative Biochemistry Disbalance and Changes on Proteomic Profile in Salivary Glands of Rats Induced by Chronic Exposure to Methylmercury
title_short Oxidative Biochemistry Disbalance and Changes on Proteomic Profile in Salivary Glands of Rats Induced by Chronic Exposure to Methylmercury
title_sort oxidative biochemistry disbalance and changes on proteomic profile in salivary glands of rats induced by chronic exposure to methylmercury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546058/
https://www.ncbi.nlm.nih.gov/pubmed/28811865
http://dx.doi.org/10.1155/2017/5653291
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