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DNA Remodeling by Strict Partial Endoreplication in Orchids, an Original Process in the Plant Kingdom

DNA remodeling during endoreplication appears to be a strong developmental characteristic in orchids. In this study, we analyzed DNA content and nuclei in 41 species of orchids to further map the genome evolution in this plant family. We demonstrate that the DNA remodeling observed in 36 out of 41 o...

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Autores principales: Brown, Spencer C., Bourge, Mickaël, Maunoury, Nicolas, Wong, Maurice, Wolfe Bianchi, Michele, Lepers-Andrzejewski, Sandra, Besse, Pascale, Siljak-Yakovlev, Sonja, Dron, Michel, Satiat-Jeunemaître, Béatrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546068/
https://www.ncbi.nlm.nih.gov/pubmed/28419219
http://dx.doi.org/10.1093/gbe/evx063
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author Brown, Spencer C.
Bourge, Mickaël
Maunoury, Nicolas
Wong, Maurice
Wolfe Bianchi, Michele
Lepers-Andrzejewski, Sandra
Besse, Pascale
Siljak-Yakovlev, Sonja
Dron, Michel
Satiat-Jeunemaître, Béatrice
author_facet Brown, Spencer C.
Bourge, Mickaël
Maunoury, Nicolas
Wong, Maurice
Wolfe Bianchi, Michele
Lepers-Andrzejewski, Sandra
Besse, Pascale
Siljak-Yakovlev, Sonja
Dron, Michel
Satiat-Jeunemaître, Béatrice
author_sort Brown, Spencer C.
collection PubMed
description DNA remodeling during endoreplication appears to be a strong developmental characteristic in orchids. In this study, we analyzed DNA content and nuclei in 41 species of orchids to further map the genome evolution in this plant family. We demonstrate that the DNA remodeling observed in 36 out of 41 orchids studied corresponds to strict partial endoreplication. Such process is developmentally regulated in each wild species studied. Cytometry data analyses allowed us to propose a model where nuclear states 2C, 4E, 8E, etc. form a series comprising a fixed proportion, the euploid genome 2C, plus 2–32 additional copies of a complementary part of the genome. The fixed proportion ranged from 89% of the genome in Vanilla mexicana down to 19% in V. pompona, the lowest value for all 148 orchids reported. Insterspecific hybridization did not suppress this phenomenon. Interestingly, this process was not observed in mass-produced epiphytes. Nucleolar volumes grow with the number of endocopies present, coherent with high transcription activity in endoreplicated nuclei. Our analyses suggest species-specific chromatin rearrangement. Towards understanding endoreplication, V. planifolia constitutes a tractable system for isolating the genomic sequences that confer an advantage via endoreplication from those that apparently suffice at diploid level.
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spelling pubmed-55460682017-08-24 DNA Remodeling by Strict Partial Endoreplication in Orchids, an Original Process in the Plant Kingdom Brown, Spencer C. Bourge, Mickaël Maunoury, Nicolas Wong, Maurice Wolfe Bianchi, Michele Lepers-Andrzejewski, Sandra Besse, Pascale Siljak-Yakovlev, Sonja Dron, Michel Satiat-Jeunemaître, Béatrice Genome Biol Evol Research Article DNA remodeling during endoreplication appears to be a strong developmental characteristic in orchids. In this study, we analyzed DNA content and nuclei in 41 species of orchids to further map the genome evolution in this plant family. We demonstrate that the DNA remodeling observed in 36 out of 41 orchids studied corresponds to strict partial endoreplication. Such process is developmentally regulated in each wild species studied. Cytometry data analyses allowed us to propose a model where nuclear states 2C, 4E, 8E, etc. form a series comprising a fixed proportion, the euploid genome 2C, plus 2–32 additional copies of a complementary part of the genome. The fixed proportion ranged from 89% of the genome in Vanilla mexicana down to 19% in V. pompona, the lowest value for all 148 orchids reported. Insterspecific hybridization did not suppress this phenomenon. Interestingly, this process was not observed in mass-produced epiphytes. Nucleolar volumes grow with the number of endocopies present, coherent with high transcription activity in endoreplicated nuclei. Our analyses suggest species-specific chromatin rearrangement. Towards understanding endoreplication, V. planifolia constitutes a tractable system for isolating the genomic sequences that confer an advantage via endoreplication from those that apparently suffice at diploid level. Oxford University Press 2017-04-01 /pmc/articles/PMC5546068/ /pubmed/28419219 http://dx.doi.org/10.1093/gbe/evx063 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Brown, Spencer C.
Bourge, Mickaël
Maunoury, Nicolas
Wong, Maurice
Wolfe Bianchi, Michele
Lepers-Andrzejewski, Sandra
Besse, Pascale
Siljak-Yakovlev, Sonja
Dron, Michel
Satiat-Jeunemaître, Béatrice
DNA Remodeling by Strict Partial Endoreplication in Orchids, an Original Process in the Plant Kingdom
title DNA Remodeling by Strict Partial Endoreplication in Orchids, an Original Process in the Plant Kingdom
title_full DNA Remodeling by Strict Partial Endoreplication in Orchids, an Original Process in the Plant Kingdom
title_fullStr DNA Remodeling by Strict Partial Endoreplication in Orchids, an Original Process in the Plant Kingdom
title_full_unstemmed DNA Remodeling by Strict Partial Endoreplication in Orchids, an Original Process in the Plant Kingdom
title_short DNA Remodeling by Strict Partial Endoreplication in Orchids, an Original Process in the Plant Kingdom
title_sort dna remodeling by strict partial endoreplication in orchids, an original process in the plant kingdom
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546068/
https://www.ncbi.nlm.nih.gov/pubmed/28419219
http://dx.doi.org/10.1093/gbe/evx063
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