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Novel microsatellite development and characterization for Phacelia formosula (Hydrophyllaceae)(1)
PREMISE OF THE STUDY: Microsatellite primers were developed to characterize genetic diversity and structuring in the genus Phacelia (Hydrophyllaceae) and to further conservation efforts for P. formosula. METHODS AND RESULTS: Fifteen novel microsatellite primers were developed for P. formosula. These...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546168/ https://www.ncbi.nlm.nih.gov/pubmed/28791208 http://dx.doi.org/10.3732/apps.1700030 |
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author | Riser, James P. Schwabe, Anna L. Neale, Jennifer Ramp |
author_facet | Riser, James P. Schwabe, Anna L. Neale, Jennifer Ramp |
author_sort | Riser, James P. |
collection | PubMed |
description | PREMISE OF THE STUDY: Microsatellite primers were developed to characterize genetic diversity and structuring in the genus Phacelia (Hydrophyllaceae) and to further conservation efforts for P. formosula. METHODS AND RESULTS: Fifteen novel microsatellite primers were developed for P. formosula. These were characterized for genetic variation in three separate P. formosula populations. Two to nine alleles were found per locus. Overall observed heterozygosity and expected heterozygosity ranged from 0.000 to 0.800 and 0.000 to 0.840, respectively. Additionally, these loci were successfully amplified and showed polymorphism in P. gina-glenneae and a potential new Phacelia species. CONCLUSIONS: These microsatellite markers will be useful in assessing genetic diversity, structuring, and gene flow within and among populations of the rare P. formosula, in addition to related Phacelia species. These markers will provide important genetic data needed for appropriate conservation and management of these rare plants. |
format | Online Article Text |
id | pubmed-5546168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-55461682017-08-08 Novel microsatellite development and characterization for Phacelia formosula (Hydrophyllaceae)(1) Riser, James P. Schwabe, Anna L. Neale, Jennifer Ramp Appl Plant Sci Primer Note PREMISE OF THE STUDY: Microsatellite primers were developed to characterize genetic diversity and structuring in the genus Phacelia (Hydrophyllaceae) and to further conservation efforts for P. formosula. METHODS AND RESULTS: Fifteen novel microsatellite primers were developed for P. formosula. These were characterized for genetic variation in three separate P. formosula populations. Two to nine alleles were found per locus. Overall observed heterozygosity and expected heterozygosity ranged from 0.000 to 0.800 and 0.000 to 0.840, respectively. Additionally, these loci were successfully amplified and showed polymorphism in P. gina-glenneae and a potential new Phacelia species. CONCLUSIONS: These microsatellite markers will be useful in assessing genetic diversity, structuring, and gene flow within and among populations of the rare P. formosula, in addition to related Phacelia species. These markers will provide important genetic data needed for appropriate conservation and management of these rare plants. Botanical Society of America 2017-07-19 /pmc/articles/PMC5546168/ /pubmed/28791208 http://dx.doi.org/10.3732/apps.1700030 Text en © 2017 Riser et al. Published by the Botanical Society of America https://creativecommons.org/licenses/by-nc-sa/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC-SA 4.0 (https://creativecommons.org/licenses/by-nc-sa/4.0/) ), which permits unrestricted noncommercial use and redistribution provided that the original author and source are credited and the new work is distributed under the same license as the original. |
spellingShingle | Primer Note Riser, James P. Schwabe, Anna L. Neale, Jennifer Ramp Novel microsatellite development and characterization for Phacelia formosula (Hydrophyllaceae)(1) |
title | Novel microsatellite development and characterization for Phacelia formosula (Hydrophyllaceae)(1) |
title_full | Novel microsatellite development and characterization for Phacelia formosula (Hydrophyllaceae)(1) |
title_fullStr | Novel microsatellite development and characterization for Phacelia formosula (Hydrophyllaceae)(1) |
title_full_unstemmed | Novel microsatellite development and characterization for Phacelia formosula (Hydrophyllaceae)(1) |
title_short | Novel microsatellite development and characterization for Phacelia formosula (Hydrophyllaceae)(1) |
title_sort | novel microsatellite development and characterization for phacelia formosula (hydrophyllaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546168/ https://www.ncbi.nlm.nih.gov/pubmed/28791208 http://dx.doi.org/10.3732/apps.1700030 |
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