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Methionine restriction on oxidative stress and immune response in dss-induced colitis mice
A strong correlation exists between inflammatory bowel disease (IBD) and oxidative stress involving alterations of several key signaling pathways. It is known that methionine promotes reactive oxygen species (ROS) production; we therefore hypothesize that a methionine restriction diet would reduce R...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546498/ https://www.ncbi.nlm.nih.gov/pubmed/28562346 http://dx.doi.org/10.18632/oncotarget.17812 |
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author | Liu, Gang Yu, Lei Fang, Jun Hu, Chien-An Andy Yin, Jie Ni, Hengjia Ren, Wenkai Duraipandiyan, Veeramuthu Chen, Shuai Al-Dhabi, Naif Abdullah Yin, Yulong |
author_facet | Liu, Gang Yu, Lei Fang, Jun Hu, Chien-An Andy Yin, Jie Ni, Hengjia Ren, Wenkai Duraipandiyan, Veeramuthu Chen, Shuai Al-Dhabi, Naif Abdullah Yin, Yulong |
author_sort | Liu, Gang |
collection | PubMed |
description | A strong correlation exists between inflammatory bowel disease (IBD) and oxidative stress involving alterations of several key signaling pathways. It is known that methionine promotes reactive oxygen species (ROS) production; we therefore hypothesize that a methionine restriction diet would reduce ROS production, inflammatory responses, and the course of IBD. We generated a murine colitis model by dextran sodium sulfate (DSS) treatment and tested the effects of the methionine restriction diet. Forty-eight mice were randomly divided into four groups of equal size, which included a control (CON) group, an MR (methionine restriction diet) group, a DSS treated group and an MR-DSS treated group. Mice in the first two groups had unrestricted access to water for one week. Mice in the two DSS-treated groups had unrestricted access to 5% DSS solution supplied in the drinking water for the same period. Mice in the CON and DSS groups were given a basal diet, whereas mice in the MR-DSS and MR groups were fed a 0.14% MR diet. We found that DSS reduced daily weight gain, suppressed antioxidant enzyme expression, increased histopathology scores and activated NF-κB and nuclear factor erythroid 2-related factor 2/Kelch-like ECH-associated protein 1 (Nrf2/Keap1) signaling. We also showed that the MR diet upregulated catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities, decreased myeloperoxidase (MPO), TNF-α and IL-1β, and reversed activation of the NF-κB signaling pathway in MR-DSS mice. Taken together, our results imply that the MR diet may be considered as an adjuvant in IBD therapeutics. |
format | Online Article Text |
id | pubmed-5546498 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-55464982017-08-23 Methionine restriction on oxidative stress and immune response in dss-induced colitis mice Liu, Gang Yu, Lei Fang, Jun Hu, Chien-An Andy Yin, Jie Ni, Hengjia Ren, Wenkai Duraipandiyan, Veeramuthu Chen, Shuai Al-Dhabi, Naif Abdullah Yin, Yulong Oncotarget Research Paper A strong correlation exists between inflammatory bowel disease (IBD) and oxidative stress involving alterations of several key signaling pathways. It is known that methionine promotes reactive oxygen species (ROS) production; we therefore hypothesize that a methionine restriction diet would reduce ROS production, inflammatory responses, and the course of IBD. We generated a murine colitis model by dextran sodium sulfate (DSS) treatment and tested the effects of the methionine restriction diet. Forty-eight mice were randomly divided into four groups of equal size, which included a control (CON) group, an MR (methionine restriction diet) group, a DSS treated group and an MR-DSS treated group. Mice in the first two groups had unrestricted access to water for one week. Mice in the two DSS-treated groups had unrestricted access to 5% DSS solution supplied in the drinking water for the same period. Mice in the CON and DSS groups were given a basal diet, whereas mice in the MR-DSS and MR groups were fed a 0.14% MR diet. We found that DSS reduced daily weight gain, suppressed antioxidant enzyme expression, increased histopathology scores and activated NF-κB and nuclear factor erythroid 2-related factor 2/Kelch-like ECH-associated protein 1 (Nrf2/Keap1) signaling. We also showed that the MR diet upregulated catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities, decreased myeloperoxidase (MPO), TNF-α and IL-1β, and reversed activation of the NF-κB signaling pathway in MR-DSS mice. Taken together, our results imply that the MR diet may be considered as an adjuvant in IBD therapeutics. Impact Journals LLC 2017-05-11 /pmc/articles/PMC5546498/ /pubmed/28562346 http://dx.doi.org/10.18632/oncotarget.17812 Text en Copyright: © 2017 Liu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Liu, Gang Yu, Lei Fang, Jun Hu, Chien-An Andy Yin, Jie Ni, Hengjia Ren, Wenkai Duraipandiyan, Veeramuthu Chen, Shuai Al-Dhabi, Naif Abdullah Yin, Yulong Methionine restriction on oxidative stress and immune response in dss-induced colitis mice |
title | Methionine restriction on oxidative stress and immune response in dss-induced colitis mice |
title_full | Methionine restriction on oxidative stress and immune response in dss-induced colitis mice |
title_fullStr | Methionine restriction on oxidative stress and immune response in dss-induced colitis mice |
title_full_unstemmed | Methionine restriction on oxidative stress and immune response in dss-induced colitis mice |
title_short | Methionine restriction on oxidative stress and immune response in dss-induced colitis mice |
title_sort | methionine restriction on oxidative stress and immune response in dss-induced colitis mice |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546498/ https://www.ncbi.nlm.nih.gov/pubmed/28562346 http://dx.doi.org/10.18632/oncotarget.17812 |
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