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Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells

Nanocarriers have the potential to improve the therapeutic index of currently available drugs by improving their efficacy and achieving therapeutic steady-state levels over an extended period. The association of maghemite–rhodium citrate (MRC) nanoparticles (NPs) has the potential to increase specif...

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Autores principales: Chaves, Natalia L, Estrela-Lopis, Irina, Böttner, Julia, Lopes, Cláudio AP, Guido, Bruna C, de Sousa, Aparecido R, Báo, Sônia N
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546771/
https://www.ncbi.nlm.nih.gov/pubmed/28814867
http://dx.doi.org/10.2147/IJN.S141582
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author Chaves, Natalia L
Estrela-Lopis, Irina
Böttner, Julia
Lopes, Cláudio AP
Guido, Bruna C
de Sousa, Aparecido R
Báo, Sônia N
author_facet Chaves, Natalia L
Estrela-Lopis, Irina
Böttner, Julia
Lopes, Cláudio AP
Guido, Bruna C
de Sousa, Aparecido R
Báo, Sônia N
author_sort Chaves, Natalia L
collection PubMed
description Nanocarriers have the potential to improve the therapeutic index of currently available drugs by improving their efficacy and achieving therapeutic steady-state levels over an extended period. The association of maghemite–rhodium citrate (MRC) nanoparticles (NPs) has the potential to increase specificity of the cytotoxic action. However, the interaction of these NPs with cells, their uptake mechanism, and subcellular localization need to be elucidated. This work evaluates the uptake mechanism of MRC NPs in metastatic and nonmetastatic breast cancer-cell models, comparing them to a nontumor cell line. MRC NPs uptake in breast cancer cells was more effective than in normal cells, with regard to both the amount of internalized material and the achievement of more strategic intracellular distribution. Moreover, this process occurred through a clathrin-dependent endocytosis pathway with different basal expression levels of this protein in the cell lines tested.
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spelling pubmed-55467712017-08-16 Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells Chaves, Natalia L Estrela-Lopis, Irina Böttner, Julia Lopes, Cláudio AP Guido, Bruna C de Sousa, Aparecido R Báo, Sônia N Int J Nanomedicine Original Research Nanocarriers have the potential to improve the therapeutic index of currently available drugs by improving their efficacy and achieving therapeutic steady-state levels over an extended period. The association of maghemite–rhodium citrate (MRC) nanoparticles (NPs) has the potential to increase specificity of the cytotoxic action. However, the interaction of these NPs with cells, their uptake mechanism, and subcellular localization need to be elucidated. This work evaluates the uptake mechanism of MRC NPs in metastatic and nonmetastatic breast cancer-cell models, comparing them to a nontumor cell line. MRC NPs uptake in breast cancer cells was more effective than in normal cells, with regard to both the amount of internalized material and the achievement of more strategic intracellular distribution. Moreover, this process occurred through a clathrin-dependent endocytosis pathway with different basal expression levels of this protein in the cell lines tested. Dove Medical Press 2017-08-02 /pmc/articles/PMC5546771/ /pubmed/28814867 http://dx.doi.org/10.2147/IJN.S141582 Text en © 2017 Chaves et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Chaves, Natalia L
Estrela-Lopis, Irina
Böttner, Julia
Lopes, Cláudio AP
Guido, Bruna C
de Sousa, Aparecido R
Báo, Sônia N
Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
title Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
title_full Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
title_fullStr Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
title_full_unstemmed Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
title_short Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
title_sort exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546771/
https://www.ncbi.nlm.nih.gov/pubmed/28814867
http://dx.doi.org/10.2147/IJN.S141582
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