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MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a
MicroRNA (miRNA) is believed to play a crucial role in the cause and treatment of epilepsy by controlling gene expression. However, it is still unclear how miRNA profiles change after multiple prolonged seizures and aggravation of brain injury in chronic epilepsy (CE). To investigate the role of miR...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546815/ https://www.ncbi.nlm.nih.gov/pubmed/28814872 http://dx.doi.org/10.2147/NDT.S141062 |
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author | Liu, Xixia Liao, Yuhan Wang, Xiuxiu Zou, Donghua Luo, Chun Jian, Chongdong Wu, Yuan |
author_facet | Liu, Xixia Liao, Yuhan Wang, Xiuxiu Zou, Donghua Luo, Chun Jian, Chongdong Wu, Yuan |
author_sort | Liu, Xixia |
collection | PubMed |
description | MicroRNA (miRNA) is believed to play a crucial role in the cause and treatment of epilepsy by controlling gene expression. However, it is still unclear how miRNA profiles change after multiple prolonged seizures and aggravation of brain injury in chronic epilepsy (CE). To investigate the role of miRNA in epilepsy, we utilized the CE rat models with pentylenetetrazol (PTZ) and miRNA profiles in the hippocampus. miRNA profiles were characterized using miRNA microarray analysis and were compared with the rats in the sham group, which received 0.9% physiological saline treatment at the same dose. Four up-regulated miRNAs (miR-139–3p, -770–5p, -127–5p, -331–3p) and 5 down-regulated miRNAs (miR-802–5p, -380–5p, -183–5p, -547–5p, -344a/-344a–5p) were found in the CE rats (fold change >1.5, P<0.05). Three of the dysregulated miRNAs were validated by quantitative real-time polymerase chain reaction, which revealed an outcome consistent with the initial results of the miRNA microarray analyses. Then, miR-344a agomir was intracerebroventricularly injected and followed by PTZ induction of CE models to investigate the effect of miR-344a in chronic neocortical epileptogenesis. After miRNA-344a agomir and scramble treatment, results showed a restoration of seizure behavior and a reduction in neuron damage in the cortex in miRNA-334a agomir treated rats. These data suggest that miRNA-344a might have a small modulatory effect on seizure-induced apoptosis signaling pathways in the cortex. |
format | Online Article Text |
id | pubmed-5546815 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-55468152017-08-16 MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a Liu, Xixia Liao, Yuhan Wang, Xiuxiu Zou, Donghua Luo, Chun Jian, Chongdong Wu, Yuan Neuropsychiatr Dis Treat Original Research MicroRNA (miRNA) is believed to play a crucial role in the cause and treatment of epilepsy by controlling gene expression. However, it is still unclear how miRNA profiles change after multiple prolonged seizures and aggravation of brain injury in chronic epilepsy (CE). To investigate the role of miRNA in epilepsy, we utilized the CE rat models with pentylenetetrazol (PTZ) and miRNA profiles in the hippocampus. miRNA profiles were characterized using miRNA microarray analysis and were compared with the rats in the sham group, which received 0.9% physiological saline treatment at the same dose. Four up-regulated miRNAs (miR-139–3p, -770–5p, -127–5p, -331–3p) and 5 down-regulated miRNAs (miR-802–5p, -380–5p, -183–5p, -547–5p, -344a/-344a–5p) were found in the CE rats (fold change >1.5, P<0.05). Three of the dysregulated miRNAs were validated by quantitative real-time polymerase chain reaction, which revealed an outcome consistent with the initial results of the miRNA microarray analyses. Then, miR-344a agomir was intracerebroventricularly injected and followed by PTZ induction of CE models to investigate the effect of miR-344a in chronic neocortical epileptogenesis. After miRNA-344a agomir and scramble treatment, results showed a restoration of seizure behavior and a reduction in neuron damage in the cortex in miRNA-334a agomir treated rats. These data suggest that miRNA-344a might have a small modulatory effect on seizure-induced apoptosis signaling pathways in the cortex. Dove Medical Press 2017-07-31 /pmc/articles/PMC5546815/ /pubmed/28814872 http://dx.doi.org/10.2147/NDT.S141062 Text en © 2017 Liu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Liu, Xixia Liao, Yuhan Wang, Xiuxiu Zou, Donghua Luo, Chun Jian, Chongdong Wu, Yuan MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a |
title | MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a |
title_full | MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a |
title_fullStr | MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a |
title_full_unstemmed | MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a |
title_short | MicroRNA expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting miR-344a |
title_sort | microrna expression profiles in chronic epilepsy rats and neuroprotection from seizures by targeting mir-344a |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546815/ https://www.ncbi.nlm.nih.gov/pubmed/28814872 http://dx.doi.org/10.2147/NDT.S141062 |
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