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A pluripotent stem cell-based model for post-implantation human amniotic sac development

Development of the asymmetric amniotic sac—with the embryonic disc and amniotic ectoderm occupying opposite poles—is a vital milestone during human embryo implantation. Although essential to embryogenesis and pregnancy, amniotic sac development in humans remains poorly understood. Here, we report a...

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Autores principales: Shao, Yue, Taniguchi, Kenichiro, Townshend, Ryan F., Miki, Toshio, Gumucio, Deborah L., Fu, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547056/
https://www.ncbi.nlm.nih.gov/pubmed/28785084
http://dx.doi.org/10.1038/s41467-017-00236-w
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author Shao, Yue
Taniguchi, Kenichiro
Townshend, Ryan F.
Miki, Toshio
Gumucio, Deborah L.
Fu, Jianping
author_facet Shao, Yue
Taniguchi, Kenichiro
Townshend, Ryan F.
Miki, Toshio
Gumucio, Deborah L.
Fu, Jianping
author_sort Shao, Yue
collection PubMed
description Development of the asymmetric amniotic sac—with the embryonic disc and amniotic ectoderm occupying opposite poles—is a vital milestone during human embryo implantation. Although essential to embryogenesis and pregnancy, amniotic sac development in humans remains poorly understood. Here, we report a human pluripotent stem cell (hPSC)-based model, termed the post-implantation amniotic sac embryoid (PASE), that recapitulates multiple post-implantation embryogenic events centered around amniotic sac development. Without maternal or extraembryonic tissues, the PASE self-organizes into an epithelial cyst with an asymmetric amniotic ectoderm-epiblast pattern that resembles the human amniotic sac. Upon further development, the PASE initiates a process that resembles posterior primitive streak development in a SNAI1-dependent manner. Furthermore, we observe asymmetric BMP-SMAD signaling concurrent with PASE development, and establish that BMP-SMAD activation/inhibition modulates stable PASE development. This study reveals a previously unrecognized fate potential of human pluripotent stem cells and provides a platform for advancing human embryology.
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spelling pubmed-55470562017-08-11 A pluripotent stem cell-based model for post-implantation human amniotic sac development Shao, Yue Taniguchi, Kenichiro Townshend, Ryan F. Miki, Toshio Gumucio, Deborah L. Fu, Jianping Nat Commun Article Development of the asymmetric amniotic sac—with the embryonic disc and amniotic ectoderm occupying opposite poles—is a vital milestone during human embryo implantation. Although essential to embryogenesis and pregnancy, amniotic sac development in humans remains poorly understood. Here, we report a human pluripotent stem cell (hPSC)-based model, termed the post-implantation amniotic sac embryoid (PASE), that recapitulates multiple post-implantation embryogenic events centered around amniotic sac development. Without maternal or extraembryonic tissues, the PASE self-organizes into an epithelial cyst with an asymmetric amniotic ectoderm-epiblast pattern that resembles the human amniotic sac. Upon further development, the PASE initiates a process that resembles posterior primitive streak development in a SNAI1-dependent manner. Furthermore, we observe asymmetric BMP-SMAD signaling concurrent with PASE development, and establish that BMP-SMAD activation/inhibition modulates stable PASE development. This study reveals a previously unrecognized fate potential of human pluripotent stem cells and provides a platform for advancing human embryology. Nature Publishing Group UK 2017-08-08 /pmc/articles/PMC5547056/ /pubmed/28785084 http://dx.doi.org/10.1038/s41467-017-00236-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shao, Yue
Taniguchi, Kenichiro
Townshend, Ryan F.
Miki, Toshio
Gumucio, Deborah L.
Fu, Jianping
A pluripotent stem cell-based model for post-implantation human amniotic sac development
title A pluripotent stem cell-based model for post-implantation human amniotic sac development
title_full A pluripotent stem cell-based model for post-implantation human amniotic sac development
title_fullStr A pluripotent stem cell-based model for post-implantation human amniotic sac development
title_full_unstemmed A pluripotent stem cell-based model for post-implantation human amniotic sac development
title_short A pluripotent stem cell-based model for post-implantation human amniotic sac development
title_sort pluripotent stem cell-based model for post-implantation human amniotic sac development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547056/
https://www.ncbi.nlm.nih.gov/pubmed/28785084
http://dx.doi.org/10.1038/s41467-017-00236-w
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