In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses

Ribbons are presynaptic structures that mediate synaptic vesicle release in some sensory cells of the auditory and visual systems. Although composed predominately of the protein Ribeye, very little is known about the structural dynamics of ribbons. Here we describe the in vivo mobility and turnover...

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Autores principales: Graydon, Cole W., Manor, Uri, Kindt, Katie S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547071/
https://www.ncbi.nlm.nih.gov/pubmed/28785118
http://dx.doi.org/10.1038/s41598-017-07940-z
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author Graydon, Cole W.
Manor, Uri
Kindt, Katie S.
author_facet Graydon, Cole W.
Manor, Uri
Kindt, Katie S.
author_sort Graydon, Cole W.
collection PubMed
description Ribbons are presynaptic structures that mediate synaptic vesicle release in some sensory cells of the auditory and visual systems. Although composed predominately of the protein Ribeye, very little is known about the structural dynamics of ribbons. Here we describe the in vivo mobility and turnover of Ribeye at hair cell ribbon synapses by monitoring fluorescence recovery after photobleaching (FRAP) in transgenic zebrafish with GFP-tagged Ribeye. We show that Ribeye can exchange between halves of a ribbon within ~1 minute in a manner that is consistent with a simple diffusion mechanism. In contrast, exchange of Ribeye between other ribbons via the cell’s cytoplasm takes several hours.
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spelling pubmed-55470712017-08-09 In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses Graydon, Cole W. Manor, Uri Kindt, Katie S. Sci Rep Article Ribbons are presynaptic structures that mediate synaptic vesicle release in some sensory cells of the auditory and visual systems. Although composed predominately of the protein Ribeye, very little is known about the structural dynamics of ribbons. Here we describe the in vivo mobility and turnover of Ribeye at hair cell ribbon synapses by monitoring fluorescence recovery after photobleaching (FRAP) in transgenic zebrafish with GFP-tagged Ribeye. We show that Ribeye can exchange between halves of a ribbon within ~1 minute in a manner that is consistent with a simple diffusion mechanism. In contrast, exchange of Ribeye between other ribbons via the cell’s cytoplasm takes several hours. Nature Publishing Group UK 2017-08-07 /pmc/articles/PMC5547071/ /pubmed/28785118 http://dx.doi.org/10.1038/s41598-017-07940-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Graydon, Cole W.
Manor, Uri
Kindt, Katie S.
In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
title In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
title_full In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
title_fullStr In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
title_full_unstemmed In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
title_short In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses
title_sort in vivo ribbon mobility and turnover of ribeye at zebrafish hair cell synapses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547071/
https://www.ncbi.nlm.nih.gov/pubmed/28785118
http://dx.doi.org/10.1038/s41598-017-07940-z
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