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Pretreated Glehnia littoralis Extract Prevents Neuronal Death Following Transient Global Cerebral Ischemia through Increases of Superoxide Dismutase 1 and Brain-derived Neurotrophic Factor Expressions in the Gerbil Hippocampal Cornu Ammonis 1 Area

BACKGROUND: Glehnia littoralis, as a traditional herbal medicine to heal various health ailments in East Asia, displays various therapeutic properties including antioxidant effects. However, neuroprotective effects of G. littoralis against cerebral ischemic insults have not yet been addressed. There...

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Autores principales: Park, Joon Ha, Lee, Tae-Kyeong, Yan, Bing-Chun, Shin, Bich-Na, Ahn, Ji Hyeon, Kim, In Hye, Cho, Jeong Hwi, Lee, Jae-Chul, Hwang, In Koo, Kim, Jong Dai, Hong, Seongkweon, Lee, Young Joo, Won, Moo-Ho, Kang, Il Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547831/
https://www.ncbi.nlm.nih.gov/pubmed/28748852
http://dx.doi.org/10.4103/0366-6999.211554
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author Park, Joon Ha
Lee, Tae-Kyeong
Yan, Bing-Chun
Shin, Bich-Na
Ahn, Ji Hyeon
Kim, In Hye
Cho, Jeong Hwi
Lee, Jae-Chul
Hwang, In Koo
Kim, Jong Dai
Hong, Seongkweon
Lee, Young Joo
Won, Moo-Ho
Kang, Il Jun
author_facet Park, Joon Ha
Lee, Tae-Kyeong
Yan, Bing-Chun
Shin, Bich-Na
Ahn, Ji Hyeon
Kim, In Hye
Cho, Jeong Hwi
Lee, Jae-Chul
Hwang, In Koo
Kim, Jong Dai
Hong, Seongkweon
Lee, Young Joo
Won, Moo-Ho
Kang, Il Jun
author_sort Park, Joon Ha
collection PubMed
description BACKGROUND: Glehnia littoralis, as a traditional herbal medicine to heal various health ailments in East Asia, displays various therapeutic properties including antioxidant effects. However, neuroprotective effects of G. littoralis against cerebral ischemic insults have not yet been addressed. Therefore, in this study, we first examined its neuroprotective effects in the hippocampus using a gerbil model of transient global cerebral ischemia (TGCI). METHODS: Gerbils were subjected to TGCI for 5 min. G. littoralis extract (GLE; 100 and 200 mg/kg) was administrated orally once daily for 7 days before ischemic surgery. Neuroprotection was examined by neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. Gliosis was observed by immunohistochemistry for glial fibrillary acidic protein and ionized calcium-binding adapter molecule 1. For neuroprotective mechanisms, immunohistochemistry for superoxide dismutase (SOD) 1 and brain-derived neurotrophic factor (BDNF) was done. RESULTS: Pretreatment with 200 mg/kg of GLE protected pyramidal neurons in the cornu ammonis 1 (CA1) area from ischemic insult area (F = 29.770, P < 0.05) and significantly inhibited activations of astrocytes (F = 22.959, P < 0.05) and microglia (F = 44.135, P < 0.05) in the ischemic CA1 area. In addition, pretreatment with GLE significantly increased expressions of SOD1 (F = 28.561, P < 0.05) and BDNF (F = 55.298, P < 0.05) in CA1 pyramidal neurons of the sham- and ischemia-operated groups. CONCLUSIONS: Our findings indicate that pretreatment with GLE can protect neurons from ischemic insults, and we suggest that its neuroprotective mechanism may be closely associated with increases of SOD1 and BDNF expressions as well as attenuation of glial activation.
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spelling pubmed-55478312017-08-21 Pretreated Glehnia littoralis Extract Prevents Neuronal Death Following Transient Global Cerebral Ischemia through Increases of Superoxide Dismutase 1 and Brain-derived Neurotrophic Factor Expressions in the Gerbil Hippocampal Cornu Ammonis 1 Area Park, Joon Ha Lee, Tae-Kyeong Yan, Bing-Chun Shin, Bich-Na Ahn, Ji Hyeon Kim, In Hye Cho, Jeong Hwi Lee, Jae-Chul Hwang, In Koo Kim, Jong Dai Hong, Seongkweon Lee, Young Joo Won, Moo-Ho Kang, Il Jun Chin Med J (Engl) Original Article BACKGROUND: Glehnia littoralis, as a traditional herbal medicine to heal various health ailments in East Asia, displays various therapeutic properties including antioxidant effects. However, neuroprotective effects of G. littoralis against cerebral ischemic insults have not yet been addressed. Therefore, in this study, we first examined its neuroprotective effects in the hippocampus using a gerbil model of transient global cerebral ischemia (TGCI). METHODS: Gerbils were subjected to TGCI for 5 min. G. littoralis extract (GLE; 100 and 200 mg/kg) was administrated orally once daily for 7 days before ischemic surgery. Neuroprotection was examined by neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. Gliosis was observed by immunohistochemistry for glial fibrillary acidic protein and ionized calcium-binding adapter molecule 1. For neuroprotective mechanisms, immunohistochemistry for superoxide dismutase (SOD) 1 and brain-derived neurotrophic factor (BDNF) was done. RESULTS: Pretreatment with 200 mg/kg of GLE protected pyramidal neurons in the cornu ammonis 1 (CA1) area from ischemic insult area (F = 29.770, P < 0.05) and significantly inhibited activations of astrocytes (F = 22.959, P < 0.05) and microglia (F = 44.135, P < 0.05) in the ischemic CA1 area. In addition, pretreatment with GLE significantly increased expressions of SOD1 (F = 28.561, P < 0.05) and BDNF (F = 55.298, P < 0.05) in CA1 pyramidal neurons of the sham- and ischemia-operated groups. CONCLUSIONS: Our findings indicate that pretreatment with GLE can protect neurons from ischemic insults, and we suggest that its neuroprotective mechanism may be closely associated with increases of SOD1 and BDNF expressions as well as attenuation of glial activation. Medknow Publications & Media Pvt Ltd 2017-08-05 /pmc/articles/PMC5547831/ /pubmed/28748852 http://dx.doi.org/10.4103/0366-6999.211554 Text en Copyright: © 2017 Chinese Medical Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Park, Joon Ha
Lee, Tae-Kyeong
Yan, Bing-Chun
Shin, Bich-Na
Ahn, Ji Hyeon
Kim, In Hye
Cho, Jeong Hwi
Lee, Jae-Chul
Hwang, In Koo
Kim, Jong Dai
Hong, Seongkweon
Lee, Young Joo
Won, Moo-Ho
Kang, Il Jun
Pretreated Glehnia littoralis Extract Prevents Neuronal Death Following Transient Global Cerebral Ischemia through Increases of Superoxide Dismutase 1 and Brain-derived Neurotrophic Factor Expressions in the Gerbil Hippocampal Cornu Ammonis 1 Area
title Pretreated Glehnia littoralis Extract Prevents Neuronal Death Following Transient Global Cerebral Ischemia through Increases of Superoxide Dismutase 1 and Brain-derived Neurotrophic Factor Expressions in the Gerbil Hippocampal Cornu Ammonis 1 Area
title_full Pretreated Glehnia littoralis Extract Prevents Neuronal Death Following Transient Global Cerebral Ischemia through Increases of Superoxide Dismutase 1 and Brain-derived Neurotrophic Factor Expressions in the Gerbil Hippocampal Cornu Ammonis 1 Area
title_fullStr Pretreated Glehnia littoralis Extract Prevents Neuronal Death Following Transient Global Cerebral Ischemia through Increases of Superoxide Dismutase 1 and Brain-derived Neurotrophic Factor Expressions in the Gerbil Hippocampal Cornu Ammonis 1 Area
title_full_unstemmed Pretreated Glehnia littoralis Extract Prevents Neuronal Death Following Transient Global Cerebral Ischemia through Increases of Superoxide Dismutase 1 and Brain-derived Neurotrophic Factor Expressions in the Gerbil Hippocampal Cornu Ammonis 1 Area
title_short Pretreated Glehnia littoralis Extract Prevents Neuronal Death Following Transient Global Cerebral Ischemia through Increases of Superoxide Dismutase 1 and Brain-derived Neurotrophic Factor Expressions in the Gerbil Hippocampal Cornu Ammonis 1 Area
title_sort pretreated glehnia littoralis extract prevents neuronal death following transient global cerebral ischemia through increases of superoxide dismutase 1 and brain-derived neurotrophic factor expressions in the gerbil hippocampal cornu ammonis 1 area
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547831/
https://www.ncbi.nlm.nih.gov/pubmed/28748852
http://dx.doi.org/10.4103/0366-6999.211554
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