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Variant SNPs at the microRNA complementary site in the B7-H1 3′-untranslated region increase the risk of non-small cell lung cancer

Single nucleotide polymorphisms (SNPs) in microRNA-binding sites located in the 3′-untranslated region (UTR) of target genes can have an effect on the interaction of microRNA-mediated regulation, which results in changes in the expression levels of target genes ultimately associated with cancer risk...

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Autores principales: Du, Wenwen, Zhu, Jianjie, Chen, Yanbin, Zeng, Yuanyuan, Shen, Dan, Zhang, Nan, Ning, Weiwei, Liu, Zeyi, Huang, Jian-An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547930/
https://www.ncbi.nlm.nih.gov/pubmed/28677815
http://dx.doi.org/10.3892/mmr.2017.6902
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author Du, Wenwen
Zhu, Jianjie
Chen, Yanbin
Zeng, Yuanyuan
Shen, Dan
Zhang, Nan
Ning, Weiwei
Liu, Zeyi
Huang, Jian-An
author_facet Du, Wenwen
Zhu, Jianjie
Chen, Yanbin
Zeng, Yuanyuan
Shen, Dan
Zhang, Nan
Ning, Weiwei
Liu, Zeyi
Huang, Jian-An
author_sort Du, Wenwen
collection PubMed
description Single nucleotide polymorphisms (SNPs) in microRNA-binding sites located in the 3′-untranslated region (UTR) of target genes can have an effect on the interaction of microRNA-mediated regulation, which results in changes in the expression levels of target genes ultimately associated with cancer risk and patient prognosis. However, the role of SNPs at the 3′-UTR of B7-H1 in the susceptibility of non-small cell lung cancer (NSCLC) remains to be fully elucidated. In the present study, SNPs with a minor allele frequency >10%, which were located at the microRNA complementary site in the PD-L1 3′-UTR, were selected via bioinformatic prediction using Ensembl and miRanda 2010. A total of three SNPs were selected, s2297136, rs4143815 and rs4742098, in the 3′-UTR of B7-H1. The rs2297136 and rs4742098 SNPs exhibited significant differences between 320 patients with NSCLC and 199 healthy individuals, respectively (P<0.001 and P=0.007). For the rs2297136 SNP, the AG genotype was significantly associated with evaluation of the risk of NSCLC, compared the AA genotype [odds ratio (OR)=2.287; 95% confidence interval (95% CI)=1.558–3.358]. Similarly, for the rs4742098 SNP, the AG genotype differed from the AA genotype on evaluation of the risk of NSCLC (OR=1.599; 95% CI=1.027–2.488). Dual-luciferase reporter assays showed that rs2297136 and rs4742098 in the B7-H1 3′-UTR contributed to the occurrence of NSCLC through disrupting the interaction between miR-296-5p, miR-138 and B7-H1 mRNA. These results indicated that genetic polymorphisms affecting the expression of B7-H1 modified cancer susceptibility.
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spelling pubmed-55479302017-10-24 Variant SNPs at the microRNA complementary site in the B7-H1 3′-untranslated region increase the risk of non-small cell lung cancer Du, Wenwen Zhu, Jianjie Chen, Yanbin Zeng, Yuanyuan Shen, Dan Zhang, Nan Ning, Weiwei Liu, Zeyi Huang, Jian-An Mol Med Rep Articles Single nucleotide polymorphisms (SNPs) in microRNA-binding sites located in the 3′-untranslated region (UTR) of target genes can have an effect on the interaction of microRNA-mediated regulation, which results in changes in the expression levels of target genes ultimately associated with cancer risk and patient prognosis. However, the role of SNPs at the 3′-UTR of B7-H1 in the susceptibility of non-small cell lung cancer (NSCLC) remains to be fully elucidated. In the present study, SNPs with a minor allele frequency >10%, which were located at the microRNA complementary site in the PD-L1 3′-UTR, were selected via bioinformatic prediction using Ensembl and miRanda 2010. A total of three SNPs were selected, s2297136, rs4143815 and rs4742098, in the 3′-UTR of B7-H1. The rs2297136 and rs4742098 SNPs exhibited significant differences between 320 patients with NSCLC and 199 healthy individuals, respectively (P<0.001 and P=0.007). For the rs2297136 SNP, the AG genotype was significantly associated with evaluation of the risk of NSCLC, compared the AA genotype [odds ratio (OR)=2.287; 95% confidence interval (95% CI)=1.558–3.358]. Similarly, for the rs4742098 SNP, the AG genotype differed from the AA genotype on evaluation of the risk of NSCLC (OR=1.599; 95% CI=1.027–2.488). Dual-luciferase reporter assays showed that rs2297136 and rs4742098 in the B7-H1 3′-UTR contributed to the occurrence of NSCLC through disrupting the interaction between miR-296-5p, miR-138 and B7-H1 mRNA. These results indicated that genetic polymorphisms affecting the expression of B7-H1 modified cancer susceptibility. D.A. Spandidos 2017-09 2017-06-30 /pmc/articles/PMC5547930/ /pubmed/28677815 http://dx.doi.org/10.3892/mmr.2017.6902 Text en Copyright: © Du et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Du, Wenwen
Zhu, Jianjie
Chen, Yanbin
Zeng, Yuanyuan
Shen, Dan
Zhang, Nan
Ning, Weiwei
Liu, Zeyi
Huang, Jian-An
Variant SNPs at the microRNA complementary site in the B7-H1 3′-untranslated region increase the risk of non-small cell lung cancer
title Variant SNPs at the microRNA complementary site in the B7-H1 3′-untranslated region increase the risk of non-small cell lung cancer
title_full Variant SNPs at the microRNA complementary site in the B7-H1 3′-untranslated region increase the risk of non-small cell lung cancer
title_fullStr Variant SNPs at the microRNA complementary site in the B7-H1 3′-untranslated region increase the risk of non-small cell lung cancer
title_full_unstemmed Variant SNPs at the microRNA complementary site in the B7-H1 3′-untranslated region increase the risk of non-small cell lung cancer
title_short Variant SNPs at the microRNA complementary site in the B7-H1 3′-untranslated region increase the risk of non-small cell lung cancer
title_sort variant snps at the microrna complementary site in the b7-h1 3′-untranslated region increase the risk of non-small cell lung cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547930/
https://www.ncbi.nlm.nih.gov/pubmed/28677815
http://dx.doi.org/10.3892/mmr.2017.6902
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