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Serum biomarker analysis in patients with recurrent spontaneous abortion

Recurrent spontaneous abortion (RSA) occurs in 1–5% of parturients. The sustained therapy and research for RSA is expensive, which is a serious issue faced by both patients and doctors. The aim of the present study was to detect protein expression profiles in the serum of RSA patients and healthy co...

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Autores principales: Wu, Ying, He, Junqin, Guo, Chunyu, Zhang, Ying, Yang, Wei, Xin, Mingwei, Liang, Xinyun, Yin, Xiaodan, Wang, Jingshang, Liu, Yanfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547932/
https://www.ncbi.nlm.nih.gov/pubmed/28677727
http://dx.doi.org/10.3892/mmr.2017.6890
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author Wu, Ying
He, Junqin
Guo, Chunyu
Zhang, Ying
Yang, Wei
Xin, Mingwei
Liang, Xinyun
Yin, Xiaodan
Wang, Jingshang
Liu, Yanfeng
author_facet Wu, Ying
He, Junqin
Guo, Chunyu
Zhang, Ying
Yang, Wei
Xin, Mingwei
Liang, Xinyun
Yin, Xiaodan
Wang, Jingshang
Liu, Yanfeng
author_sort Wu, Ying
collection PubMed
description Recurrent spontaneous abortion (RSA) occurs in 1–5% of parturients. The sustained therapy and research for RSA is expensive, which is a serious issue faced by both patients and doctors. The aim of the present study was to detect protein expression profiles in the serum of RSA patients and healthy controls, and to identify potential biomarkers for this disease. A 1,000-protein microarray consisting of a combination of Human L-507 and L-493 was used. The microarray data revealed that eight serum protein expression levels were significantly upregulated and 143 proteins were downregulated in RSA patients compared with the healthy controls. ELISA individually validated 5 of these 151 proteins in a larger cohort of patients and control samples, demonstrating a significant decrease in insulin-like growth factor-binding protein-related protein 1 (IFGBP-rp1)/IGFBP-7, Dickkopf-related protein 3 (Dkk3), receptor for advanced glycation end products (RAGE) and angiopoietin-2 levels in patients with RSA. Sensitivity and specificity analyses were calculated by a receiver operating characteristics curve, and were revealed to be 0.881, 0.823, 0.79 and 0.814, with diagnostic cut-off points of 95.44 ng/ml for IFGBP-rp1, 32.84 ng/ml for Dkk3, 147.27 ng/ml for RAGE and 441.40 ng/ml for angiopoietin-2. The present study indicated that these four proteins were downregulated in RSA samples and may be useful as biomarkers for the prediction and diagnosis of RSA. Subsequent studies in larger-scale cohorts are required to further validate the diagnostic value of these markers.
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spelling pubmed-55479322017-10-24 Serum biomarker analysis in patients with recurrent spontaneous abortion Wu, Ying He, Junqin Guo, Chunyu Zhang, Ying Yang, Wei Xin, Mingwei Liang, Xinyun Yin, Xiaodan Wang, Jingshang Liu, Yanfeng Mol Med Rep Articles Recurrent spontaneous abortion (RSA) occurs in 1–5% of parturients. The sustained therapy and research for RSA is expensive, which is a serious issue faced by both patients and doctors. The aim of the present study was to detect protein expression profiles in the serum of RSA patients and healthy controls, and to identify potential biomarkers for this disease. A 1,000-protein microarray consisting of a combination of Human L-507 and L-493 was used. The microarray data revealed that eight serum protein expression levels were significantly upregulated and 143 proteins were downregulated in RSA patients compared with the healthy controls. ELISA individually validated 5 of these 151 proteins in a larger cohort of patients and control samples, demonstrating a significant decrease in insulin-like growth factor-binding protein-related protein 1 (IFGBP-rp1)/IGFBP-7, Dickkopf-related protein 3 (Dkk3), receptor for advanced glycation end products (RAGE) and angiopoietin-2 levels in patients with RSA. Sensitivity and specificity analyses were calculated by a receiver operating characteristics curve, and were revealed to be 0.881, 0.823, 0.79 and 0.814, with diagnostic cut-off points of 95.44 ng/ml for IFGBP-rp1, 32.84 ng/ml for Dkk3, 147.27 ng/ml for RAGE and 441.40 ng/ml for angiopoietin-2. The present study indicated that these four proteins were downregulated in RSA samples and may be useful as biomarkers for the prediction and diagnosis of RSA. Subsequent studies in larger-scale cohorts are required to further validate the diagnostic value of these markers. D.A. Spandidos 2017-09 2017-06-30 /pmc/articles/PMC5547932/ /pubmed/28677727 http://dx.doi.org/10.3892/mmr.2017.6890 Text en Copyright: © Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wu, Ying
He, Junqin
Guo, Chunyu
Zhang, Ying
Yang, Wei
Xin, Mingwei
Liang, Xinyun
Yin, Xiaodan
Wang, Jingshang
Liu, Yanfeng
Serum biomarker analysis in patients with recurrent spontaneous abortion
title Serum biomarker analysis in patients with recurrent spontaneous abortion
title_full Serum biomarker analysis in patients with recurrent spontaneous abortion
title_fullStr Serum biomarker analysis in patients with recurrent spontaneous abortion
title_full_unstemmed Serum biomarker analysis in patients with recurrent spontaneous abortion
title_short Serum biomarker analysis in patients with recurrent spontaneous abortion
title_sort serum biomarker analysis in patients with recurrent spontaneous abortion
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547932/
https://www.ncbi.nlm.nih.gov/pubmed/28677727
http://dx.doi.org/10.3892/mmr.2017.6890
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