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Altered axon initial segment in hippocampal newborn neurons, associated with recurrence of temporal lobe epilepsy in rats

Hippocampal neurogenesis in temporal lobe epilepsy (TLE) may result in alteration of the excitability of neurons, which contributes to spontaneous recurrent seizures. Axon initial segment (AIS) structural and functional plasticity is important in the control of neuronal excitability. It remains to b...

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Autores principales: Liu, Tian-Tian, Feng, Li, Liu, Heng-Fang, Shu, Yi, Xiao, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547972/
https://www.ncbi.nlm.nih.gov/pubmed/28713955
http://dx.doi.org/10.3892/mmr.2017.7017
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author Liu, Tian-Tian
Feng, Li
Liu, Heng-Fang
Shu, Yi
Xiao, Bo
author_facet Liu, Tian-Tian
Feng, Li
Liu, Heng-Fang
Shu, Yi
Xiao, Bo
author_sort Liu, Tian-Tian
collection PubMed
description Hippocampal neurogenesis in temporal lobe epilepsy (TLE) may result in alteration of the excitability of neurons, which contributes to spontaneous recurrent seizures. Axon initial segment (AIS) structural and functional plasticity is important in the control of neuronal excitability. It remains to be elucidated whether the plasticity of AIS occurs in hippocampal newly-generated neurons that are involved in recurrent seizures following pilocarpine-induced status epilepticus (SE). The present study first established a pilocarpine-induced TLE rat model to assess the features of newborn neurons and AIS plasticity alterations using double immunofluorescence staining of Ankyrin G and doublecortin (DCX). AIS plasticity alterations include length and distance from soma in the hippocampal newly-generated neurons post-SE. The results of the present study demonstrated that pilocarpine-induced epileptic rats exhibited aberrant hippocampal neurogenesis and longer DCX-labeled cell dendrites in the dentate gyrus. Pilocarpine-induced epileptic rats demonstrated shortened lengths of AIS and an increased distance from the soma in hippocampal newborn neurons. Mibefradil, a T/L-type calcium blocker, reversed the alterations in length and position of AIS in hippocampal newborn neurons post-SE, accompanied by decreased long-term seizure activity without increased aberrant neurogenesis. These findings indicate that the plasticity of AIS in hippocampal neurogenesis may have profound consequences in epilepsy, at least in animals.
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spelling pubmed-55479722017-10-24 Altered axon initial segment in hippocampal newborn neurons, associated with recurrence of temporal lobe epilepsy in rats Liu, Tian-Tian Feng, Li Liu, Heng-Fang Shu, Yi Xiao, Bo Mol Med Rep Articles Hippocampal neurogenesis in temporal lobe epilepsy (TLE) may result in alteration of the excitability of neurons, which contributes to spontaneous recurrent seizures. Axon initial segment (AIS) structural and functional plasticity is important in the control of neuronal excitability. It remains to be elucidated whether the plasticity of AIS occurs in hippocampal newly-generated neurons that are involved in recurrent seizures following pilocarpine-induced status epilepticus (SE). The present study first established a pilocarpine-induced TLE rat model to assess the features of newborn neurons and AIS plasticity alterations using double immunofluorescence staining of Ankyrin G and doublecortin (DCX). AIS plasticity alterations include length and distance from soma in the hippocampal newly-generated neurons post-SE. The results of the present study demonstrated that pilocarpine-induced epileptic rats exhibited aberrant hippocampal neurogenesis and longer DCX-labeled cell dendrites in the dentate gyrus. Pilocarpine-induced epileptic rats demonstrated shortened lengths of AIS and an increased distance from the soma in hippocampal newborn neurons. Mibefradil, a T/L-type calcium blocker, reversed the alterations in length and position of AIS in hippocampal newborn neurons post-SE, accompanied by decreased long-term seizure activity without increased aberrant neurogenesis. These findings indicate that the plasticity of AIS in hippocampal neurogenesis may have profound consequences in epilepsy, at least in animals. D.A. Spandidos 2017-09 2017-07-15 /pmc/articles/PMC5547972/ /pubmed/28713955 http://dx.doi.org/10.3892/mmr.2017.7017 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Tian-Tian
Feng, Li
Liu, Heng-Fang
Shu, Yi
Xiao, Bo
Altered axon initial segment in hippocampal newborn neurons, associated with recurrence of temporal lobe epilepsy in rats
title Altered axon initial segment in hippocampal newborn neurons, associated with recurrence of temporal lobe epilepsy in rats
title_full Altered axon initial segment in hippocampal newborn neurons, associated with recurrence of temporal lobe epilepsy in rats
title_fullStr Altered axon initial segment in hippocampal newborn neurons, associated with recurrence of temporal lobe epilepsy in rats
title_full_unstemmed Altered axon initial segment in hippocampal newborn neurons, associated with recurrence of temporal lobe epilepsy in rats
title_short Altered axon initial segment in hippocampal newborn neurons, associated with recurrence of temporal lobe epilepsy in rats
title_sort altered axon initial segment in hippocampal newborn neurons, associated with recurrence of temporal lobe epilepsy in rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547972/
https://www.ncbi.nlm.nih.gov/pubmed/28713955
http://dx.doi.org/10.3892/mmr.2017.7017
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