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miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2
The present study aimed to investigate the expression status of miRNA-199a-3p in patients with diabetic neuropathy (DN) and the mechanism by which this miRNA is involved in the genesis of DN. The expression of miRNA-199a-3p in plasma of peripheral blood was compared between patients with diabetes an...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547973/ https://www.ncbi.nlm.nih.gov/pubmed/28677735 http://dx.doi.org/10.3892/mmr.2017.6874 |
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author | Li, Ying-Bo Wu, Qun Liu, Jie Fan, Yong-Zhi Yu, Kai-Feng Cai, Yi |
author_facet | Li, Ying-Bo Wu, Qun Liu, Jie Fan, Yong-Zhi Yu, Kai-Feng Cai, Yi |
author_sort | Li, Ying-Bo |
collection | PubMed |
description | The present study aimed to investigate the expression status of miRNA-199a-3p in patients with diabetic neuropathy (DN) and the mechanism by which this miRNA is involved in the genesis of DN. The expression of miRNA-199a-3p in plasma of peripheral blood was compared between patients with diabetes and a family history of diabetes and control volunteers by reverse transcription-quantitative polymerase chain reaction (RT-qPCR); in 60 diabetes patients, 45 (75%) demosntrated upregulated miR-199a-3p expression compared with control volunteer plasma. RT-qPCR was also used to detect miRNA-199a-3p expression in paired lower limb skin tissues from 30 patients with DN and 20 control volunteers; miR-199a-3p expression in patients with DN was significantly higher than in the control group. Next miR-199a-3p expression levels were evaluated with respect to the clinic-pathological parameters of diabetes; increased expression of miR-199a-3p was significantly associated with increased disease duration (P=0.041), glycated hemoglobin (HbA1C) levels (P=0.033), and fibrinogen levels (P=0.003). Finally, the effects on downstream mRNA expression levels were investigated as a result of manipulating miR-199a-3p levels. miR-199a-3p overexpression inhibited the expression of the extracellular serine protease inhibitor E2 (SerpinE2). Therefore, it may be hypothesized that miR-199a-3p can induce DN via promoting coagulation in skin peripheral circulation, through the downregulation of SerpinE2. The present findings suggested that miR-199a-3p may have potential as a novel therapeutic target for the treatment of patients with DN. |
format | Online Article Text |
id | pubmed-5547973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-55479732017-10-24 miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2 Li, Ying-Bo Wu, Qun Liu, Jie Fan, Yong-Zhi Yu, Kai-Feng Cai, Yi Mol Med Rep Articles The present study aimed to investigate the expression status of miRNA-199a-3p in patients with diabetic neuropathy (DN) and the mechanism by which this miRNA is involved in the genesis of DN. The expression of miRNA-199a-3p in plasma of peripheral blood was compared between patients with diabetes and a family history of diabetes and control volunteers by reverse transcription-quantitative polymerase chain reaction (RT-qPCR); in 60 diabetes patients, 45 (75%) demosntrated upregulated miR-199a-3p expression compared with control volunteer plasma. RT-qPCR was also used to detect miRNA-199a-3p expression in paired lower limb skin tissues from 30 patients with DN and 20 control volunteers; miR-199a-3p expression in patients with DN was significantly higher than in the control group. Next miR-199a-3p expression levels were evaluated with respect to the clinic-pathological parameters of diabetes; increased expression of miR-199a-3p was significantly associated with increased disease duration (P=0.041), glycated hemoglobin (HbA1C) levels (P=0.033), and fibrinogen levels (P=0.003). Finally, the effects on downstream mRNA expression levels were investigated as a result of manipulating miR-199a-3p levels. miR-199a-3p overexpression inhibited the expression of the extracellular serine protease inhibitor E2 (SerpinE2). Therefore, it may be hypothesized that miR-199a-3p can induce DN via promoting coagulation in skin peripheral circulation, through the downregulation of SerpinE2. The present findings suggested that miR-199a-3p may have potential as a novel therapeutic target for the treatment of patients with DN. D.A. Spandidos 2017-09 2017-06-28 /pmc/articles/PMC5547973/ /pubmed/28677735 http://dx.doi.org/10.3892/mmr.2017.6874 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Ying-Bo Wu, Qun Liu, Jie Fan, Yong-Zhi Yu, Kai-Feng Cai, Yi miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2 |
title | miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2 |
title_full | miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2 |
title_fullStr | miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2 |
title_full_unstemmed | miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2 |
title_short | miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2 |
title_sort | mir-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of serpine2 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547973/ https://www.ncbi.nlm.nih.gov/pubmed/28677735 http://dx.doi.org/10.3892/mmr.2017.6874 |
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