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Expression profile of circular RNAs in human gastric cancer tissues

Circular RNAs (circRNAs) represent a newly identified class of non-coding RNA molecules, which interfere with gene transcription by adsorbing microRNAs (miRNAs). CircRNAs serve important roles in disease development and have the potential to serve as a novel class of biomarkers for clinical diagnosi...

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Autores principales: Huang, You-Sheng, Jie, Na, Zou, Ke-Jian, Weng, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548015/
https://www.ncbi.nlm.nih.gov/pubmed/28737829
http://dx.doi.org/10.3892/mmr.2017.6916
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author Huang, You-Sheng
Jie, Na
Zou, Ke-Jian
Weng, Yang
author_facet Huang, You-Sheng
Jie, Na
Zou, Ke-Jian
Weng, Yang
author_sort Huang, You-Sheng
collection PubMed
description Circular RNAs (circRNAs) represent a newly identified class of non-coding RNA molecules, which interfere with gene transcription by adsorbing microRNAs (miRNAs). CircRNAs serve important roles in disease development and have the potential to serve as a novel class of biomarkers for clinical diagnosis. However, the role of circRNAs in the occurrence and development of gastric cancer (GC) remains unclear. In the present study, the expression profiles of circRNAs were compared between GC and adjacent normal tissues using a circRNA microarray, following which quantitative polymerase chain reaction (qPCR) was used to confirm the results of the circRNA microarray. Compared with the adjacent, normal mucosal tissues, 16 circRNAs were upregulated and 84 circRNAs were downregulated in GC. A total of 10 circRNAs were selected for validation in three pairs of GC and adjacent noncancerous tissues. The qPCR results were consistent with the findings of the microarray-based expression analysis. Of the circRNAs studied, only circRNA-0026 (hsa_circ_0000026) exhibited significantly different expression in GC (2.8-fold, P=0.001). Furthermore, online Database for Annotation, Visualization and Integrated Discovery annotation was used to predict circRNA-targeted miRNA-gene interactions. The analysis revealed that circRNA-0026 may regulate RNA transcription, RNA metabolism, gene expression, gene silencing and other biological functions in GC. In conclusion, differential expression of circRNAs may be associated with GC tumorigenesis, and circRNA-0026 is a promising biomarker for GC diagnosis and targeted therapy.
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spelling pubmed-55480152017-10-24 Expression profile of circular RNAs in human gastric cancer tissues Huang, You-Sheng Jie, Na Zou, Ke-Jian Weng, Yang Mol Med Rep Articles Circular RNAs (circRNAs) represent a newly identified class of non-coding RNA molecules, which interfere with gene transcription by adsorbing microRNAs (miRNAs). CircRNAs serve important roles in disease development and have the potential to serve as a novel class of biomarkers for clinical diagnosis. However, the role of circRNAs in the occurrence and development of gastric cancer (GC) remains unclear. In the present study, the expression profiles of circRNAs were compared between GC and adjacent normal tissues using a circRNA microarray, following which quantitative polymerase chain reaction (qPCR) was used to confirm the results of the circRNA microarray. Compared with the adjacent, normal mucosal tissues, 16 circRNAs were upregulated and 84 circRNAs were downregulated in GC. A total of 10 circRNAs were selected for validation in three pairs of GC and adjacent noncancerous tissues. The qPCR results were consistent with the findings of the microarray-based expression analysis. Of the circRNAs studied, only circRNA-0026 (hsa_circ_0000026) exhibited significantly different expression in GC (2.8-fold, P=0.001). Furthermore, online Database for Annotation, Visualization and Integrated Discovery annotation was used to predict circRNA-targeted miRNA-gene interactions. The analysis revealed that circRNA-0026 may regulate RNA transcription, RNA metabolism, gene expression, gene silencing and other biological functions in GC. In conclusion, differential expression of circRNAs may be associated with GC tumorigenesis, and circRNA-0026 is a promising biomarker for GC diagnosis and targeted therapy. D.A. Spandidos 2017-09 2017-07-04 /pmc/articles/PMC5548015/ /pubmed/28737829 http://dx.doi.org/10.3892/mmr.2017.6916 Text en Copyright: © Huang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Huang, You-Sheng
Jie, Na
Zou, Ke-Jian
Weng, Yang
Expression profile of circular RNAs in human gastric cancer tissues
title Expression profile of circular RNAs in human gastric cancer tissues
title_full Expression profile of circular RNAs in human gastric cancer tissues
title_fullStr Expression profile of circular RNAs in human gastric cancer tissues
title_full_unstemmed Expression profile of circular RNAs in human gastric cancer tissues
title_short Expression profile of circular RNAs in human gastric cancer tissues
title_sort expression profile of circular rnas in human gastric cancer tissues
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548015/
https://www.ncbi.nlm.nih.gov/pubmed/28737829
http://dx.doi.org/10.3892/mmr.2017.6916
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