Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy

Photodamage to Photosystem II (PSII) has been attributed either to excessive excitation of photosynthetic pigments or by direct of light absorption by Mn(4)CaO(5) cluster. Here we investigated the time course of PSII photodamage and release of Mn in PSII-enriched membranes under high light illuminat...

Descripción completa

Detalles Bibliográficos
Autores principales: Zavafer, Alonso, Koinuma, Wataru, Chow, Wah Soon, Cheah, Mun Hon, Mino, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548768/
https://www.ncbi.nlm.nih.gov/pubmed/28790352
http://dx.doi.org/10.1038/s41598-017-07671-1
_version_ 1783255872720338944
author Zavafer, Alonso
Koinuma, Wataru
Chow, Wah Soon
Cheah, Mun Hon
Mino, Hiroyuki
author_facet Zavafer, Alonso
Koinuma, Wataru
Chow, Wah Soon
Cheah, Mun Hon
Mino, Hiroyuki
author_sort Zavafer, Alonso
collection PubMed
description Photodamage to Photosystem II (PSII) has been attributed either to excessive excitation of photosynthetic pigments or by direct of light absorption by Mn(4)CaO(5) cluster. Here we investigated the time course of PSII photodamage and release of Mn in PSII-enriched membranes under high light illumination at 460 nm and 660 nm. We found that the loss of PSII activity, assayed by chlorophyll fluorescence, is faster than release of Mn from the Mn(4)CaO(5) cluster, assayed by EPR. Loss of PSII activity and Mn release was slower during illumination in the presence of exogenous electron acceptors. Recovery of PSII activity was observed, after 30 min of addition of electron donor post illumination. The same behavior was observed under 460 and 660 nm illumination, suggesting stronger correlation between excessive excitation and photodamage compared to direct light absorption by the cluster. A unified model of PSII photodamage that takes into account present and previous literature reports is presented.
format Online
Article
Text
id pubmed-5548768
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-55487682017-08-09 Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy Zavafer, Alonso Koinuma, Wataru Chow, Wah Soon Cheah, Mun Hon Mino, Hiroyuki Sci Rep Article Photodamage to Photosystem II (PSII) has been attributed either to excessive excitation of photosynthetic pigments or by direct of light absorption by Mn(4)CaO(5) cluster. Here we investigated the time course of PSII photodamage and release of Mn in PSII-enriched membranes under high light illumination at 460 nm and 660 nm. We found that the loss of PSII activity, assayed by chlorophyll fluorescence, is faster than release of Mn from the Mn(4)CaO(5) cluster, assayed by EPR. Loss of PSII activity and Mn release was slower during illumination in the presence of exogenous electron acceptors. Recovery of PSII activity was observed, after 30 min of addition of electron donor post illumination. The same behavior was observed under 460 and 660 nm illumination, suggesting stronger correlation between excessive excitation and photodamage compared to direct light absorption by the cluster. A unified model of PSII photodamage that takes into account present and previous literature reports is presented. Nature Publishing Group UK 2017-08-08 /pmc/articles/PMC5548768/ /pubmed/28790352 http://dx.doi.org/10.1038/s41598-017-07671-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zavafer, Alonso
Koinuma, Wataru
Chow, Wah Soon
Cheah, Mun Hon
Mino, Hiroyuki
Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
title Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
title_full Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
title_fullStr Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
title_full_unstemmed Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
title_short Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
title_sort mechanism of photodamage of the oxygen evolving mn cluster of photosystem ii by excessive light energy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548768/
https://www.ncbi.nlm.nih.gov/pubmed/28790352
http://dx.doi.org/10.1038/s41598-017-07671-1
work_keys_str_mv AT zavaferalonso mechanismofphotodamageoftheoxygenevolvingmnclusterofphotosystemiibyexcessivelightenergy
AT koinumawataru mechanismofphotodamageoftheoxygenevolvingmnclusterofphotosystemiibyexcessivelightenergy
AT chowwahsoon mechanismofphotodamageoftheoxygenevolvingmnclusterofphotosystemiibyexcessivelightenergy
AT cheahmunhon mechanismofphotodamageoftheoxygenevolvingmnclusterofphotosystemiibyexcessivelightenergy
AT minohiroyuki mechanismofphotodamageoftheoxygenevolvingmnclusterofphotosystemiibyexcessivelightenergy

Ejemplares similares