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CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus

CRISPR/Cas9 is a valuable tool for both basic and applied research that has been widely applied to different plant species. Nonetheless, a systematical assessment of the efficiency of this method is not available for the allotetraploid Brassica napus—an important oilseed crop. In this study, we exam...

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Detalles Bibliográficos
Autores principales: Yang, Hong, Wu, Jia-Jing, Tang, Ting, Liu, Ke-De, Dai, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548805/
https://www.ncbi.nlm.nih.gov/pubmed/28790350
http://dx.doi.org/10.1038/s41598-017-07871-9