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SFPQ, a multifunctional nuclear protein, regulates the transcription of PDE3A
Phosphodiesterase 3A (PDE3A), a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (PDE) family, plays important roles in oocyte maturation and vascular smooth muscle cell proliferation. However, the molecular mechanisms that regulate PDE3A gene expression remain largely unknown. In th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548871/ https://www.ncbi.nlm.nih.gov/pubmed/28743736 http://dx.doi.org/10.1042/BSR20170975 |
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author | Rhee, Dong Keun Hockman, Steven C. Choi, Sunkyung Kim, Yong-Eun Park, Chungoo Manganiello, Vincent C. Kim, Kee K. |
author_facet | Rhee, Dong Keun Hockman, Steven C. Choi, Sunkyung Kim, Yong-Eun Park, Chungoo Manganiello, Vincent C. Kim, Kee K. |
author_sort | Rhee, Dong Keun |
collection | PubMed |
description | Phosphodiesterase 3A (PDE3A), a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (PDE) family, plays important roles in oocyte maturation and vascular smooth muscle cell proliferation. However, the molecular mechanisms that regulate PDE3A gene expression remain largely unknown. In the present study, we investigated the transcriptional regulation of PDE3A, and found that the splicing factor proline- and glutamine-rich (SFPQ) protein modulated PDE3A mRNA levels. Multiple transcription start sites (TSS1, 2, and 3) were identified within the first exon of PDE3A using 5′-rapid amplification of cDNA ends (RACE). Variable expression levels of three PDE3A variants were also observed in human tissues and HeLa cells. Several putative SFPQ-binding sites were identified upstream of the regulatory region of PDE3A-TSSs using ChIP sequencing (ChIP-seq). Serum-induced PDE3A expression was affected by increasing the amount of SFPQ binding to the upstream regulatory region of PDE3A. In addition, transcription of PDE3A was lower in human cervical adenocarcinoma cells compared with normal cervical tissue. Furthermore, overexpression of PDE3A induced sensitivity to anticancer therapeutic agent, 6-(4-(diethylamino)-3-nitrophenyl)-5-methyl-4,5-dihydropyridazin-3(2H)-one (DNMDP), in HeLa cells. Taken together, these results suggest that SFPQ functions as a transcriptional activator of PDE3A, which is involved in the regulation of DNMDP sensitivity, offering a novel molecular target for the development of anticancer therapies. |
format | Online Article Text |
id | pubmed-5548871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55488712017-08-24 SFPQ, a multifunctional nuclear protein, regulates the transcription of PDE3A Rhee, Dong Keun Hockman, Steven C. Choi, Sunkyung Kim, Yong-Eun Park, Chungoo Manganiello, Vincent C. Kim, Kee K. Biosci Rep Research Articles Phosphodiesterase 3A (PDE3A), a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (PDE) family, plays important roles in oocyte maturation and vascular smooth muscle cell proliferation. However, the molecular mechanisms that regulate PDE3A gene expression remain largely unknown. In the present study, we investigated the transcriptional regulation of PDE3A, and found that the splicing factor proline- and glutamine-rich (SFPQ) protein modulated PDE3A mRNA levels. Multiple transcription start sites (TSS1, 2, and 3) were identified within the first exon of PDE3A using 5′-rapid amplification of cDNA ends (RACE). Variable expression levels of three PDE3A variants were also observed in human tissues and HeLa cells. Several putative SFPQ-binding sites were identified upstream of the regulatory region of PDE3A-TSSs using ChIP sequencing (ChIP-seq). Serum-induced PDE3A expression was affected by increasing the amount of SFPQ binding to the upstream regulatory region of PDE3A. In addition, transcription of PDE3A was lower in human cervical adenocarcinoma cells compared with normal cervical tissue. Furthermore, overexpression of PDE3A induced sensitivity to anticancer therapeutic agent, 6-(4-(diethylamino)-3-nitrophenyl)-5-methyl-4,5-dihydropyridazin-3(2H)-one (DNMDP), in HeLa cells. Taken together, these results suggest that SFPQ functions as a transcriptional activator of PDE3A, which is involved in the regulation of DNMDP sensitivity, offering a novel molecular target for the development of anticancer therapies. Portland Press Ltd. 2017-08-09 /pmc/articles/PMC5548871/ /pubmed/28743736 http://dx.doi.org/10.1042/BSR20170975 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Rhee, Dong Keun Hockman, Steven C. Choi, Sunkyung Kim, Yong-Eun Park, Chungoo Manganiello, Vincent C. Kim, Kee K. SFPQ, a multifunctional nuclear protein, regulates the transcription of PDE3A |
title | SFPQ, a multifunctional nuclear protein, regulates the transcription of PDE3A |
title_full | SFPQ, a multifunctional nuclear protein, regulates the transcription of PDE3A |
title_fullStr | SFPQ, a multifunctional nuclear protein, regulates the transcription of PDE3A |
title_full_unstemmed | SFPQ, a multifunctional nuclear protein, regulates the transcription of PDE3A |
title_short | SFPQ, a multifunctional nuclear protein, regulates the transcription of PDE3A |
title_sort | sfpq, a multifunctional nuclear protein, regulates the transcription of pde3a |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548871/ https://www.ncbi.nlm.nih.gov/pubmed/28743736 http://dx.doi.org/10.1042/BSR20170975 |
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