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Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma

Esophageal cancer ranks as the sixth leading cause of cancer-related deaths worldwide. Cancer stemness is mainly considered to be the key factor for cancer recurrence particularly in esophageal cancer. It is important to identify cancer stem cell markers as targets in future therapies. The present s...

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Autores principales: Qin, Guohui, Lian, Jingyao, Yue, Dongli, Chen, Xinfeng, Nan, Shufeng, Qi, Yu, Li, Bing, Cui, Guanghui, Li, Xiangnan, Zhao, Song, Zhang, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549024/
https://www.ncbi.nlm.nih.gov/pubmed/28713964
http://dx.doi.org/10.3892/or.2017.5809
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author Qin, Guohui
Lian, Jingyao
Yue, Dongli
Chen, Xinfeng
Nan, Shufeng
Qi, Yu
Li, Bing
Cui, Guanghui
Li, Xiangnan
Zhao, Song
Zhang, Yi
author_facet Qin, Guohui
Lian, Jingyao
Yue, Dongli
Chen, Xinfeng
Nan, Shufeng
Qi, Yu
Li, Bing
Cui, Guanghui
Li, Xiangnan
Zhao, Song
Zhang, Yi
author_sort Qin, Guohui
collection PubMed
description Esophageal cancer ranks as the sixth leading cause of cancer-related deaths worldwide. Cancer stemness is mainly considered to be the key factor for cancer recurrence particularly in esophageal cancer. It is important to identify cancer stem cell markers as targets in future therapies. The present study aimed to investigate the expression of putative cancer stem cell-related marker musashi1 (Msi1) and assess the correlation with clinicopathologcal status of esophageal squamous cell carcinoma (ESCC) cases. We then clarified the role of Msi1 in esophageal cancer cells during proliferation, apoptosis, sphere formation and migration. Finally, we investigated the relationship of Msi1 with the prognosis of ESCC patients. ESCC tissue samples from 93 patients and 20 paired histologically normal tissues were procured for immunohistochemical analysis. We analyzed the characteristics of Msi1, using sphere formation and anchorage independent growth. Moreover, using flow cytometry and Cell Counting Kit-8 (CCK-8) assay, we investigated the role of Msi1 in cancer cell proliferation and apoptosis. Furthermore, we clarified the role of Msi1 in the process of sphere formation and migration of ESCC cells through knockdown of Msi1 expression by siRNA in ESCC cell lines. The results revealed that there was a higher expression of Msi1 in ESCC specimens compared with normal tissues. In addition, Msi1 expression was significantly associated with clinical stage and lymph node metastasis. Most importantly, the increased immunocytochemical staining of Msi1 in spheroid cells revealed the stemness characteristics of Msi1 in ESCC. In addition, we found that silencing of Msi1 decreased cell proliferation, migration and induced apoptosis in TE-7 and KYSE70 cells. Furthermore, downregulation of Msi1 attenuated the sphere formation ability of ESCC cells. Patients with higher expression of Msi1 had a shorter survival. In conclusion, Msi1 acts as a stemness-associated gene in esophageal cancer cell lines and could serve as a prognostic marker in patients with ESCC.
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spelling pubmed-55490242018-09-01 Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma Qin, Guohui Lian, Jingyao Yue, Dongli Chen, Xinfeng Nan, Shufeng Qi, Yu Li, Bing Cui, Guanghui Li, Xiangnan Zhao, Song Zhang, Yi Oncol Rep Articles Esophageal cancer ranks as the sixth leading cause of cancer-related deaths worldwide. Cancer stemness is mainly considered to be the key factor for cancer recurrence particularly in esophageal cancer. It is important to identify cancer stem cell markers as targets in future therapies. The present study aimed to investigate the expression of putative cancer stem cell-related marker musashi1 (Msi1) and assess the correlation with clinicopathologcal status of esophageal squamous cell carcinoma (ESCC) cases. We then clarified the role of Msi1 in esophageal cancer cells during proliferation, apoptosis, sphere formation and migration. Finally, we investigated the relationship of Msi1 with the prognosis of ESCC patients. ESCC tissue samples from 93 patients and 20 paired histologically normal tissues were procured for immunohistochemical analysis. We analyzed the characteristics of Msi1, using sphere formation and anchorage independent growth. Moreover, using flow cytometry and Cell Counting Kit-8 (CCK-8) assay, we investigated the role of Msi1 in cancer cell proliferation and apoptosis. Furthermore, we clarified the role of Msi1 in the process of sphere formation and migration of ESCC cells through knockdown of Msi1 expression by siRNA in ESCC cell lines. The results revealed that there was a higher expression of Msi1 in ESCC specimens compared with normal tissues. In addition, Msi1 expression was significantly associated with clinical stage and lymph node metastasis. Most importantly, the increased immunocytochemical staining of Msi1 in spheroid cells revealed the stemness characteristics of Msi1 in ESCC. In addition, we found that silencing of Msi1 decreased cell proliferation, migration and induced apoptosis in TE-7 and KYSE70 cells. Furthermore, downregulation of Msi1 attenuated the sphere formation ability of ESCC cells. Patients with higher expression of Msi1 had a shorter survival. In conclusion, Msi1 acts as a stemness-associated gene in esophageal cancer cell lines and could serve as a prognostic marker in patients with ESCC. D.A. Spandidos 2017-09 2017-07-12 /pmc/articles/PMC5549024/ /pubmed/28713964 http://dx.doi.org/10.3892/or.2017.5809 Text en Copyright © 2017, Spandidos Publications
spellingShingle Articles
Qin, Guohui
Lian, Jingyao
Yue, Dongli
Chen, Xinfeng
Nan, Shufeng
Qi, Yu
Li, Bing
Cui, Guanghui
Li, Xiangnan
Zhao, Song
Zhang, Yi
Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma
title Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma
title_full Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma
title_fullStr Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma
title_full_unstemmed Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma
title_short Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma
title_sort musashi1, a potential prognostic marker in esophageal squamous cell carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549024/
https://www.ncbi.nlm.nih.gov/pubmed/28713964
http://dx.doi.org/10.3892/or.2017.5809
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