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Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma
Long non-coding RNAs (lncRNAs) are continuously transcribed and are involved in various cellular activities. However, their contributions to the occurrence and development of germinal center B-cell (GCB)-like diffuse large B-cell lymphoma (DLBCL) remain largely unknown. We applied microarray technol...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549037/ https://www.ncbi.nlm.nih.gov/pubmed/28713985 http://dx.doi.org/10.3892/or.2017.5821 |
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author | Gao, Hong-Yu Wu, Bin Yan, Wei Gong, Zi-Mu Sun, Qi Wang, Hui-Han Yang, Wei |
author_facet | Gao, Hong-Yu Wu, Bin Yan, Wei Gong, Zi-Mu Sun, Qi Wang, Hui-Han Yang, Wei |
author_sort | Gao, Hong-Yu |
collection | PubMed |
description | Long non-coding RNAs (lncRNAs) are continuously transcribed and are involved in various cellular activities. However, their contributions to the occurrence and development of germinal center B-cell (GCB)-like diffuse large B-cell lymphoma (DLBCL) remain largely unknown. We applied microarray technology to profile the expression of lncRNAs in two different GCB-DLBCL cell lines (OCI-ly1 and OCI-ly19) and normal B lymphocytes. We demonstrated that 21,539 lncRNAs were expressed in all of the samples analyzed. This included 1,648 lncRNAs that showed a ≥2-fold upregulation and 2,671 lncRNAs that displayed a ≥2-fold downregulation in tumor cell lines (P<0.05). The expression levels of 8 lncRNAs were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Bioinformatic analyses (Gene Ontology, pathway and network analysis) were performed to predict how the differentially expressed lncRNAs may function in GCB-DLBCL. Results from the pathway analysis suggested that totals of 64 and 62 biological pathways corresponded to upregulated and downregulated transcripts, respectively (P<0.05). Additionally, we constructed a lncRNA-mRNA network for the purpose of identifying specific coding genes which were co-expressed with 5 selected lncRNAs. Conclusively, our results may contribute to a better understanding of GCB-DLBCL pathogenesis. |
format | Online Article Text |
id | pubmed-5549037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-55490372017-11-02 Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma Gao, Hong-Yu Wu, Bin Yan, Wei Gong, Zi-Mu Sun, Qi Wang, Hui-Han Yang, Wei Oncol Rep Articles Long non-coding RNAs (lncRNAs) are continuously transcribed and are involved in various cellular activities. However, their contributions to the occurrence and development of germinal center B-cell (GCB)-like diffuse large B-cell lymphoma (DLBCL) remain largely unknown. We applied microarray technology to profile the expression of lncRNAs in two different GCB-DLBCL cell lines (OCI-ly1 and OCI-ly19) and normal B lymphocytes. We demonstrated that 21,539 lncRNAs were expressed in all of the samples analyzed. This included 1,648 lncRNAs that showed a ≥2-fold upregulation and 2,671 lncRNAs that displayed a ≥2-fold downregulation in tumor cell lines (P<0.05). The expression levels of 8 lncRNAs were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Bioinformatic analyses (Gene Ontology, pathway and network analysis) were performed to predict how the differentially expressed lncRNAs may function in GCB-DLBCL. Results from the pathway analysis suggested that totals of 64 and 62 biological pathways corresponded to upregulated and downregulated transcripts, respectively (P<0.05). Additionally, we constructed a lncRNA-mRNA network for the purpose of identifying specific coding genes which were co-expressed with 5 selected lncRNAs. Conclusively, our results may contribute to a better understanding of GCB-DLBCL pathogenesis. D.A. Spandidos 2017-09 2017-07-14 /pmc/articles/PMC5549037/ /pubmed/28713985 http://dx.doi.org/10.3892/or.2017.5821 Text en Copyright: © Gao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Gao, Hong-Yu Wu, Bin Yan, Wei Gong, Zi-Mu Sun, Qi Wang, Hui-Han Yang, Wei Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma |
title | Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma |
title_full | Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma |
title_fullStr | Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma |
title_full_unstemmed | Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma |
title_short | Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma |
title_sort | microarray expression profiles of long non-coding rnas in germinal center-like diffuse large b-cell lymphoma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549037/ https://www.ncbi.nlm.nih.gov/pubmed/28713985 http://dx.doi.org/10.3892/or.2017.5821 |
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