Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1

CFE-1 is a unique plasmid-encoded AmpC β-lactamase with the regulator gene ampR. It imparts high resistance to most cephalosporins with constitutive high-level β-lactamase activity. Here, the β-lactamase activities and expression levels of ampC with or without ampR were investigated. Results suggest...

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Detalles Bibliográficos
Autores principales: Nakano, Ryuichi, Nakano, Akiyo, Yano, Hisakazu, Okamoto, Ryoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2017
Materias:
Opinion/Hypothesis
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549177/
https://www.ncbi.nlm.nih.gov/pubmed/28808689
http://dx.doi.org/10.1128/mSphere.00192-17
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author Nakano, Ryuichi
Nakano, Akiyo
Yano, Hisakazu
Okamoto, Ryoichi
author_facet Nakano, Ryuichi
Nakano, Akiyo
Yano, Hisakazu
Okamoto, Ryoichi
author_sort Nakano, Ryuichi
collection PubMed
description CFE-1 is a unique plasmid-encoded AmpC β-lactamase with the regulator gene ampR. It imparts high resistance to most cephalosporins with constitutive high-level β-lactamase activity. Here, the β-lactamase activities and expression levels of ampC with or without ampR were investigated. Results suggested that the resistance of CFE-1 to cephalosporins is caused by a substitution in AmpR, in which the Asp at position 135 is modified to Ala to allow the constitutive high-level expression (derepression) of ampC.
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spelling pubmed-55491772017-08-14 Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1 Nakano, Ryuichi Nakano, Akiyo Yano, Hisakazu Okamoto, Ryoichi mSphere Opinion/Hypothesis CFE-1 is a unique plasmid-encoded AmpC β-lactamase with the regulator gene ampR. It imparts high resistance to most cephalosporins with constitutive high-level β-lactamase activity. Here, the β-lactamase activities and expression levels of ampC with or without ampR were investigated. Results suggested that the resistance of CFE-1 to cephalosporins is caused by a substitution in AmpR, in which the Asp at position 135 is modified to Ala to allow the constitutive high-level expression (derepression) of ampC. American Society for Microbiology 2017-08-09 /pmc/articles/PMC5549177/ /pubmed/28808689 http://dx.doi.org/10.1128/mSphere.00192-17 Text en Copyright © 2017 Nakano et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Opinion/Hypothesis
Nakano, Ryuichi
Nakano, Akiyo
Yano, Hisakazu
Okamoto, Ryoichi
Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
title Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
title_full Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
title_fullStr Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
title_full_unstemmed Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
title_short Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
title_sort role of ampr in the high expression of the plasmid-encoded ampc β-lactamase cfe-1
topic Opinion/Hypothesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549177/
https://www.ncbi.nlm.nih.gov/pubmed/28808689
http://dx.doi.org/10.1128/mSphere.00192-17
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