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Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting

BACKGROUND: Malaria is a red blood cell (RBC) infection caused by Plasmodium parasites. To determine RBC infection rate, which is essential for malaria study and diagnosis, microscopic evaluation of Giemsa-stained thin blood smears on glass slides (‘Giemsa microscopy’) has been performed as the acce...

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Autores principales: Hashimoto, Muneaki, Yatsushiro, Shouki, Yamamura, Shohei, Tanaka, Masato, Sakamoto, Hirokazu, Ido, Yusuke, Kajimoto, Kazuaki, Bando, Mika, Kido, Jun-ichi, Kataoka, Masatoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549322/
https://www.ncbi.nlm.nih.gov/pubmed/28789644
http://dx.doi.org/10.1186/s12936-017-1975-9
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author Hashimoto, Muneaki
Yatsushiro, Shouki
Yamamura, Shohei
Tanaka, Masato
Sakamoto, Hirokazu
Ido, Yusuke
Kajimoto, Kazuaki
Bando, Mika
Kido, Jun-ichi
Kataoka, Masatoshi
author_facet Hashimoto, Muneaki
Yatsushiro, Shouki
Yamamura, Shohei
Tanaka, Masato
Sakamoto, Hirokazu
Ido, Yusuke
Kajimoto, Kazuaki
Bando, Mika
Kido, Jun-ichi
Kataoka, Masatoshi
author_sort Hashimoto, Muneaki
collection PubMed
description BACKGROUND: Malaria is a red blood cell (RBC) infection caused by Plasmodium parasites. To determine RBC infection rate, which is essential for malaria study and diagnosis, microscopic evaluation of Giemsa-stained thin blood smears on glass slides (‘Giemsa microscopy’) has been performed as the accepted gold standard for over 100 years. However, only a small area of the blood smear provides a monolayer of RBCs suitable for determination of infection rate, which is one of the major reasons for the low parasite detection rate by Giemsa microscopy. In addition, because Giemsa microscopy is exacting and time-consuming, automated counting of infection rates is highly desirable. RESULTS: A method that allows for microscopic examination of Giemsa-stained cells spread in a monolayer on almost the whole surface of hydrophilic-treated cyclic olefin copolymer (COC) plates was established. Because wide-range Giemsa microscopy can be performed on a hydrophilic-treated plate, the method may enable more reliable diagnosis of malaria in patients with low parasitaemia burden. Furthermore, the number of RBCs and parasites stained with a fluorescent nuclear staining dye could be counted automatically with a software tool, without Giemsa staining. As a result, researchers studying malaria may calculate the infection rate easily, rapidly, and accurately even in low parasitaemia. CONCLUSION: Because the running cost of these methods is very low and they do not involve complicated techniques, the use of hydrophilic COC plates may contribute to improved and more accurate diagnosis and research of malaria. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12936-017-1975-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-55493222017-08-11 Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting Hashimoto, Muneaki Yatsushiro, Shouki Yamamura, Shohei Tanaka, Masato Sakamoto, Hirokazu Ido, Yusuke Kajimoto, Kazuaki Bando, Mika Kido, Jun-ichi Kataoka, Masatoshi Malar J Methodology BACKGROUND: Malaria is a red blood cell (RBC) infection caused by Plasmodium parasites. To determine RBC infection rate, which is essential for malaria study and diagnosis, microscopic evaluation of Giemsa-stained thin blood smears on glass slides (‘Giemsa microscopy’) has been performed as the accepted gold standard for over 100 years. However, only a small area of the blood smear provides a monolayer of RBCs suitable for determination of infection rate, which is one of the major reasons for the low parasite detection rate by Giemsa microscopy. In addition, because Giemsa microscopy is exacting and time-consuming, automated counting of infection rates is highly desirable. RESULTS: A method that allows for microscopic examination of Giemsa-stained cells spread in a monolayer on almost the whole surface of hydrophilic-treated cyclic olefin copolymer (COC) plates was established. Because wide-range Giemsa microscopy can be performed on a hydrophilic-treated plate, the method may enable more reliable diagnosis of malaria in patients with low parasitaemia burden. Furthermore, the number of RBCs and parasites stained with a fluorescent nuclear staining dye could be counted automatically with a software tool, without Giemsa staining. As a result, researchers studying malaria may calculate the infection rate easily, rapidly, and accurately even in low parasitaemia. CONCLUSION: Because the running cost of these methods is very low and they do not involve complicated techniques, the use of hydrophilic COC plates may contribute to improved and more accurate diagnosis and research of malaria. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12936-017-1975-9) contains supplementary material, which is available to authorized users. BioMed Central 2017-08-08 /pmc/articles/PMC5549322/ /pubmed/28789644 http://dx.doi.org/10.1186/s12936-017-1975-9 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology
Hashimoto, Muneaki
Yatsushiro, Shouki
Yamamura, Shohei
Tanaka, Masato
Sakamoto, Hirokazu
Ido, Yusuke
Kajimoto, Kazuaki
Bando, Mika
Kido, Jun-ichi
Kataoka, Masatoshi
Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting
title Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting
title_full Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting
title_fullStr Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting
title_full_unstemmed Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting
title_short Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting
title_sort hydrophilic-treated plastic plates for wide-range analysis of giemsa-stained red blood cells and automated plasmodium infection rate counting
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549322/
https://www.ncbi.nlm.nih.gov/pubmed/28789644
http://dx.doi.org/10.1186/s12936-017-1975-9
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