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Cloning and expression analysis of BmYki gene in silkworm, Bombyx mori

The transcriptional coactivator Yorkie(Yki), is a critical downstream effector of the Hippo(Hpo) signaling pathway that controls organ size through the regulation of cell proliferation and apoptosis. During the past ten years the biological function of Yki has been studied extensively in Drosophila...

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Autores principales: Zeng, Wenhui, Wang, Riyuan, Zhang, Tianyang, Gong, Chunying, Zuo, Weidong, Liu, Rongpeng, Ou, Yao, Xu, Hanfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549978/
https://www.ncbi.nlm.nih.gov/pubmed/28793345
http://dx.doi.org/10.1371/journal.pone.0182690
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author Zeng, Wenhui
Wang, Riyuan
Zhang, Tianyang
Gong, Chunying
Zuo, Weidong
Liu, Rongpeng
Ou, Yao
Xu, Hanfu
author_facet Zeng, Wenhui
Wang, Riyuan
Zhang, Tianyang
Gong, Chunying
Zuo, Weidong
Liu, Rongpeng
Ou, Yao
Xu, Hanfu
author_sort Zeng, Wenhui
collection PubMed
description The transcriptional coactivator Yorkie(Yki), is a critical downstream effector of the Hippo(Hpo) signaling pathway that controls organ size through the regulation of cell proliferation and apoptosis. During the past ten years the biological function of Yki has been studied extensively in Drosophila and a few other insects, however, little is known about it in the silkworm, Bombyx mori, a major research model of lepidopteran insect. Here, we describe the isolation, characterization and expression of the B. mori Yki ortholog, BmYki. The coding sequence of the BmYki was 1314 bp in length, encoding a protein of 437 amino acids containing two conserved WW domains. BmYki transcripts were ubiquitous but not abundant in all detected tissues and developmental stages. Comparatively, it was expressed at pretty high level in silk glands and at the stage of fifth-instar day-3 larvae. Overexpression of BmYki in cultured B. mori embryonic cells significantly promoted transcription of genes associated with cell proliferation and apoptosis, indicating that BmYki functions in the regulation of organ growth-related biological processes. Interestingly, transcription of silk protein-coding genes and transcription factors regulating the synthesis of silk proteins was downregulated remarkably, suggesting that BmYki was involved in the regulation of silk protein synthesis. This study provides new insights into the role of BmYki in Hpo pathway regulation in silkworm.
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spelling pubmed-55499782017-08-15 Cloning and expression analysis of BmYki gene in silkworm, Bombyx mori Zeng, Wenhui Wang, Riyuan Zhang, Tianyang Gong, Chunying Zuo, Weidong Liu, Rongpeng Ou, Yao Xu, Hanfu PLoS One Research Article The transcriptional coactivator Yorkie(Yki), is a critical downstream effector of the Hippo(Hpo) signaling pathway that controls organ size through the regulation of cell proliferation and apoptosis. During the past ten years the biological function of Yki has been studied extensively in Drosophila and a few other insects, however, little is known about it in the silkworm, Bombyx mori, a major research model of lepidopteran insect. Here, we describe the isolation, characterization and expression of the B. mori Yki ortholog, BmYki. The coding sequence of the BmYki was 1314 bp in length, encoding a protein of 437 amino acids containing two conserved WW domains. BmYki transcripts were ubiquitous but not abundant in all detected tissues and developmental stages. Comparatively, it was expressed at pretty high level in silk glands and at the stage of fifth-instar day-3 larvae. Overexpression of BmYki in cultured B. mori embryonic cells significantly promoted transcription of genes associated with cell proliferation and apoptosis, indicating that BmYki functions in the regulation of organ growth-related biological processes. Interestingly, transcription of silk protein-coding genes and transcription factors regulating the synthesis of silk proteins was downregulated remarkably, suggesting that BmYki was involved in the regulation of silk protein synthesis. This study provides new insights into the role of BmYki in Hpo pathway regulation in silkworm. Public Library of Science 2017-08-09 /pmc/articles/PMC5549978/ /pubmed/28793345 http://dx.doi.org/10.1371/journal.pone.0182690 Text en © 2017 Zeng et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zeng, Wenhui
Wang, Riyuan
Zhang, Tianyang
Gong, Chunying
Zuo, Weidong
Liu, Rongpeng
Ou, Yao
Xu, Hanfu
Cloning and expression analysis of BmYki gene in silkworm, Bombyx mori
title Cloning and expression analysis of BmYki gene in silkworm, Bombyx mori
title_full Cloning and expression analysis of BmYki gene in silkworm, Bombyx mori
title_fullStr Cloning and expression analysis of BmYki gene in silkworm, Bombyx mori
title_full_unstemmed Cloning and expression analysis of BmYki gene in silkworm, Bombyx mori
title_short Cloning and expression analysis of BmYki gene in silkworm, Bombyx mori
title_sort cloning and expression analysis of bmyki gene in silkworm, bombyx mori
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549978/
https://www.ncbi.nlm.nih.gov/pubmed/28793345
http://dx.doi.org/10.1371/journal.pone.0182690
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