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Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies
The relevance of topographic cues for commitment of induced pluripotent stem cells (iPSCs) is largely unknown. In this study, we demonstrate that groove-ridge structures with a periodicity in the submicrometer range induce elongation of iPSC colonies, guide the orientation of apical actin fibers, an...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550028/ https://www.ncbi.nlm.nih.gov/pubmed/28757164 http://dx.doi.org/10.1016/j.stemcr.2017.06.016 |
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author | Abagnale, Giulio Sechi, Antonio Steger, Michael Zhou, Qihui Kuo, Chao-Chung Aydin, Gülcan Schalla, Carmen Müller-Newen, Gerhard Zenke, Martin Costa, Ivan G. van Rijn, Patrick Gillner, Arnold Wagner, Wolfgang |
author_facet | Abagnale, Giulio Sechi, Antonio Steger, Michael Zhou, Qihui Kuo, Chao-Chung Aydin, Gülcan Schalla, Carmen Müller-Newen, Gerhard Zenke, Martin Costa, Ivan G. van Rijn, Patrick Gillner, Arnold Wagner, Wolfgang |
author_sort | Abagnale, Giulio |
collection | PubMed |
description | The relevance of topographic cues for commitment of induced pluripotent stem cells (iPSCs) is largely unknown. In this study, we demonstrate that groove-ridge structures with a periodicity in the submicrometer range induce elongation of iPSC colonies, guide the orientation of apical actin fibers, and direct the polarity of cell division. Elongation of iPSC colonies impacts also on their intrinsic molecular patterning, which seems to be orchestrated from the rim of the colonies. BMP4-induced differentiation is enhanced in elongated colonies, and the submicron grooves impact on the spatial modulation of YAP activity upon induction with this morphogen. Interestingly, TAZ, a YAP paralog, shows distinct cytoskeletal localization in iPSCs. These findings demonstrate that topography can guide orientation and organization of iPSC colonies, which may affect the interaction between mechanosensors and mechanotransducers in iPSCs. |
format | Online Article Text |
id | pubmed-5550028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-55500282017-08-17 Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies Abagnale, Giulio Sechi, Antonio Steger, Michael Zhou, Qihui Kuo, Chao-Chung Aydin, Gülcan Schalla, Carmen Müller-Newen, Gerhard Zenke, Martin Costa, Ivan G. van Rijn, Patrick Gillner, Arnold Wagner, Wolfgang Stem Cell Reports Article The relevance of topographic cues for commitment of induced pluripotent stem cells (iPSCs) is largely unknown. In this study, we demonstrate that groove-ridge structures with a periodicity in the submicrometer range induce elongation of iPSC colonies, guide the orientation of apical actin fibers, and direct the polarity of cell division. Elongation of iPSC colonies impacts also on their intrinsic molecular patterning, which seems to be orchestrated from the rim of the colonies. BMP4-induced differentiation is enhanced in elongated colonies, and the submicron grooves impact on the spatial modulation of YAP activity upon induction with this morphogen. Interestingly, TAZ, a YAP paralog, shows distinct cytoskeletal localization in iPSCs. These findings demonstrate that topography can guide orientation and organization of iPSC colonies, which may affect the interaction between mechanosensors and mechanotransducers in iPSCs. Elsevier 2017-07-27 /pmc/articles/PMC5550028/ /pubmed/28757164 http://dx.doi.org/10.1016/j.stemcr.2017.06.016 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Abagnale, Giulio Sechi, Antonio Steger, Michael Zhou, Qihui Kuo, Chao-Chung Aydin, Gülcan Schalla, Carmen Müller-Newen, Gerhard Zenke, Martin Costa, Ivan G. van Rijn, Patrick Gillner, Arnold Wagner, Wolfgang Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies |
title | Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies |
title_full | Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies |
title_fullStr | Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies |
title_full_unstemmed | Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies |
title_short | Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies |
title_sort | surface topography guides morphology and spatial patterning of induced pluripotent stem cell colonies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550028/ https://www.ncbi.nlm.nih.gov/pubmed/28757164 http://dx.doi.org/10.1016/j.stemcr.2017.06.016 |
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