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Transmission is a Noticeable Cause of Resistance Among Treated Tuberculosis Patients in Shanghai, China

It is generally believed that drug resistance among treated tuberculosis (TB) patients is as a result of acquired drug resistance due to inappropriate treatment. Previous studies have shown that primary drug resistance caused by transmission also plays a role among treated cases. Differentiating the...

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Autores principales: Nsofor, Chijioke A., Jiang, Qi, Wu, Jie, Gan, Mingyu, Liu, Qingyun, Zuo, Tianyu, Zhu, Guofeng, Gao, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550506/
https://www.ncbi.nlm.nih.gov/pubmed/28794425
http://dx.doi.org/10.1038/s41598-017-08061-3
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author Nsofor, Chijioke A.
Jiang, Qi
Wu, Jie
Gan, Mingyu
Liu, Qingyun
Zuo, Tianyu
Zhu, Guofeng
Gao, Qian
author_facet Nsofor, Chijioke A.
Jiang, Qi
Wu, Jie
Gan, Mingyu
Liu, Qingyun
Zuo, Tianyu
Zhu, Guofeng
Gao, Qian
author_sort Nsofor, Chijioke A.
collection PubMed
description It is generally believed that drug resistance among treated tuberculosis (TB) patients is as a result of acquired drug resistance due to inappropriate treatment. Previous studies have shown that primary drug resistance caused by transmission also plays a role among treated cases. Differentiating the two types of drug resistance will help in developing appropriate strategies for control of drug resistant tuberculosis. In this study, we tested the hypothesis that drug resistance among treated TB patients is mainly caused by primary resistance rather than acquired resistance. Defining resistance profiles by molecular drug susceptibility test, we used Unit Variable Number Tandem Repeats (VNTR) to genotype and Whole Genome Sequencing (WGS) to confirm the accordance of the first and last Mycobacterium tuberculosis isolates from treated pulmonary TB patients in Shanghai from 2009–2015. Among 81 patients with increasing drug resistance, out of 390 patients enrolled, paired isolates from 59.3% (48/81) had different VNTR patterns indicating primary drug resistance. Our results have demonstrated that primary resistance due to exogenous reinfection is the major cause of drug resistance among treated TB patients in Shanghai; thus, strategies aimed at preventing and interrupting transmission are urgently needed to effectively reduce the epidemic of drug resistant tuberculosis.
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spelling pubmed-55505062017-08-11 Transmission is a Noticeable Cause of Resistance Among Treated Tuberculosis Patients in Shanghai, China Nsofor, Chijioke A. Jiang, Qi Wu, Jie Gan, Mingyu Liu, Qingyun Zuo, Tianyu Zhu, Guofeng Gao, Qian Sci Rep Article It is generally believed that drug resistance among treated tuberculosis (TB) patients is as a result of acquired drug resistance due to inappropriate treatment. Previous studies have shown that primary drug resistance caused by transmission also plays a role among treated cases. Differentiating the two types of drug resistance will help in developing appropriate strategies for control of drug resistant tuberculosis. In this study, we tested the hypothesis that drug resistance among treated TB patients is mainly caused by primary resistance rather than acquired resistance. Defining resistance profiles by molecular drug susceptibility test, we used Unit Variable Number Tandem Repeats (VNTR) to genotype and Whole Genome Sequencing (WGS) to confirm the accordance of the first and last Mycobacterium tuberculosis isolates from treated pulmonary TB patients in Shanghai from 2009–2015. Among 81 patients with increasing drug resistance, out of 390 patients enrolled, paired isolates from 59.3% (48/81) had different VNTR patterns indicating primary drug resistance. Our results have demonstrated that primary resistance due to exogenous reinfection is the major cause of drug resistance among treated TB patients in Shanghai; thus, strategies aimed at preventing and interrupting transmission are urgently needed to effectively reduce the epidemic of drug resistant tuberculosis. Nature Publishing Group UK 2017-08-09 /pmc/articles/PMC5550506/ /pubmed/28794425 http://dx.doi.org/10.1038/s41598-017-08061-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nsofor, Chijioke A.
Jiang, Qi
Wu, Jie
Gan, Mingyu
Liu, Qingyun
Zuo, Tianyu
Zhu, Guofeng
Gao, Qian
Transmission is a Noticeable Cause of Resistance Among Treated Tuberculosis Patients in Shanghai, China
title Transmission is a Noticeable Cause of Resistance Among Treated Tuberculosis Patients in Shanghai, China
title_full Transmission is a Noticeable Cause of Resistance Among Treated Tuberculosis Patients in Shanghai, China
title_fullStr Transmission is a Noticeable Cause of Resistance Among Treated Tuberculosis Patients in Shanghai, China
title_full_unstemmed Transmission is a Noticeable Cause of Resistance Among Treated Tuberculosis Patients in Shanghai, China
title_short Transmission is a Noticeable Cause of Resistance Among Treated Tuberculosis Patients in Shanghai, China
title_sort transmission is a noticeable cause of resistance among treated tuberculosis patients in shanghai, china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550506/
https://www.ncbi.nlm.nih.gov/pubmed/28794425
http://dx.doi.org/10.1038/s41598-017-08061-3
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