FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis

FXYD proteins are important regulators of Na(+)/K(+)-ATPase (NKA) activity in mammals. As an inhabitant of estuaries, the pufferfish (Tetraodon nigroviridis) responds to ambient salinity changes with efficient osmoregulation, including alterations in branchial, and renal NKA activities. Previous stu...

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Autores principales: Wang, Pei-Jen, Yang, Wen-Kai, Lin, Chia-Hao, Hwang, Hau-Hsuan, Lee, Tsung-Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550679/
https://www.ncbi.nlm.nih.gov/pubmed/28848450
http://dx.doi.org/10.3389/fphys.2017.00576
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author Wang, Pei-Jen
Yang, Wen-Kai
Lin, Chia-Hao
Hwang, Hau-Hsuan
Lee, Tsung-Han
author_facet Wang, Pei-Jen
Yang, Wen-Kai
Lin, Chia-Hao
Hwang, Hau-Hsuan
Lee, Tsung-Han
author_sort Wang, Pei-Jen
collection PubMed
description FXYD proteins are important regulators of Na(+)/K(+)-ATPase (NKA) activity in mammals. As an inhabitant of estuaries, the pufferfish (Tetraodon nigroviridis) responds to ambient salinity changes with efficient osmoregulation, including alterations in branchial, and renal NKA activities. Previous studies on teleostean FXYDs have mainly focused on the expression and potential functions of FXYD proteins in gills. The goal of the present study was to elucidate the potential role of FXYD8, a member of the fish FXYD protein family, in the modulation of NKA activity in the kidneys of this euryhaline pufferfish by using molecular, biochemical, and physiological approaches. The results demonstrate that T. nigroviridis FXYD8 (TnFXYD8) interacts with NKA in renal tubules. Meanwhile, the protein expression of renal TnFXYD8 was found to be significantly upregulated in hyperosmotic seawater-acclimated pufferfish. Moreover, overexpression of TnFXYD8 in Xenopus oocytes decreased NKA activity. Our results suggest the FXYD8 is able to modulate NKA activity through inhibitory effects upon salinity challenge. The present study further extends our understanding of the functions of FXYD proteins, the regulators of NKA, in vertebrates.
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spelling pubmed-55506792017-08-28 FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis Wang, Pei-Jen Yang, Wen-Kai Lin, Chia-Hao Hwang, Hau-Hsuan Lee, Tsung-Han Front Physiol Physiology FXYD proteins are important regulators of Na(+)/K(+)-ATPase (NKA) activity in mammals. As an inhabitant of estuaries, the pufferfish (Tetraodon nigroviridis) responds to ambient salinity changes with efficient osmoregulation, including alterations in branchial, and renal NKA activities. Previous studies on teleostean FXYDs have mainly focused on the expression and potential functions of FXYD proteins in gills. The goal of the present study was to elucidate the potential role of FXYD8, a member of the fish FXYD protein family, in the modulation of NKA activity in the kidneys of this euryhaline pufferfish by using molecular, biochemical, and physiological approaches. The results demonstrate that T. nigroviridis FXYD8 (TnFXYD8) interacts with NKA in renal tubules. Meanwhile, the protein expression of renal TnFXYD8 was found to be significantly upregulated in hyperosmotic seawater-acclimated pufferfish. Moreover, overexpression of TnFXYD8 in Xenopus oocytes decreased NKA activity. Our results suggest the FXYD8 is able to modulate NKA activity through inhibitory effects upon salinity challenge. The present study further extends our understanding of the functions of FXYD proteins, the regulators of NKA, in vertebrates. Frontiers Media S.A. 2017-08-08 /pmc/articles/PMC5550679/ /pubmed/28848450 http://dx.doi.org/10.3389/fphys.2017.00576 Text en Copyright © 2017 Wang, Yang, Lin, Hwang and Lee. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Wang, Pei-Jen
Yang, Wen-Kai
Lin, Chia-Hao
Hwang, Hau-Hsuan
Lee, Tsung-Han
FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis
title FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis
title_full FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis
title_fullStr FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis
title_full_unstemmed FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis
title_short FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis
title_sort fxyd8, a novel regulator of renal na(+)/k(+)-atpase in the euryhaline teleost, tetraodon nigroviridis
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550679/
https://www.ncbi.nlm.nih.gov/pubmed/28848450
http://dx.doi.org/10.3389/fphys.2017.00576
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