FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis
FXYD proteins are important regulators of Na(+)/K(+)-ATPase (NKA) activity in mammals. As an inhabitant of estuaries, the pufferfish (Tetraodon nigroviridis) responds to ambient salinity changes with efficient osmoregulation, including alterations in branchial, and renal NKA activities. Previous stu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550679/ https://www.ncbi.nlm.nih.gov/pubmed/28848450 http://dx.doi.org/10.3389/fphys.2017.00576 |
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author | Wang, Pei-Jen Yang, Wen-Kai Lin, Chia-Hao Hwang, Hau-Hsuan Lee, Tsung-Han |
author_facet | Wang, Pei-Jen Yang, Wen-Kai Lin, Chia-Hao Hwang, Hau-Hsuan Lee, Tsung-Han |
author_sort | Wang, Pei-Jen |
collection | PubMed |
description | FXYD proteins are important regulators of Na(+)/K(+)-ATPase (NKA) activity in mammals. As an inhabitant of estuaries, the pufferfish (Tetraodon nigroviridis) responds to ambient salinity changes with efficient osmoregulation, including alterations in branchial, and renal NKA activities. Previous studies on teleostean FXYDs have mainly focused on the expression and potential functions of FXYD proteins in gills. The goal of the present study was to elucidate the potential role of FXYD8, a member of the fish FXYD protein family, in the modulation of NKA activity in the kidneys of this euryhaline pufferfish by using molecular, biochemical, and physiological approaches. The results demonstrate that T. nigroviridis FXYD8 (TnFXYD8) interacts with NKA in renal tubules. Meanwhile, the protein expression of renal TnFXYD8 was found to be significantly upregulated in hyperosmotic seawater-acclimated pufferfish. Moreover, overexpression of TnFXYD8 in Xenopus oocytes decreased NKA activity. Our results suggest the FXYD8 is able to modulate NKA activity through inhibitory effects upon salinity challenge. The present study further extends our understanding of the functions of FXYD proteins, the regulators of NKA, in vertebrates. |
format | Online Article Text |
id | pubmed-5550679 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55506792017-08-28 FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis Wang, Pei-Jen Yang, Wen-Kai Lin, Chia-Hao Hwang, Hau-Hsuan Lee, Tsung-Han Front Physiol Physiology FXYD proteins are important regulators of Na(+)/K(+)-ATPase (NKA) activity in mammals. As an inhabitant of estuaries, the pufferfish (Tetraodon nigroviridis) responds to ambient salinity changes with efficient osmoregulation, including alterations in branchial, and renal NKA activities. Previous studies on teleostean FXYDs have mainly focused on the expression and potential functions of FXYD proteins in gills. The goal of the present study was to elucidate the potential role of FXYD8, a member of the fish FXYD protein family, in the modulation of NKA activity in the kidneys of this euryhaline pufferfish by using molecular, biochemical, and physiological approaches. The results demonstrate that T. nigroviridis FXYD8 (TnFXYD8) interacts with NKA in renal tubules. Meanwhile, the protein expression of renal TnFXYD8 was found to be significantly upregulated in hyperosmotic seawater-acclimated pufferfish. Moreover, overexpression of TnFXYD8 in Xenopus oocytes decreased NKA activity. Our results suggest the FXYD8 is able to modulate NKA activity through inhibitory effects upon salinity challenge. The present study further extends our understanding of the functions of FXYD proteins, the regulators of NKA, in vertebrates. Frontiers Media S.A. 2017-08-08 /pmc/articles/PMC5550679/ /pubmed/28848450 http://dx.doi.org/10.3389/fphys.2017.00576 Text en Copyright © 2017 Wang, Yang, Lin, Hwang and Lee. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Wang, Pei-Jen Yang, Wen-Kai Lin, Chia-Hao Hwang, Hau-Hsuan Lee, Tsung-Han FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis |
title | FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis |
title_full | FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis |
title_fullStr | FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis |
title_full_unstemmed | FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis |
title_short | FXYD8, a Novel Regulator of Renal Na(+)/K(+)-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis |
title_sort | fxyd8, a novel regulator of renal na(+)/k(+)-atpase in the euryhaline teleost, tetraodon nigroviridis |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550679/ https://www.ncbi.nlm.nih.gov/pubmed/28848450 http://dx.doi.org/10.3389/fphys.2017.00576 |
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