Cargando…

Tethered Magnets Are the Key to Magnetotaxis: Direct Observations of Magnetospirillum magneticum AMB-1 Show that MamK Distributes Magnetosome Organelles Equally to Daughter Cells

Magnetotactic bacteria are a unique group of bacteria that synthesize a magnetic organelle termed the magnetosome, which they use to assist with their magnetic navigation in a specific type of bacterial motility called magneto-aerotaxis. Cytoskeletal filaments consisting of the actin-like protein Ma...

Descripción completa

Detalles Bibliográficos
Autores principales: Taoka, Azuma, Kiyokawa, Ayako, Uesugi, Chika, Kikuchi, Yousuke, Oestreicher, Zachery, Morii, Kaori, Eguchi, Yukako, Fukumori, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550748/
https://www.ncbi.nlm.nih.gov/pubmed/28790202
http://dx.doi.org/10.1128/mBio.00679-17
_version_ 1783256177169137664
author Taoka, Azuma
Kiyokawa, Ayako
Uesugi, Chika
Kikuchi, Yousuke
Oestreicher, Zachery
Morii, Kaori
Eguchi, Yukako
Fukumori, Yoshihiro
author_facet Taoka, Azuma
Kiyokawa, Ayako
Uesugi, Chika
Kikuchi, Yousuke
Oestreicher, Zachery
Morii, Kaori
Eguchi, Yukako
Fukumori, Yoshihiro
author_sort Taoka, Azuma
collection PubMed
description Magnetotactic bacteria are a unique group of bacteria that synthesize a magnetic organelle termed the magnetosome, which they use to assist with their magnetic navigation in a specific type of bacterial motility called magneto-aerotaxis. Cytoskeletal filaments consisting of the actin-like protein MamK are associated with the magnetosome chain. Previously, the function of MamK was thought to be in positioning magnetosome organelles; this was proposed based on observations via electron microscopy still images. Here, we conducted live-cell time-lapse fluorescence imaging analyses employing highly inclined and laminated optical sheet microscopy, and these methods enabled us to visualize detailed dynamic movement of magnetosomes in growing cells during the entire cell cycle with high-temporal resolution and a high signal/noise ratio. We found that the MamK cytoskeleton anchors magnetosomes through a mechanism that requires MamK-ATPase activity throughout the cell cycle to prevent simple diffusion of magnetosomes within the cell. We concluded that the static chain-like arrangement of the magnetosomes is required to precisely and consistently segregate the magnetosomes to daughter cells. Thus, the daughter cells inherit a functional magnetic sensor that mediates magneto-reception.
format Online
Article
Text
id pubmed-5550748
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-55507482017-08-14 Tethered Magnets Are the Key to Magnetotaxis: Direct Observations of Magnetospirillum magneticum AMB-1 Show that MamK Distributes Magnetosome Organelles Equally to Daughter Cells Taoka, Azuma Kiyokawa, Ayako Uesugi, Chika Kikuchi, Yousuke Oestreicher, Zachery Morii, Kaori Eguchi, Yukako Fukumori, Yoshihiro mBio Research Article Magnetotactic bacteria are a unique group of bacteria that synthesize a magnetic organelle termed the magnetosome, which they use to assist with their magnetic navigation in a specific type of bacterial motility called magneto-aerotaxis. Cytoskeletal filaments consisting of the actin-like protein MamK are associated with the magnetosome chain. Previously, the function of MamK was thought to be in positioning magnetosome organelles; this was proposed based on observations via electron microscopy still images. Here, we conducted live-cell time-lapse fluorescence imaging analyses employing highly inclined and laminated optical sheet microscopy, and these methods enabled us to visualize detailed dynamic movement of magnetosomes in growing cells during the entire cell cycle with high-temporal resolution and a high signal/noise ratio. We found that the MamK cytoskeleton anchors magnetosomes through a mechanism that requires MamK-ATPase activity throughout the cell cycle to prevent simple diffusion of magnetosomes within the cell. We concluded that the static chain-like arrangement of the magnetosomes is required to precisely and consistently segregate the magnetosomes to daughter cells. Thus, the daughter cells inherit a functional magnetic sensor that mediates magneto-reception. American Society for Microbiology 2017-08-08 /pmc/articles/PMC5550748/ /pubmed/28790202 http://dx.doi.org/10.1128/mBio.00679-17 Text en Copyright © 2017 Taoka et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Taoka, Azuma
Kiyokawa, Ayako
Uesugi, Chika
Kikuchi, Yousuke
Oestreicher, Zachery
Morii, Kaori
Eguchi, Yukako
Fukumori, Yoshihiro
Tethered Magnets Are the Key to Magnetotaxis: Direct Observations of Magnetospirillum magneticum AMB-1 Show that MamK Distributes Magnetosome Organelles Equally to Daughter Cells
title Tethered Magnets Are the Key to Magnetotaxis: Direct Observations of Magnetospirillum magneticum AMB-1 Show that MamK Distributes Magnetosome Organelles Equally to Daughter Cells
title_full Tethered Magnets Are the Key to Magnetotaxis: Direct Observations of Magnetospirillum magneticum AMB-1 Show that MamK Distributes Magnetosome Organelles Equally to Daughter Cells
title_fullStr Tethered Magnets Are the Key to Magnetotaxis: Direct Observations of Magnetospirillum magneticum AMB-1 Show that MamK Distributes Magnetosome Organelles Equally to Daughter Cells
title_full_unstemmed Tethered Magnets Are the Key to Magnetotaxis: Direct Observations of Magnetospirillum magneticum AMB-1 Show that MamK Distributes Magnetosome Organelles Equally to Daughter Cells
title_short Tethered Magnets Are the Key to Magnetotaxis: Direct Observations of Magnetospirillum magneticum AMB-1 Show that MamK Distributes Magnetosome Organelles Equally to Daughter Cells
title_sort tethered magnets are the key to magnetotaxis: direct observations of magnetospirillum magneticum amb-1 show that mamk distributes magnetosome organelles equally to daughter cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550748/
https://www.ncbi.nlm.nih.gov/pubmed/28790202
http://dx.doi.org/10.1128/mBio.00679-17
work_keys_str_mv AT taokaazuma tetheredmagnetsarethekeytomagnetotaxisdirectobservationsofmagnetospirillummagneticumamb1showthatmamkdistributesmagnetosomeorganellesequallytodaughtercells
AT kiyokawaayako tetheredmagnetsarethekeytomagnetotaxisdirectobservationsofmagnetospirillummagneticumamb1showthatmamkdistributesmagnetosomeorganellesequallytodaughtercells
AT uesugichika tetheredmagnetsarethekeytomagnetotaxisdirectobservationsofmagnetospirillummagneticumamb1showthatmamkdistributesmagnetosomeorganellesequallytodaughtercells
AT kikuchiyousuke tetheredmagnetsarethekeytomagnetotaxisdirectobservationsofmagnetospirillummagneticumamb1showthatmamkdistributesmagnetosomeorganellesequallytodaughtercells
AT oestreicherzachery tetheredmagnetsarethekeytomagnetotaxisdirectobservationsofmagnetospirillummagneticumamb1showthatmamkdistributesmagnetosomeorganellesequallytodaughtercells
AT moriikaori tetheredmagnetsarethekeytomagnetotaxisdirectobservationsofmagnetospirillummagneticumamb1showthatmamkdistributesmagnetosomeorganellesequallytodaughtercells
AT eguchiyukako tetheredmagnetsarethekeytomagnetotaxisdirectobservationsofmagnetospirillummagneticumamb1showthatmamkdistributesmagnetosomeorganellesequallytodaughtercells
AT fukumoriyoshihiro tetheredmagnetsarethekeytomagnetotaxisdirectobservationsofmagnetospirillummagneticumamb1showthatmamkdistributesmagnetosomeorganellesequallytodaughtercells