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Pleiotropic effects of sphingosine-1-phosphate signaling to control human chorionic mesenchymal stem cell physiology

Chorionic stem cells represent a promising opportunity for regenerative medicine. A deeper understanding of the stimuli that regulate their physiology, could lead to innovative clinical approaches. We revealed the presence of multiple sphingosine-1-phosphate (S1P) receptor isoforms in chorion-derive...

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Autores principales: Innamorati, Giulio, Fontana, Emanuela, Steccanella, Federica, Gandhi, Kushal, Bassi, Giulio, Zandonà, Valeria, Giacomello, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550859/
https://www.ncbi.nlm.nih.gov/pubmed/28703804
http://dx.doi.org/10.1038/cddis.2017.312
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author Innamorati, Giulio
Fontana, Emanuela
Steccanella, Federica
Gandhi, Kushal
Bassi, Giulio
Zandonà, Valeria
Giacomello, Luca
author_facet Innamorati, Giulio
Fontana, Emanuela
Steccanella, Federica
Gandhi, Kushal
Bassi, Giulio
Zandonà, Valeria
Giacomello, Luca
author_sort Innamorati, Giulio
collection PubMed
description Chorionic stem cells represent a promising opportunity for regenerative medicine. A deeper understanding of the stimuli that regulate their physiology, could lead to innovative clinical approaches. We revealed the presence of multiple sphingosine-1-phosphate (S1P) receptor isoforms in chorion-derived mesenchymal stem cells (CMSCs). Their activation simultaneously propagated from the plasma membrane through Gi and other heterotrimeric G proteins and further diverged toward extracellular-signal-regulated kinase 1/2 (ERK1/2), p38 and protein kinase D 1. At a functional level, S1P signaling inhibited CMSC migration, while promoting proliferation. Instead, a reduction of cell density was obtained when S1P was combined to treatments that increased cAMP intracellular concentration. Such surprising reduction of cell viability was relatively specific as it was not observed with stromal stem cells from bone marrow. Neither it was observed by activating analogous G proteins with bradykinin nor by inducing cell death via a cAMP-independent pathway. S1P could thus reveal novel keys to improve CMSC differentiation programs acting on cAMP concentration. Furthermore, S1P receptor agonists/antagonists could become instrumental in favoring CMSC engraftment by controlling cell motility.
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spelling pubmed-55508592017-08-14 Pleiotropic effects of sphingosine-1-phosphate signaling to control human chorionic mesenchymal stem cell physiology Innamorati, Giulio Fontana, Emanuela Steccanella, Federica Gandhi, Kushal Bassi, Giulio Zandonà, Valeria Giacomello, Luca Cell Death Dis Original Article Chorionic stem cells represent a promising opportunity for regenerative medicine. A deeper understanding of the stimuli that regulate their physiology, could lead to innovative clinical approaches. We revealed the presence of multiple sphingosine-1-phosphate (S1P) receptor isoforms in chorion-derived mesenchymal stem cells (CMSCs). Their activation simultaneously propagated from the plasma membrane through Gi and other heterotrimeric G proteins and further diverged toward extracellular-signal-regulated kinase 1/2 (ERK1/2), p38 and protein kinase D 1. At a functional level, S1P signaling inhibited CMSC migration, while promoting proliferation. Instead, a reduction of cell density was obtained when S1P was combined to treatments that increased cAMP intracellular concentration. Such surprising reduction of cell viability was relatively specific as it was not observed with stromal stem cells from bone marrow. Neither it was observed by activating analogous G proteins with bradykinin nor by inducing cell death via a cAMP-independent pathway. S1P could thus reveal novel keys to improve CMSC differentiation programs acting on cAMP concentration. Furthermore, S1P receptor agonists/antagonists could become instrumental in favoring CMSC engraftment by controlling cell motility. Nature Publishing Group 2017-07 2017-07-13 /pmc/articles/PMC5550859/ /pubmed/28703804 http://dx.doi.org/10.1038/cddis.2017.312 Text en Copyright © 2017 The Author(s) http://creativecommons.org/licenses/by/4.0/ Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Original Article
Innamorati, Giulio
Fontana, Emanuela
Steccanella, Federica
Gandhi, Kushal
Bassi, Giulio
Zandonà, Valeria
Giacomello, Luca
Pleiotropic effects of sphingosine-1-phosphate signaling to control human chorionic mesenchymal stem cell physiology
title Pleiotropic effects of sphingosine-1-phosphate signaling to control human chorionic mesenchymal stem cell physiology
title_full Pleiotropic effects of sphingosine-1-phosphate signaling to control human chorionic mesenchymal stem cell physiology
title_fullStr Pleiotropic effects of sphingosine-1-phosphate signaling to control human chorionic mesenchymal stem cell physiology
title_full_unstemmed Pleiotropic effects of sphingosine-1-phosphate signaling to control human chorionic mesenchymal stem cell physiology
title_short Pleiotropic effects of sphingosine-1-phosphate signaling to control human chorionic mesenchymal stem cell physiology
title_sort pleiotropic effects of sphingosine-1-phosphate signaling to control human chorionic mesenchymal stem cell physiology
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550859/
https://www.ncbi.nlm.nih.gov/pubmed/28703804
http://dx.doi.org/10.1038/cddis.2017.312
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