Cargando…
Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples
This study presents a comprehensive approach of a clonal diversity analysis of 448 Extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from environmental, human and food samples in Spain. The phenotypic confirmation of ESBL production was performed by disc diffusion and microdilution met...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551114/ https://www.ncbi.nlm.nih.gov/pubmed/28644413 http://dx.doi.org/10.3390/ijerph14070676 |
_version_ | 1783256244977401856 |
---|---|
author | Ojer-Usoz, Elena González, David Vitas, Ana Isabel |
author_facet | Ojer-Usoz, Elena González, David Vitas, Ana Isabel |
author_sort | Ojer-Usoz, Elena |
collection | PubMed |
description | This study presents a comprehensive approach of a clonal diversity analysis of 448 Extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from environmental, human and food samples in Spain. The phenotypic confirmation of ESBL production was performed by disc diffusion and microdilution methods, while Polymerase Chain Reaction (PCR) and sequencing were used for the molecular characterization of β-lactamase genes (bla(CTX-M), bla(SHV), bla(TEM), bla(OXA)). Clonal relationship of isolates was determined by multi-locus sequence typing (MLST). Multidrug resistant strains were present in all the studied niches, with percentages above 50.0%. The most prevalent β-lactamase genes were bla(CTXM-14) (26%) and bla(CTXM-1) (21.4%), followed by bla(SHV-12), bla(CTX-M-15) and bla(TEM-42). MLST isolates were grouped into 26 clonal complexes (CC) and 177 different sequence types (ST) were detected. Despite the high clonal diversity observed, CC10 was the prevalent and the only CC detected in all niches, while other complexes as CC131 were mainly associated to human isolates. The observed prevalence and diversity of these resistant bacteria across the different environments encourages a One Health approach to prevent and control ESBL dissemination between environment and consumers. |
format | Online Article Text |
id | pubmed-5551114 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-55511142017-08-11 Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples Ojer-Usoz, Elena González, David Vitas, Ana Isabel Int J Environ Res Public Health Article This study presents a comprehensive approach of a clonal diversity analysis of 448 Extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from environmental, human and food samples in Spain. The phenotypic confirmation of ESBL production was performed by disc diffusion and microdilution methods, while Polymerase Chain Reaction (PCR) and sequencing were used for the molecular characterization of β-lactamase genes (bla(CTX-M), bla(SHV), bla(TEM), bla(OXA)). Clonal relationship of isolates was determined by multi-locus sequence typing (MLST). Multidrug resistant strains were present in all the studied niches, with percentages above 50.0%. The most prevalent β-lactamase genes were bla(CTXM-14) (26%) and bla(CTXM-1) (21.4%), followed by bla(SHV-12), bla(CTX-M-15) and bla(TEM-42). MLST isolates were grouped into 26 clonal complexes (CC) and 177 different sequence types (ST) were detected. Despite the high clonal diversity observed, CC10 was the prevalent and the only CC detected in all niches, while other complexes as CC131 were mainly associated to human isolates. The observed prevalence and diversity of these resistant bacteria across the different environments encourages a One Health approach to prevent and control ESBL dissemination between environment and consumers. MDPI 2017-06-23 2017-07 /pmc/articles/PMC5551114/ /pubmed/28644413 http://dx.doi.org/10.3390/ijerph14070676 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ojer-Usoz, Elena González, David Vitas, Ana Isabel Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples |
title | Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples |
title_full | Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples |
title_fullStr | Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples |
title_full_unstemmed | Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples |
title_short | Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples |
title_sort | clonal diversity of esbl-producing escherichia coli isolated from environmental, human and food samples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551114/ https://www.ncbi.nlm.nih.gov/pubmed/28644413 http://dx.doi.org/10.3390/ijerph14070676 |
work_keys_str_mv | AT ojerusozelena clonaldiversityofesblproducingescherichiacoliisolatedfromenvironmentalhumanandfoodsamples AT gonzalezdavid clonaldiversityofesblproducingescherichiacoliisolatedfromenvironmentalhumanandfoodsamples AT vitasanaisabel clonaldiversityofesblproducingescherichiacoliisolatedfromenvironmentalhumanandfoodsamples |