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Determination and Tissue Distribution Comparisons of Atractylodin after Oral Administration of Crude and Processed Atractylodes Rhizome

BACKGROUND: Atractylodis rhizoma is one of the most often used drugs in traditional Chinese medicine. Stir frying with wheat bran is the most common processing method. To clarify the principle of processing, an experiment was carried out to compare the tissue distribution of typical constituent afte...

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Autores principales: Liu, Yang-Zhi, Liu, Yu-Qiang, Jia, Rui, Li, Jun, Chang, Xiao-Wen, Xu, Chen-Xi, Cai, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551358/
https://www.ncbi.nlm.nih.gov/pubmed/28839365
http://dx.doi.org/10.4103/pm.pm_394_16
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author Liu, Yang-Zhi
Liu, Yu-Qiang
Jia, Rui
Li, Jun
Chang, Xiao-Wen
Xu, Chen-Xi
Cai, Qian
author_facet Liu, Yang-Zhi
Liu, Yu-Qiang
Jia, Rui
Li, Jun
Chang, Xiao-Wen
Xu, Chen-Xi
Cai, Qian
author_sort Liu, Yang-Zhi
collection PubMed
description BACKGROUND: Atractylodis rhizoma is one of the most often used drugs in traditional Chinese medicine. Stir frying with wheat bran is the most common processing method. To clarify the principle of processing, an experiment was carried out to compare the tissue distribution of typical constituent after oral administration of raw A. rhizoma and processed ones. OBJECTIVE: To compare the tissues distribution of atractylodin after oral administration of raw and processed A. rhizoma and clarify the processing principle of A. rhizoma. Materials and Methods: High-performance liquid chromatogram with ultraviolet detection was developed and validated for the determination of atractylodin in rat tissues. RESULTS: The atractylodin in raw and processed A. rhizoma was distributed in all tissues involved in this study. CONCLUSIONS: The concentration of atractylodin in it is the highest in the stomach and small intestine. SUMMARY: In this paper, a simple, specific, and rapid reversed phase-high-performance liquid chromatogram method with ultraviolet detection for quantification of atractylodin in rat tissue has been developed for the first time. The result indicates that the concentration of atractylodin in it is the highest in the stomach and small intestine. Abbreviations used: IS: Internal standard substance; A. rhizoma: Atractylodis rhizoma; RSD: Relative standard deviation; HPLC: High performance liquid chromatography.
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spelling pubmed-55513582017-08-24 Determination and Tissue Distribution Comparisons of Atractylodin after Oral Administration of Crude and Processed Atractylodes Rhizome Liu, Yang-Zhi Liu, Yu-Qiang Jia, Rui Li, Jun Chang, Xiao-Wen Xu, Chen-Xi Cai, Qian Pharmacogn Mag Original Article BACKGROUND: Atractylodis rhizoma is one of the most often used drugs in traditional Chinese medicine. Stir frying with wheat bran is the most common processing method. To clarify the principle of processing, an experiment was carried out to compare the tissue distribution of typical constituent after oral administration of raw A. rhizoma and processed ones. OBJECTIVE: To compare the tissues distribution of atractylodin after oral administration of raw and processed A. rhizoma and clarify the processing principle of A. rhizoma. Materials and Methods: High-performance liquid chromatogram with ultraviolet detection was developed and validated for the determination of atractylodin in rat tissues. RESULTS: The atractylodin in raw and processed A. rhizoma was distributed in all tissues involved in this study. CONCLUSIONS: The concentration of atractylodin in it is the highest in the stomach and small intestine. SUMMARY: In this paper, a simple, specific, and rapid reversed phase-high-performance liquid chromatogram method with ultraviolet detection for quantification of atractylodin in rat tissue has been developed for the first time. The result indicates that the concentration of atractylodin in it is the highest in the stomach and small intestine. Abbreviations used: IS: Internal standard substance; A. rhizoma: Atractylodis rhizoma; RSD: Relative standard deviation; HPLC: High performance liquid chromatography. Medknow Publications & Media Pvt Ltd 2017 2017-07-19 /pmc/articles/PMC5551358/ /pubmed/28839365 http://dx.doi.org/10.4103/pm.pm_394_16 Text en Copyright: © 2017 Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Liu, Yang-Zhi
Liu, Yu-Qiang
Jia, Rui
Li, Jun
Chang, Xiao-Wen
Xu, Chen-Xi
Cai, Qian
Determination and Tissue Distribution Comparisons of Atractylodin after Oral Administration of Crude and Processed Atractylodes Rhizome
title Determination and Tissue Distribution Comparisons of Atractylodin after Oral Administration of Crude and Processed Atractylodes Rhizome
title_full Determination and Tissue Distribution Comparisons of Atractylodin after Oral Administration of Crude and Processed Atractylodes Rhizome
title_fullStr Determination and Tissue Distribution Comparisons of Atractylodin after Oral Administration of Crude and Processed Atractylodes Rhizome
title_full_unstemmed Determination and Tissue Distribution Comparisons of Atractylodin after Oral Administration of Crude and Processed Atractylodes Rhizome
title_short Determination and Tissue Distribution Comparisons of Atractylodin after Oral Administration of Crude and Processed Atractylodes Rhizome
title_sort determination and tissue distribution comparisons of atractylodin after oral administration of crude and processed atractylodes rhizome
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551358/
https://www.ncbi.nlm.nih.gov/pubmed/28839365
http://dx.doi.org/10.4103/pm.pm_394_16
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