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A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase

In mitosis, cells undergo a precisely orchestrated series of spatiotemporal changes in cytoskeletal structure to divide their genetic material. These changes are coordinated by a sophisticated network of protein–protein interactions and posttranslational modifications. In this study, we report a bif...

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Autores principales: Cullati, Sierra N., Kabeche, Lilian, Kettenbach, Arminja N., Gerber, Scott A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551695/
https://www.ncbi.nlm.nih.gov/pubmed/28630147
http://dx.doi.org/10.1083/jcb.201512055
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author Cullati, Sierra N.
Kabeche, Lilian
Kettenbach, Arminja N.
Gerber, Scott A.
author_facet Cullati, Sierra N.
Kabeche, Lilian
Kettenbach, Arminja N.
Gerber, Scott A.
author_sort Cullati, Sierra N.
collection PubMed
description In mitosis, cells undergo a precisely orchestrated series of spatiotemporal changes in cytoskeletal structure to divide their genetic material. These changes are coordinated by a sophisticated network of protein–protein interactions and posttranslational modifications. In this study, we report a bifurcation in a signaling cascade of the NIMA-related kinases (Neks) Nek6, Nek7, and Nek9 that is required for the localization and function of two kinesins essential for cytokinesis, Mklp2 and Kif14. We demonstrate that a Nek9, Nek6, and Mklp2 signaling module controls the timely localization and bundling activity of Mklp2 at the anaphase central spindle. We further show that a separate Nek9, Nek7, and Kif14 signaling module is required for the recruitment of the Rho-interacting kinase citron to the anaphase midzone. Our findings uncover an anaphase-specific function for these effector kinesins that is controlled by specific Nek kinase signaling modules to properly coordinate cytokinesis.
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spelling pubmed-55516952018-02-07 A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase Cullati, Sierra N. Kabeche, Lilian Kettenbach, Arminja N. Gerber, Scott A. J Cell Biol Research Articles In mitosis, cells undergo a precisely orchestrated series of spatiotemporal changes in cytoskeletal structure to divide their genetic material. These changes are coordinated by a sophisticated network of protein–protein interactions and posttranslational modifications. In this study, we report a bifurcation in a signaling cascade of the NIMA-related kinases (Neks) Nek6, Nek7, and Nek9 that is required for the localization and function of two kinesins essential for cytokinesis, Mklp2 and Kif14. We demonstrate that a Nek9, Nek6, and Mklp2 signaling module controls the timely localization and bundling activity of Mklp2 at the anaphase central spindle. We further show that a separate Nek9, Nek7, and Kif14 signaling module is required for the recruitment of the Rho-interacting kinase citron to the anaphase midzone. Our findings uncover an anaphase-specific function for these effector kinesins that is controlled by specific Nek kinase signaling modules to properly coordinate cytokinesis. The Rockefeller University Press 2017-08-07 /pmc/articles/PMC5551695/ /pubmed/28630147 http://dx.doi.org/10.1083/jcb.201512055 Text en © 2017 Cullati et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Cullati, Sierra N.
Kabeche, Lilian
Kettenbach, Arminja N.
Gerber, Scott A.
A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase
title A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase
title_full A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase
title_fullStr A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase
title_full_unstemmed A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase
title_short A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase
title_sort bifurcated signaling cascade of nima-related kinases controls distinct kinesins in anaphase
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551695/
https://www.ncbi.nlm.nih.gov/pubmed/28630147
http://dx.doi.org/10.1083/jcb.201512055
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