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F-actin prevents interaction between sperm DNA and the oocyte meiotic spindle in C. elegans
Fertilization occurs during female meiosis in most animals, which raises the question of what prevents the sperm DNA from interacting with the meiotic spindle. In this study, we find that Caenorhabditis elegans sperm DNA stays in a fixed position at the opposite end of the embryo from the meiotic sp...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551714/ https://www.ncbi.nlm.nih.gov/pubmed/28637747 http://dx.doi.org/10.1083/jcb.201702020 |
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author | Panzica, Michelle T. Marin, Harold C. Reymann, Anne-Cecile McNally, Francis J. |
author_facet | Panzica, Michelle T. Marin, Harold C. Reymann, Anne-Cecile McNally, Francis J. |
author_sort | Panzica, Michelle T. |
collection | PubMed |
description | Fertilization occurs during female meiosis in most animals, which raises the question of what prevents the sperm DNA from interacting with the meiotic spindle. In this study, we find that Caenorhabditis elegans sperm DNA stays in a fixed position at the opposite end of the embryo from the meiotic spindle while yolk granules are transported throughout the embryo by kinesin-1. In the absence of F-actin, the sperm DNA, centrioles, and organelles were transported as a unit with the yolk granules, resulting in sperm DNA within 2 µm of the meiotic spindle. F-actin imaging revealed a cytoplasmic meshwork that might restrict transport in a size-dependent manner. However, increasing yolk granule size did not slow their velocity, and the F-actin moved with the yolk granules. Instead, sperm contents connect to the cortical F-actin to prevent interaction with the meiotic spindle. |
format | Online Article Text |
id | pubmed-5551714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-55517142018-02-07 F-actin prevents interaction between sperm DNA and the oocyte meiotic spindle in C. elegans Panzica, Michelle T. Marin, Harold C. Reymann, Anne-Cecile McNally, Francis J. J Cell Biol Research Articles Fertilization occurs during female meiosis in most animals, which raises the question of what prevents the sperm DNA from interacting with the meiotic spindle. In this study, we find that Caenorhabditis elegans sperm DNA stays in a fixed position at the opposite end of the embryo from the meiotic spindle while yolk granules are transported throughout the embryo by kinesin-1. In the absence of F-actin, the sperm DNA, centrioles, and organelles were transported as a unit with the yolk granules, resulting in sperm DNA within 2 µm of the meiotic spindle. F-actin imaging revealed a cytoplasmic meshwork that might restrict transport in a size-dependent manner. However, increasing yolk granule size did not slow their velocity, and the F-actin moved with the yolk granules. Instead, sperm contents connect to the cortical F-actin to prevent interaction with the meiotic spindle. The Rockefeller University Press 2017-08-07 /pmc/articles/PMC5551714/ /pubmed/28637747 http://dx.doi.org/10.1083/jcb.201702020 Text en © 2017 Panzica et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Panzica, Michelle T. Marin, Harold C. Reymann, Anne-Cecile McNally, Francis J. F-actin prevents interaction between sperm DNA and the oocyte meiotic spindle in C. elegans |
title | F-actin prevents interaction between sperm DNA and the oocyte meiotic spindle in C. elegans |
title_full | F-actin prevents interaction between sperm DNA and the oocyte meiotic spindle in C. elegans |
title_fullStr | F-actin prevents interaction between sperm DNA and the oocyte meiotic spindle in C. elegans |
title_full_unstemmed | F-actin prevents interaction between sperm DNA and the oocyte meiotic spindle in C. elegans |
title_short | F-actin prevents interaction between sperm DNA and the oocyte meiotic spindle in C. elegans |
title_sort | f-actin prevents interaction between sperm dna and the oocyte meiotic spindle in c. elegans |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551714/ https://www.ncbi.nlm.nih.gov/pubmed/28637747 http://dx.doi.org/10.1083/jcb.201702020 |
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