Cargando…
Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications
Biosynthetic materials have emerged as one of the most exciting and productive fields in polymer chemistry due to their widespread adoption and potential applications in tissue engineering (TE) research. In this work, we report the synthesis of a poly(ε-caprolactone)-graft-collagen (PCL-g-Coll) copo...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551736/ https://www.ncbi.nlm.nih.gov/pubmed/28773053 http://dx.doi.org/10.3390/ma10070693 |
_version_ | 1783256342369140736 |
---|---|
author | Gentile, Piergiorgio McColgan-Bannon, Kegan Gianone, Nicolò Ceretto Sefat, Farshid Dalgarno, Kenneth Ferreira, Ana Marina |
author_facet | Gentile, Piergiorgio McColgan-Bannon, Kegan Gianone, Nicolò Ceretto Sefat, Farshid Dalgarno, Kenneth Ferreira, Ana Marina |
author_sort | Gentile, Piergiorgio |
collection | PubMed |
description | Biosynthetic materials have emerged as one of the most exciting and productive fields in polymer chemistry due to their widespread adoption and potential applications in tissue engineering (TE) research. In this work, we report the synthesis of a poly(ε-caprolactone)-graft-collagen (PCL-g-Coll) copolymer. We combine its good mechanical and biodegradable PCL properties with the great biological properties of type I collagen as a functional material for TE. PCL, previously dissolved in dimethylformamide/dichloromethane mixture, and reacted with collagen using carbodiimide coupling chemistry. The synthesised material was characterised physically, chemically and biologically, using pure PCL and PCL/Coll blend samples as control. Infrared spectroscopy evidenced the presence of amide I and II peaks for the conjugated material. Similarly, XPS evidenced the presence of C–N and N–C=O bonds (8.96 ± 2.02% and 8.52 ± 0.63%; respectively) for PCL-g-Coll. Static contact angles showed a slight decrease in the conjugated sample. However, good biocompatibility and metabolic activity was obtained on PCL-g-Coll films compared to PCL and blend controls. After 3 days of culture, fibroblasts exhibited a spindle-like morphology, spreading homogeneously along the PCL-g-Coll film surface. We have engineered a functional biosynthetic polymer that can be processed by electrospinning. |
format | Online Article Text |
id | pubmed-5551736 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-55517362017-08-11 Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications Gentile, Piergiorgio McColgan-Bannon, Kegan Gianone, Nicolò Ceretto Sefat, Farshid Dalgarno, Kenneth Ferreira, Ana Marina Materials (Basel) Article Biosynthetic materials have emerged as one of the most exciting and productive fields in polymer chemistry due to their widespread adoption and potential applications in tissue engineering (TE) research. In this work, we report the synthesis of a poly(ε-caprolactone)-graft-collagen (PCL-g-Coll) copolymer. We combine its good mechanical and biodegradable PCL properties with the great biological properties of type I collagen as a functional material for TE. PCL, previously dissolved in dimethylformamide/dichloromethane mixture, and reacted with collagen using carbodiimide coupling chemistry. The synthesised material was characterised physically, chemically and biologically, using pure PCL and PCL/Coll blend samples as control. Infrared spectroscopy evidenced the presence of amide I and II peaks for the conjugated material. Similarly, XPS evidenced the presence of C–N and N–C=O bonds (8.96 ± 2.02% and 8.52 ± 0.63%; respectively) for PCL-g-Coll. Static contact angles showed a slight decrease in the conjugated sample. However, good biocompatibility and metabolic activity was obtained on PCL-g-Coll films compared to PCL and blend controls. After 3 days of culture, fibroblasts exhibited a spindle-like morphology, spreading homogeneously along the PCL-g-Coll film surface. We have engineered a functional biosynthetic polymer that can be processed by electrospinning. MDPI 2017-06-23 /pmc/articles/PMC5551736/ /pubmed/28773053 http://dx.doi.org/10.3390/ma10070693 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gentile, Piergiorgio McColgan-Bannon, Kegan Gianone, Nicolò Ceretto Sefat, Farshid Dalgarno, Kenneth Ferreira, Ana Marina Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications |
title | Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications |
title_full | Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications |
title_fullStr | Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications |
title_full_unstemmed | Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications |
title_short | Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications |
title_sort | biosynthetic pcl-graft-collagen bulk material for tissue engineering applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551736/ https://www.ncbi.nlm.nih.gov/pubmed/28773053 http://dx.doi.org/10.3390/ma10070693 |
work_keys_str_mv | AT gentilepiergiorgio biosyntheticpclgraftcollagenbulkmaterialfortissueengineeringapplications AT mccolganbannonkegan biosyntheticpclgraftcollagenbulkmaterialfortissueengineeringapplications AT gianonenicoloceretto biosyntheticpclgraftcollagenbulkmaterialfortissueengineeringapplications AT sefatfarshid biosyntheticpclgraftcollagenbulkmaterialfortissueengineeringapplications AT dalgarnokenneth biosyntheticpclgraftcollagenbulkmaterialfortissueengineeringapplications AT ferreiraanamarina biosyntheticpclgraftcollagenbulkmaterialfortissueengineeringapplications |