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Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications

Biosynthetic materials have emerged as one of the most exciting and productive fields in polymer chemistry due to their widespread adoption and potential applications in tissue engineering (TE) research. In this work, we report the synthesis of a poly(ε-caprolactone)-graft-collagen (PCL-g-Coll) copo...

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Autores principales: Gentile, Piergiorgio, McColgan-Bannon, Kegan, Gianone, Nicolò Ceretto, Sefat, Farshid, Dalgarno, Kenneth, Ferreira, Ana Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551736/
https://www.ncbi.nlm.nih.gov/pubmed/28773053
http://dx.doi.org/10.3390/ma10070693
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author Gentile, Piergiorgio
McColgan-Bannon, Kegan
Gianone, Nicolò Ceretto
Sefat, Farshid
Dalgarno, Kenneth
Ferreira, Ana Marina
author_facet Gentile, Piergiorgio
McColgan-Bannon, Kegan
Gianone, Nicolò Ceretto
Sefat, Farshid
Dalgarno, Kenneth
Ferreira, Ana Marina
author_sort Gentile, Piergiorgio
collection PubMed
description Biosynthetic materials have emerged as one of the most exciting and productive fields in polymer chemistry due to their widespread adoption and potential applications in tissue engineering (TE) research. In this work, we report the synthesis of a poly(ε-caprolactone)-graft-collagen (PCL-g-Coll) copolymer. We combine its good mechanical and biodegradable PCL properties with the great biological properties of type I collagen as a functional material for TE. PCL, previously dissolved in dimethylformamide/dichloromethane mixture, and reacted with collagen using carbodiimide coupling chemistry. The synthesised material was characterised physically, chemically and biologically, using pure PCL and PCL/Coll blend samples as control. Infrared spectroscopy evidenced the presence of amide I and II peaks for the conjugated material. Similarly, XPS evidenced the presence of C–N and N–C=O bonds (8.96 ± 2.02% and 8.52 ± 0.63%; respectively) for PCL-g-Coll. Static contact angles showed a slight decrease in the conjugated sample. However, good biocompatibility and metabolic activity was obtained on PCL-g-Coll films compared to PCL and blend controls. After 3 days of culture, fibroblasts exhibited a spindle-like morphology, spreading homogeneously along the PCL-g-Coll film surface. We have engineered a functional biosynthetic polymer that can be processed by electrospinning.
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spelling pubmed-55517362017-08-11 Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications Gentile, Piergiorgio McColgan-Bannon, Kegan Gianone, Nicolò Ceretto Sefat, Farshid Dalgarno, Kenneth Ferreira, Ana Marina Materials (Basel) Article Biosynthetic materials have emerged as one of the most exciting and productive fields in polymer chemistry due to their widespread adoption and potential applications in tissue engineering (TE) research. In this work, we report the synthesis of a poly(ε-caprolactone)-graft-collagen (PCL-g-Coll) copolymer. We combine its good mechanical and biodegradable PCL properties with the great biological properties of type I collagen as a functional material for TE. PCL, previously dissolved in dimethylformamide/dichloromethane mixture, and reacted with collagen using carbodiimide coupling chemistry. The synthesised material was characterised physically, chemically and biologically, using pure PCL and PCL/Coll blend samples as control. Infrared spectroscopy evidenced the presence of amide I and II peaks for the conjugated material. Similarly, XPS evidenced the presence of C–N and N–C=O bonds (8.96 ± 2.02% and 8.52 ± 0.63%; respectively) for PCL-g-Coll. Static contact angles showed a slight decrease in the conjugated sample. However, good biocompatibility and metabolic activity was obtained on PCL-g-Coll films compared to PCL and blend controls. After 3 days of culture, fibroblasts exhibited a spindle-like morphology, spreading homogeneously along the PCL-g-Coll film surface. We have engineered a functional biosynthetic polymer that can be processed by electrospinning. MDPI 2017-06-23 /pmc/articles/PMC5551736/ /pubmed/28773053 http://dx.doi.org/10.3390/ma10070693 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gentile, Piergiorgio
McColgan-Bannon, Kegan
Gianone, Nicolò Ceretto
Sefat, Farshid
Dalgarno, Kenneth
Ferreira, Ana Marina
Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications
title Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications
title_full Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications
title_fullStr Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications
title_full_unstemmed Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications
title_short Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications
title_sort biosynthetic pcl-graft-collagen bulk material for tissue engineering applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551736/
https://www.ncbi.nlm.nih.gov/pubmed/28773053
http://dx.doi.org/10.3390/ma10070693
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