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The Impact of Metal Ion Exposure on the Cellular Behavior of Human Osteoblasts and PBMCs: In Vitro Analyses of Osteolytic Processes

Osteolysis in the periprosthetic tissue can be caused by metallic wear particles and ions that can originate from implant surface corrosion. These products influence cellular behavior and stimulate the expression of proinflammatory cytokines. The purpose of this study was to evaluate the impact of C...

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Autores principales: Jonitz-Heincke, Anika, Tillmann, Jenny, Klinder, Annett, Krueger, Simone, Kretzer, Jan Philippe, Høl, Paul Johan, Paulus, Alexander C., Bader, Rainer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551777/
https://www.ncbi.nlm.nih.gov/pubmed/28773099
http://dx.doi.org/10.3390/ma10070734
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author Jonitz-Heincke, Anika
Tillmann, Jenny
Klinder, Annett
Krueger, Simone
Kretzer, Jan Philippe
Høl, Paul Johan
Paulus, Alexander C.
Bader, Rainer
author_facet Jonitz-Heincke, Anika
Tillmann, Jenny
Klinder, Annett
Krueger, Simone
Kretzer, Jan Philippe
Høl, Paul Johan
Paulus, Alexander C.
Bader, Rainer
author_sort Jonitz-Heincke, Anika
collection PubMed
description Osteolysis in the periprosthetic tissue can be caused by metallic wear particles and ions that can originate from implant surface corrosion. These products influence cellular behavior and stimulate the expression of proinflammatory cytokines. The purpose of this study was to evaluate the impact of CoCr29Mo6 ions on cell survival, differentiation, and cytokine expression in human osteoblasts and peripheral blood mononuclear cells (PBMCs). Thus, we exposed cells with a mixture of 200 µg/L ion solution and determined cell viability and apoptosis/necrosis. Gene expression analyses of osteoblastic and osteoclastic differentiation markers as well as pro-osteolytic mediators (IL-6, IL-8, TNF-α, MCP-1, MMP1, TIMP1) were performed. These markers were also investigated in mixed cultures of adherent and non-adherent PBMCs as well as in co-cultures of human osteoblasts and PBMCs. The ion solution induced necrosis in osteoblasts and PBMCs in single cultures. All examined mediators were highly expressed in the co-culture of osteoblasts and PBMCs whereas in the single cell cultures only IL-6, IL-8, and MMP1 were found to be stimulated. While the applied concentration of the CoCr29Mo6 ion solutions had only marginal effects on human osteoblasts and PBMCs alone, the co-culture may provide a comprehensive model to study osteolytic processes in response to Co and Cr ions.
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spelling pubmed-55517772017-08-11 The Impact of Metal Ion Exposure on the Cellular Behavior of Human Osteoblasts and PBMCs: In Vitro Analyses of Osteolytic Processes Jonitz-Heincke, Anika Tillmann, Jenny Klinder, Annett Krueger, Simone Kretzer, Jan Philippe Høl, Paul Johan Paulus, Alexander C. Bader, Rainer Materials (Basel) Article Osteolysis in the periprosthetic tissue can be caused by metallic wear particles and ions that can originate from implant surface corrosion. These products influence cellular behavior and stimulate the expression of proinflammatory cytokines. The purpose of this study was to evaluate the impact of CoCr29Mo6 ions on cell survival, differentiation, and cytokine expression in human osteoblasts and peripheral blood mononuclear cells (PBMCs). Thus, we exposed cells with a mixture of 200 µg/L ion solution and determined cell viability and apoptosis/necrosis. Gene expression analyses of osteoblastic and osteoclastic differentiation markers as well as pro-osteolytic mediators (IL-6, IL-8, TNF-α, MCP-1, MMP1, TIMP1) were performed. These markers were also investigated in mixed cultures of adherent and non-adherent PBMCs as well as in co-cultures of human osteoblasts and PBMCs. The ion solution induced necrosis in osteoblasts and PBMCs in single cultures. All examined mediators were highly expressed in the co-culture of osteoblasts and PBMCs whereas in the single cell cultures only IL-6, IL-8, and MMP1 were found to be stimulated. While the applied concentration of the CoCr29Mo6 ion solutions had only marginal effects on human osteoblasts and PBMCs alone, the co-culture may provide a comprehensive model to study osteolytic processes in response to Co and Cr ions. MDPI 2017-07-03 /pmc/articles/PMC5551777/ /pubmed/28773099 http://dx.doi.org/10.3390/ma10070734 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jonitz-Heincke, Anika
Tillmann, Jenny
Klinder, Annett
Krueger, Simone
Kretzer, Jan Philippe
Høl, Paul Johan
Paulus, Alexander C.
Bader, Rainer
The Impact of Metal Ion Exposure on the Cellular Behavior of Human Osteoblasts and PBMCs: In Vitro Analyses of Osteolytic Processes
title The Impact of Metal Ion Exposure on the Cellular Behavior of Human Osteoblasts and PBMCs: In Vitro Analyses of Osteolytic Processes
title_full The Impact of Metal Ion Exposure on the Cellular Behavior of Human Osteoblasts and PBMCs: In Vitro Analyses of Osteolytic Processes
title_fullStr The Impact of Metal Ion Exposure on the Cellular Behavior of Human Osteoblasts and PBMCs: In Vitro Analyses of Osteolytic Processes
title_full_unstemmed The Impact of Metal Ion Exposure on the Cellular Behavior of Human Osteoblasts and PBMCs: In Vitro Analyses of Osteolytic Processes
title_short The Impact of Metal Ion Exposure on the Cellular Behavior of Human Osteoblasts and PBMCs: In Vitro Analyses of Osteolytic Processes
title_sort impact of metal ion exposure on the cellular behavior of human osteoblasts and pbmcs: in vitro analyses of osteolytic processes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551777/
https://www.ncbi.nlm.nih.gov/pubmed/28773099
http://dx.doi.org/10.3390/ma10070734
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