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Hydrothermal Sterilization Improves Initial Osteoblast Responses on Sandpaper-Polished Titanium

Hydrocarbon contamination accumulated on titanium (Ti) implant surfaces during storage and sterilization is unavoidable and difficult to remove. It impairs the bioactivity of implants, restricts initial interactions between implants and the surrounding biological environment, and has become a common...

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Detalles Bibliográficos
Autores principales: Shi, Xingling, Xu, Lingli, Wang, Qingliang, Sunarso, Xu, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551855/
https://www.ncbi.nlm.nih.gov/pubmed/28773174
http://dx.doi.org/10.3390/ma10070812
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author Shi, Xingling
Xu, Lingli
Wang, Qingliang
Sunarso,
Xu, Lin
author_facet Shi, Xingling
Xu, Lingli
Wang, Qingliang
Sunarso,
Xu, Lin
author_sort Shi, Xingling
collection PubMed
description Hydrocarbon contamination accumulated on titanium (Ti) implant surfaces during storage and sterilization is unavoidable and difficult to remove. It impairs the bioactivity of implants, restricts initial interactions between implants and the surrounding biological environment, and has become a common challenge for Ti implants. To overcome this problem, sterilization was considered as the final surface modification and a novel method, hydrothermal sterilization (HS), was proposed. Briefly, stored sandpaper-polished Ti specimens were sterilized in a glass container with pure water at 121 °C for 20 min and kept in the same water until utilization. As a control, another group of specimens was sterilized with conventional autoclaving (AC) at 121 °C for 20 min and stored in sterilization pouches after being dried at 60 °C. Compared with AC, HS deposited numerous nano-sized particles on the substrates, reduced the atomic percentage of the surface carbon, and transformed the Ti surface to a super hydrophilic status. HS also increased the attachment rate, spread, proliferation, and the mineralized nodule areas of rat bone marrow-derived osteoblasts. These results suggest that HS enhances the bioactivity of Ti implants for osteoblasts, and that this biofunctionalization was attributed to nanostructure construction, hydrophilic conversion, and the effective removal of hydrocarbons. Hydrothermal sterilization is proposed to be used as a universal sterilization method for all kinds of titanium implants without apatite coating.
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spelling pubmed-55518552017-08-11 Hydrothermal Sterilization Improves Initial Osteoblast Responses on Sandpaper-Polished Titanium Shi, Xingling Xu, Lingli Wang, Qingliang Sunarso, Xu, Lin Materials (Basel) Article Hydrocarbon contamination accumulated on titanium (Ti) implant surfaces during storage and sterilization is unavoidable and difficult to remove. It impairs the bioactivity of implants, restricts initial interactions between implants and the surrounding biological environment, and has become a common challenge for Ti implants. To overcome this problem, sterilization was considered as the final surface modification and a novel method, hydrothermal sterilization (HS), was proposed. Briefly, stored sandpaper-polished Ti specimens were sterilized in a glass container with pure water at 121 °C for 20 min and kept in the same water until utilization. As a control, another group of specimens was sterilized with conventional autoclaving (AC) at 121 °C for 20 min and stored in sterilization pouches after being dried at 60 °C. Compared with AC, HS deposited numerous nano-sized particles on the substrates, reduced the atomic percentage of the surface carbon, and transformed the Ti surface to a super hydrophilic status. HS also increased the attachment rate, spread, proliferation, and the mineralized nodule areas of rat bone marrow-derived osteoblasts. These results suggest that HS enhances the bioactivity of Ti implants for osteoblasts, and that this biofunctionalization was attributed to nanostructure construction, hydrophilic conversion, and the effective removal of hydrocarbons. Hydrothermal sterilization is proposed to be used as a universal sterilization method for all kinds of titanium implants without apatite coating. MDPI 2017-07-17 /pmc/articles/PMC5551855/ /pubmed/28773174 http://dx.doi.org/10.3390/ma10070812 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shi, Xingling
Xu, Lingli
Wang, Qingliang
Sunarso,
Xu, Lin
Hydrothermal Sterilization Improves Initial Osteoblast Responses on Sandpaper-Polished Titanium
title Hydrothermal Sterilization Improves Initial Osteoblast Responses on Sandpaper-Polished Titanium
title_full Hydrothermal Sterilization Improves Initial Osteoblast Responses on Sandpaper-Polished Titanium
title_fullStr Hydrothermal Sterilization Improves Initial Osteoblast Responses on Sandpaper-Polished Titanium
title_full_unstemmed Hydrothermal Sterilization Improves Initial Osteoblast Responses on Sandpaper-Polished Titanium
title_short Hydrothermal Sterilization Improves Initial Osteoblast Responses on Sandpaper-Polished Titanium
title_sort hydrothermal sterilization improves initial osteoblast responses on sandpaper-polished titanium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551855/
https://www.ncbi.nlm.nih.gov/pubmed/28773174
http://dx.doi.org/10.3390/ma10070812
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AT sunarso hydrothermalsterilizationimprovesinitialosteoblastresponsesonsandpaperpolishedtitanium
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