Cargando…

Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts

Human amniotic membrane extracts contain numerous growth factors and bioactive substances. However, osteogenic effects of amnion and chorion membrane extracts (AME and CME, respectively) on osteoblasts are unclear. In this study, we explored the ability of AME and CME to promote the osteogenic diffe...

Descripción completa

Detalles Bibliográficos
Autores principales: Go, Yoon Young, Kim, Sung Eun, Cho, Geum Joon, Chae, Sung-Won, Song, Jae-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552222/
https://www.ncbi.nlm.nih.gov/pubmed/28797129
http://dx.doi.org/10.1371/journal.pone.0182716
_version_ 1783256434202378240
author Go, Yoon Young
Kim, Sung Eun
Cho, Geum Joon
Chae, Sung-Won
Song, Jae-Jun
author_facet Go, Yoon Young
Kim, Sung Eun
Cho, Geum Joon
Chae, Sung-Won
Song, Jae-Jun
author_sort Go, Yoon Young
collection PubMed
description Human amniotic membrane extracts contain numerous growth factors and bioactive substances. However, osteogenic effects of amnion and chorion membrane extracts (AME and CME, respectively) on osteoblasts are unclear. In this study, we explored the ability of AME and CME to promote the osteogenic differentiation of osteoblast-like MG-63 cells. MG-63 cells were cultured in osteogenic induction medium (OIM) with or without exogenous AME and CME. CME enhanced the osteogenic differentiation of MG-63 cells compared with AME, as indicated by increased mineralization; alkaline phosphatase activity; and mRNA expression of osteogenic marker genes encoding integrin-binding sialoprotein (IBSP), RUNX2, OSTERIX, and osteocalcin (OCN). Interestingly, AME and CME contained different combinations of osteogenesis-related growth factors, including basic fibroblast growth factor (bFGF), transforming growth factor beta-1 (TGFβ-1), and epidermal growth factor (EGF), which differentially regulated the osteogenic differentiation of MG-63 cells. bFGF and TGFβ-1 present in CME positively regulated the osteogenic differentiation of MG-63 cells, whereas EGF present in AME negatively regulated the differentiation of MG-63 cells. Moreover, exogenous treatment of EGF antagonized CME-induced mineralization of extracellular matrix on MG-63 cells. We compared the osteogenic efficacy of CME with that of BMP2, bFGF, and TGFβ-1 alone or their combinations. We observed that CME greatly enhanced osteogenesis by providing a conductive environment for the differentiation of MG-63 cells. Together, our results indicated that human AME and CME exerted differential effects on osteogenesis because of the presence of different compositions of growth factors. In addition, our results highlighted a new possible strategy of using CME as a biocompatible therapeutic material for bone regeneration.
format Online
Article
Text
id pubmed-5552222
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-55522222017-08-25 Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts Go, Yoon Young Kim, Sung Eun Cho, Geum Joon Chae, Sung-Won Song, Jae-Jun PLoS One Research Article Human amniotic membrane extracts contain numerous growth factors and bioactive substances. However, osteogenic effects of amnion and chorion membrane extracts (AME and CME, respectively) on osteoblasts are unclear. In this study, we explored the ability of AME and CME to promote the osteogenic differentiation of osteoblast-like MG-63 cells. MG-63 cells were cultured in osteogenic induction medium (OIM) with or without exogenous AME and CME. CME enhanced the osteogenic differentiation of MG-63 cells compared with AME, as indicated by increased mineralization; alkaline phosphatase activity; and mRNA expression of osteogenic marker genes encoding integrin-binding sialoprotein (IBSP), RUNX2, OSTERIX, and osteocalcin (OCN). Interestingly, AME and CME contained different combinations of osteogenesis-related growth factors, including basic fibroblast growth factor (bFGF), transforming growth factor beta-1 (TGFβ-1), and epidermal growth factor (EGF), which differentially regulated the osteogenic differentiation of MG-63 cells. bFGF and TGFβ-1 present in CME positively regulated the osteogenic differentiation of MG-63 cells, whereas EGF present in AME negatively regulated the differentiation of MG-63 cells. Moreover, exogenous treatment of EGF antagonized CME-induced mineralization of extracellular matrix on MG-63 cells. We compared the osteogenic efficacy of CME with that of BMP2, bFGF, and TGFβ-1 alone or their combinations. We observed that CME greatly enhanced osteogenesis by providing a conductive environment for the differentiation of MG-63 cells. Together, our results indicated that human AME and CME exerted differential effects on osteogenesis because of the presence of different compositions of growth factors. In addition, our results highlighted a new possible strategy of using CME as a biocompatible therapeutic material for bone regeneration. Public Library of Science 2017-08-10 /pmc/articles/PMC5552222/ /pubmed/28797129 http://dx.doi.org/10.1371/journal.pone.0182716 Text en © 2017 Go et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Go, Yoon Young
Kim, Sung Eun
Cho, Geum Joon
Chae, Sung-Won
Song, Jae-Jun
Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
title Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
title_full Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
title_fullStr Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
title_full_unstemmed Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
title_short Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
title_sort differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552222/
https://www.ncbi.nlm.nih.gov/pubmed/28797129
http://dx.doi.org/10.1371/journal.pone.0182716
work_keys_str_mv AT goyoonyoung differentialeffectsofamnionandchorionmembraneextractsonosteoblastlikecellsduetothedifferentgrowthfactorcompositionoftheextracts
AT kimsungeun differentialeffectsofamnionandchorionmembraneextractsonosteoblastlikecellsduetothedifferentgrowthfactorcompositionoftheextracts
AT chogeumjoon differentialeffectsofamnionandchorionmembraneextractsonosteoblastlikecellsduetothedifferentgrowthfactorcompositionoftheextracts
AT chaesungwon differentialeffectsofamnionandchorionmembraneextractsonosteoblastlikecellsduetothedifferentgrowthfactorcompositionoftheextracts
AT songjaejun differentialeffectsofamnionandchorionmembraneextractsonosteoblastlikecellsduetothedifferentgrowthfactorcompositionoftheextracts