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Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system
Magnesium (Mg)-based stents are extensively explored to alleviate atherosclerosis due to their biodegradability and relative hemocompatibility. To ensure the quality, safety and cost-efficacy of bioresorbable scaffolds and full utilization of the material tunability afforded by alloying, it is criti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552284/ https://www.ncbi.nlm.nih.gov/pubmed/28797069 http://dx.doi.org/10.1371/journal.pone.0182914 |
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author | Liu, Lumei Koo, Youngmi Collins, Boyce Xu, Zhigang Sankar, Jagannathan Yun, Yeoheung |
author_facet | Liu, Lumei Koo, Youngmi Collins, Boyce Xu, Zhigang Sankar, Jagannathan Yun, Yeoheung |
author_sort | Liu, Lumei |
collection | PubMed |
description | Magnesium (Mg)-based stents are extensively explored to alleviate atherosclerosis due to their biodegradability and relative hemocompatibility. To ensure the quality, safety and cost-efficacy of bioresorbable scaffolds and full utilization of the material tunability afforded by alloying, it is critical to access degradability and thrombosis potential of Mg-based alloys using improved in vitro models that mimic as closely as possible the in vivo microenvironment. In this study, we investigated biodegradation and initial thrombogenic behavior of Mg-based alloys at the interface between Mg alloys’ surface and simulated physiological environment using a microfluidic system. The degradation properties of Mg-based alloys WE43, AZ31, ZWEK-L, and ZWEK-C were evaluated in complete culture medium and their thrombosis potentials in platelet rich plasma, respectively. The results show that 1) physiological shear stress increased the corrosion rate and decreased platelets adhesion rate as compared to static immersion; 2) secondary phases and impurities in material composition induced galvanic corrosion, resulting in higher corrosion resistance and platelet adhesion rate; 3) Mg-based alloys with higher corrosion rate showed higher platelets adhesion rate. We conclude that a microfluidic-based in vitro system allows evaluation of biodegradation behaviors and platelets responses of Mg-based alloys under specific shear stress, and degradability is related to platelets adhesion. |
format | Online Article Text |
id | pubmed-5552284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-55522842017-08-25 Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system Liu, Lumei Koo, Youngmi Collins, Boyce Xu, Zhigang Sankar, Jagannathan Yun, Yeoheung PLoS One Research Article Magnesium (Mg)-based stents are extensively explored to alleviate atherosclerosis due to their biodegradability and relative hemocompatibility. To ensure the quality, safety and cost-efficacy of bioresorbable scaffolds and full utilization of the material tunability afforded by alloying, it is critical to access degradability and thrombosis potential of Mg-based alloys using improved in vitro models that mimic as closely as possible the in vivo microenvironment. In this study, we investigated biodegradation and initial thrombogenic behavior of Mg-based alloys at the interface between Mg alloys’ surface and simulated physiological environment using a microfluidic system. The degradation properties of Mg-based alloys WE43, AZ31, ZWEK-L, and ZWEK-C were evaluated in complete culture medium and their thrombosis potentials in platelet rich plasma, respectively. The results show that 1) physiological shear stress increased the corrosion rate and decreased platelets adhesion rate as compared to static immersion; 2) secondary phases and impurities in material composition induced galvanic corrosion, resulting in higher corrosion resistance and platelet adhesion rate; 3) Mg-based alloys with higher corrosion rate showed higher platelets adhesion rate. We conclude that a microfluidic-based in vitro system allows evaluation of biodegradation behaviors and platelets responses of Mg-based alloys under specific shear stress, and degradability is related to platelets adhesion. Public Library of Science 2017-08-10 /pmc/articles/PMC5552284/ /pubmed/28797069 http://dx.doi.org/10.1371/journal.pone.0182914 Text en © 2017 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Liu, Lumei Koo, Youngmi Collins, Boyce Xu, Zhigang Sankar, Jagannathan Yun, Yeoheung Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system |
title | Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system |
title_full | Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system |
title_fullStr | Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system |
title_full_unstemmed | Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system |
title_short | Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system |
title_sort | biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552284/ https://www.ncbi.nlm.nih.gov/pubmed/28797069 http://dx.doi.org/10.1371/journal.pone.0182914 |
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