Compartmentalized embryoid body culture for induction of spatially patterned differentiation

We developed a compartmentalized culture system of single embryoid bodies (EBs) utilizing a through-hole on a membrane to induce spatially patterned differentiation. An EB derived from mouse pluripotent stem cells was immobilized on the through-hole. By introducing a stem cell maintenance medium and...

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Detalles Bibliográficos
Autores principales: Kaneda, Shohei, Kawada, Jiro, Akutsu, Hidenori, Ichida, Justin, Ikeuchi, Yoshiho, Fujii, Teruo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2017
Materias:
Fast Track
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552387/
https://www.ncbi.nlm.nih.gov/pubmed/28852428
http://dx.doi.org/10.1063/1.4994989
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author Kaneda, Shohei
Kawada, Jiro
Akutsu, Hidenori
Ichida, Justin
Ikeuchi, Yoshiho
Fujii, Teruo
author_facet Kaneda, Shohei
Kawada, Jiro
Akutsu, Hidenori
Ichida, Justin
Ikeuchi, Yoshiho
Fujii, Teruo
author_sort Kaneda, Shohei
collection PubMed
description We developed a compartmentalized culture system of single embryoid bodies (EBs) utilizing a through-hole on a membrane to induce spatially patterned differentiation. An EB derived from mouse pluripotent stem cells was immobilized on the through-hole. By introducing a stem cell maintenance medium and a differentiation medium into upper and lower culture compartments, respectively, a localized differentiated state was achieved only in the lower part of EB, which is exposed to the medium in the lower compartment. This system may enable us to reconstruct complex tissues and to recapitulate developmental processes using EBs.
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spelling pubmed-55523872017-08-29 Compartmentalized embryoid body culture for induction of spatially patterned differentiation Kaneda, Shohei Kawada, Jiro Akutsu, Hidenori Ichida, Justin Ikeuchi, Yoshiho Fujii, Teruo Biomicrofluidics Fast Track We developed a compartmentalized culture system of single embryoid bodies (EBs) utilizing a through-hole on a membrane to induce spatially patterned differentiation. An EB derived from mouse pluripotent stem cells was immobilized on the through-hole. By introducing a stem cell maintenance medium and a differentiation medium into upper and lower culture compartments, respectively, a localized differentiated state was achieved only in the lower part of EB, which is exposed to the medium in the lower compartment. This system may enable us to reconstruct complex tissues and to recapitulate developmental processes using EBs. AIP Publishing LLC 2017-08-02 /pmc/articles/PMC5552387/ /pubmed/28852428 http://dx.doi.org/10.1063/1.4994989 Text en © 2017 Author(s). 1932-1058/2017/11(4)/041101/4 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Fast Track
Kaneda, Shohei
Kawada, Jiro
Akutsu, Hidenori
Ichida, Justin
Ikeuchi, Yoshiho
Fujii, Teruo
Compartmentalized embryoid body culture for induction of spatially patterned differentiation
title Compartmentalized embryoid body culture for induction of spatially patterned differentiation
title_full Compartmentalized embryoid body culture for induction of spatially patterned differentiation
title_fullStr Compartmentalized embryoid body culture for induction of spatially patterned differentiation
title_full_unstemmed Compartmentalized embryoid body culture for induction of spatially patterned differentiation
title_short Compartmentalized embryoid body culture for induction of spatially patterned differentiation
title_sort compartmentalized embryoid body culture for induction of spatially patterned differentiation
topic Fast Track
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552387/
https://www.ncbi.nlm.nih.gov/pubmed/28852428
http://dx.doi.org/10.1063/1.4994989
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