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A novel functional gene associated with cold tolerance at the seedling stage in rice

Identification and cloning of cold‐tolerant genes that can stably express under different cold environments are crucial for molecular rice breeding for cold tolerance. In the previous study, we identified a cold‐tolerant QTL at the seedling stage, qCTS‐9 which could be detected under different cold...

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Autores principales: Zhao, Junliang, Zhang, Shaohong, Dong, Jingfang, Yang, Tifeng, Mao, Xingxue, Liu, Qing, Wang, Xiaofei, Liu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552475/
https://www.ncbi.nlm.nih.gov/pubmed/28173633
http://dx.doi.org/10.1111/pbi.12704
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author Zhao, Junliang
Zhang, Shaohong
Dong, Jingfang
Yang, Tifeng
Mao, Xingxue
Liu, Qing
Wang, Xiaofei
Liu, Bin
author_facet Zhao, Junliang
Zhang, Shaohong
Dong, Jingfang
Yang, Tifeng
Mao, Xingxue
Liu, Qing
Wang, Xiaofei
Liu, Bin
author_sort Zhao, Junliang
collection PubMed
description Identification and cloning of cold‐tolerant genes that can stably express under different cold environments are crucial for molecular rice breeding for cold tolerance. In the previous study, we identified a cold‐tolerant QTL at the seedling stage, qCTS‐9 which could be detected under different cold environments using a recombinant inbred line (RIL) population derived from a cold‐tolerant variety Lijiangxintuanheigu (LTH) and a cold‐sensitive variety Shanhuangzhan 2 (SHZ‐2). In this study, eight candidate genes within the qCTS‐9 interval were identified through integrated analysis of QTL mapping with genomewide differential expression profiling of LTH. The qRT‐PCR assay showed that only Os09g0410300 exhibited different expression patterns between LTH and SHZ‐2 during cold stress, and significantly positive correlation was found between cold induction of Os09g0410300 and seedling cold tolerance in the RI lines. Five SNPs and one InDel in the promoters of Os09g0410300 were detected between LTH and SHZ‐2, and the InDel marker ID410300 designed based on the insertion–deletion polymorphism in the promoter was significantly associated with seedling cold tolerance in RIL population. Further, Os09g0410300 over‐expression plants exhibited enhanced cold tolerance at the seedling stage compared with the wild‐type plants. Thus, our results suggest that Os09g0410300 is the functional gene underlying qCTS‐9. To our knowledge, it is a novel gene contributed to enhance cold tolerance at the seedling stage in rice. Identification of the functional gene underlying qCTS‐9 and development of the gene‐specific marker will facilitate molecular breeding for cold tolerance at the seedling stage in rice through transgenic approach and marker‐assisted selection (MAS).
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spelling pubmed-55524752017-08-25 A novel functional gene associated with cold tolerance at the seedling stage in rice Zhao, Junliang Zhang, Shaohong Dong, Jingfang Yang, Tifeng Mao, Xingxue Liu, Qing Wang, Xiaofei Liu, Bin Plant Biotechnol J Research Articles Identification and cloning of cold‐tolerant genes that can stably express under different cold environments are crucial for molecular rice breeding for cold tolerance. In the previous study, we identified a cold‐tolerant QTL at the seedling stage, qCTS‐9 which could be detected under different cold environments using a recombinant inbred line (RIL) population derived from a cold‐tolerant variety Lijiangxintuanheigu (LTH) and a cold‐sensitive variety Shanhuangzhan 2 (SHZ‐2). In this study, eight candidate genes within the qCTS‐9 interval were identified through integrated analysis of QTL mapping with genomewide differential expression profiling of LTH. The qRT‐PCR assay showed that only Os09g0410300 exhibited different expression patterns between LTH and SHZ‐2 during cold stress, and significantly positive correlation was found between cold induction of Os09g0410300 and seedling cold tolerance in the RI lines. Five SNPs and one InDel in the promoters of Os09g0410300 were detected between LTH and SHZ‐2, and the InDel marker ID410300 designed based on the insertion–deletion polymorphism in the promoter was significantly associated with seedling cold tolerance in RIL population. Further, Os09g0410300 over‐expression plants exhibited enhanced cold tolerance at the seedling stage compared with the wild‐type plants. Thus, our results suggest that Os09g0410300 is the functional gene underlying qCTS‐9. To our knowledge, it is a novel gene contributed to enhance cold tolerance at the seedling stage in rice. Identification of the functional gene underlying qCTS‐9 and development of the gene‐specific marker will facilitate molecular breeding for cold tolerance at the seedling stage in rice through transgenic approach and marker‐assisted selection (MAS). John Wiley and Sons Inc. 2017-03-30 2017-09 /pmc/articles/PMC5552475/ /pubmed/28173633 http://dx.doi.org/10.1111/pbi.12704 Text en © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Zhao, Junliang
Zhang, Shaohong
Dong, Jingfang
Yang, Tifeng
Mao, Xingxue
Liu, Qing
Wang, Xiaofei
Liu, Bin
A novel functional gene associated with cold tolerance at the seedling stage in rice
title A novel functional gene associated with cold tolerance at the seedling stage in rice
title_full A novel functional gene associated with cold tolerance at the seedling stage in rice
title_fullStr A novel functional gene associated with cold tolerance at the seedling stage in rice
title_full_unstemmed A novel functional gene associated with cold tolerance at the seedling stage in rice
title_short A novel functional gene associated with cold tolerance at the seedling stage in rice
title_sort novel functional gene associated with cold tolerance at the seedling stage in rice
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552475/
https://www.ncbi.nlm.nih.gov/pubmed/28173633
http://dx.doi.org/10.1111/pbi.12704
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